Inhibitory Effects of Some Plant Essential Oils Against Arcobacter butzleri and Potential for Rosemary Oil as a Natural Food Preservative

Reyhan Irkin,Secil Abay,Fuat Aydin 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.3

We investigated the inhibitory activity of commercially marketed essential oils of mint, rosemary, orange, sage, cinnamon, bay, clove, and cumin against Arcobacter butzleri and Arcobacter skirrowii and the effects of the essential oil of rosemary against A. butzleri in a cooked minced beef system. Using the disc diffusion method to determine the inhibitory activities of these plant essential oils against strains of Arcobacter, we found that those of rosemary, bay, cinnamon, and clove had strong inhibitory activity against these organisms, whereas the essential oils of cumin, mint, and sage failed to show inhibitory activity against most of the Arcobacter strains tested. The 0.5% (vol/wt) essential oil of rosemary was completely inhibitory against A. butzleri in the cooked minced beef system at 4°C. These essential oils may be further investigated as a natural solution to the food industry by creating an additional barrier (hurdle technology) to inhibit the growth of Arcobacter strains.

Enhancing the Thermostability of Cellulase from Clostridium thermocellum via Salt Bridge Interactions

Reyhane Zamani,Sayyed Shahryar Rahpeyma,Moein Aliakbari,Mousa Naderi,Mohsen Yazdanei,Saeed Aminzadeh,Jafar Khezri,Kamahldin Haghbeen,Ali Asghar Karkhane 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.4

Improving the thermal stability of enzymes is an essential factor in the industrial applications of enzymes. Many methods related to increased thermal stability were explained, and increasing salt bridges is one of the strategies for improving the thermal stability of enzymes. In this study, mutations T59E, I145R, N149R, V219D, and A262R are introduced into the native cellulase gene to produce the mutant 5M-cel5E cellulase. In silico results showed that the mutation increased the salt bridges from 15 to 28. Root mean square fluctuation (RMSF) calculations confirmed that the mutation increased protein stability. Furthermore, the docking results showed that the affinity of cellobiose for the 5M-cel5E active site (-122.759) was slightly decreased compared to native cellulase (-130.93). No enzymatic activity was found in 5M-cel5E cellulase after cloning, expression and purification. Activated the enzyme with a back mutation of R149N, the result of which was named 4M-cel5E. The last mutation increases the salt bridges from 15 to 22, creating 4 salt bridge networks. The 4M-cel5E enzyme exhibited a maximum activity of 463 U/mg at pH 6.0 and 45°C. The mutations also increased the enzyme thermal stability up to 1.5 and 3.4-fold at temperatures of 65 and 67oC, respectively. These mutations made the Clostridium thermocellum cellulase suitable for various industries such the biofuel and paper.

Senneh Gelim: The Magnificent Living Carpet Tradition of Iranian Kurdish Women

Reyhane Mirabootalebi 계명대학교 실크로드 중앙아시아연구원 2023 Acta Via Serica Vol.8 No.1

Traditional Kurdish weavings are among the world’s most ancient living textile traditions. One of the largest regional ethnic and linguistic groups, Kurds have inhabited a significant part of Western Asia for millennia. Historically, Kurdish territories were crisscrossed by old and important trade routes, including the Silk Roads. This led to the formation of some of the most significant Kurdish artistic and cultural traditions, including textiles, which influenced and were influenced by those of other non-Kurdish ethnic groups from Caucasia to Central Asia and beyond. One example of Kurdish carpet traditions born in the eighteenth century at the cross-sections of Safavid (1501-1736) urban carpets workshops and centuries-old indigenous Kurdish tribal/rural weaves is senneh gelim or sojaee. A finely flatwoven carpet that was exchanged regionally and internationally as a diplomatic gift and a highly prized commodity. Although in decline, senneh gelims continue to be made by Kurdish women weavers in their original birthplace Sanandaj, the provincial capital of Iranian Kurdistan to date. This study adopts an inter-disciplinary approach to present an image of senneh gelim and women gelim weavers, tracing the developmental trajectories of the craft from the eighteenth century to the present time by drawing on extant art-historical and social scientific studies along with primary ethnographic data collected in Iranian Kurdistan (2018-2019). It investigates the craft tradition’s historical origin, various aspects such as techniques, materials, aesthetics, functions, and meanings, and how these transformed over time. Additionally, the paper looks at the social contexts of production, focusing on women carpet weavers and how their socioeconomic and cultural situation has formed senneh carpet production in the past and present and the implications for long-term preservation.

High tendency to the substantial concern on body shape and eating disorders risk of the students majoring Nutrition or Sport Sciences

Reyhan Nergiz-Unal,Pelin Bilgic,Nurcan Yabancı 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.6

BACKGROUND/OBJECTIVES: Studies have indicated that university students majoring in nutrition and dietetics or sport sciences may have more obsessions associated with eating attitudes and body shape perception compared to other disciplines i.e. social sciences. Therefore, this study aimed to assess and compare the risk of eating disorders and body shape perception. MATERIALS/METHODS: Data was collected from 773 undergraduate students at the Departments of Nutrition and Dietetics (NDD) (n = 254), Physical Education and Sports (PESD) (n = 263), and Social Sciences (SOC) (n = 256).A socio-demographic and personal information questionnaire, Eating Attitudes Test (EAT-40), Body Shape Questionnaire (BSQ-34), Perceived Figure Rating Scale (FRS) were applied; and body weights and heights were measured. RESULTS: Mean EAT-40 scores showed that, both male and female students of PESD had the highest scores (17.4 ± 11.6) compared with NDD (14.3 ± 8.3) and SOC (13.0 ± 6.2) (P < 0.05). According to EAT-40 classification, high risk in abnormal eating behavior was more in PESD (10.7%) compared to NDD (2.9%) and SOC (0.4%) students (P < 0.05). Students of PESD, who skipped meal, had higher tendency to the risk of eating disorders (P < 0.05). In parallel, body shape perception was found to be marked with higher scores in NDD (72.0 ± 28.7) and PESD (71.5 ± 32.8) compared with SOC (64.2 ± 27.5) students (P < 0.05). Considering BSQ-34 classification, high concern (moderate and marked) for body shape were more in PESD (7.4 %) compared to NDD (5.2%) and SOC (1.9%) students (P < 0.05). The body size judgement via obtained by the FRS scale were generally correlated with BMI. The Body Mass Index levels were in normal range (Mean BMI: 21.9 ± 2.8 kg/m²) and generally consistent with FRS data. CONCLUSIONS: Tendency to the abnormal eating behavior and substantial body shape perception were higher in PESD students who have more concern on body shape and were not well-educated about nutrition. In conclusion, substantial concern on physical appearance might affect eating behavior disorders in PESD students.

Effectiveness of Cymbopogon citratus L. Essential Oil to Inhibit the Growth of Some Filamentous Fungi and Yeasts

Reyhan Irkin,Mihriban Korukluoglu 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.1

Lemon grass (Cymbopogon citratus L.) oil has been known as having therapeutic and antibacterial properties, and its antifungal activity is currently the subject of renewed interest. This study aimed to verify the effectivenesses of C. citratus essential oil to inhibit the growth/survival of some fungi (Alternaria alternata, Aspergillus niger, Fusarium oxysporum, and Penicillium roquefortii) and yeasts (Candida albicans, Candida oleophila, Hansenula anomala, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Saccharomyces uvarum, and Metschnikowia fructicola). C. citratus essential oil showed effectiveness in inhibiting the growth of all fungi by disc diffusion and broth dilution bioassay. Minimum inhibitory and minimum fungicidal concentrations between 0.062 and 20 μL/mL were determined. The Clinical and Laboratory Standards Institute agar-based method was also applied for A. niger and C. albicans. Data show the strong antifungal properties of lemon grass oil (C. citratus) in vitro.

Effects of Various Inhibitors on β-galactosidase Purified from the Thermoacidophilic Alicyclobacillus acidocaldarius Subsp. Rittmannii Isolated from Antarctica

Reyhan Gul Guven,Alevcan Kaplan,Kemal Guven,Fatma Matpan,Mehmet Dogru 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.1

β-Galactosidase purified from the thermoacidophilic Alicyclobacillus acidocaldarius subsp. rittmannii isolated from Antarctica is a member of the GH42 family. The enzyme was not effected by various concentrations of its reaction product glucose, but was greatly inhibited by the other reaction product galactose using both substrates,ONPG and lactose. Linewever-Burk plot analysis derived from both ONPG and lactose hydrolysis results showed that galactose is a mixed-type inhibitor of the purified β-galactosidase. The enzyme was slightly activated by Mg^(2+)(13% at 20 mM), while inhibited at higher concentrations of Ca^(+2) (33% at 10 mM), Zn^(+2) (86% at 8 mM) and Cu^(+2)(87% at 4 mM). The enzyme activity was not significantly altered by the metal ion chelators EDTA and 1,10-phenanthroline up to 20 mM, indicating that this enzyme is not a metalloenzyme. 2-Mercaptoethanol and DTT were found to enhance β-galactosidase activity, while p-chloromercuribenzoic acid (PCMB) completely inhibited enzymatic activity (97% at 1 mM; 99.7% at 2 mM), indicating at least one essential Cys residue modified by the reagents in the active site of β-galactosidase. Iodoacetamide and Nethylmaleimide had little effect on the β-galactosidase. Phenylmethylsulfonyl fluoride (PMSF) inhibited the enzyme strongly (19.8% at 1 mM; 71.9% at 10 mM), also showing the participation of serine for enzyme activity.

Determination of yolk:white ratio of egg using SDS-PAGE

Reyhan Selin Uysal,Esra Acar-Soykut,Ismail Hakki Boyaci 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.2

The aim of present study was to determine liquid egg adulteration based on yolk:white ratio of egg using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by measuring Brix. To construct a calibration model, liquid egg samples were prepared by mixing egg yolk and white (yolk:white) at different concentrations. A high coefficient of determination value (R2 = 0.992) was obtained. Limit of detection and limit of quantification were estimated as 62 g/kg and 187 g/kg. The accuracy of the model was tested using eleven LWE samples, and the yolk ratios of 90.9% of these samples were predicted successfully. Liquid whole egg (LWE) containing additional egg white up to 30% was also predicted with a low relative error of less than 10%. Yolk:white ratio of LWE samples and authentication of the components of LWE (containing extra white or water) can be determined using proposed method.

First record of Aphelinus paramali Zehavi and Rosen 1989 (Hymenoptera, Aphelinidae), parasitoid of Aphis pomi de Geer (Hemiptera, Aphididae) in Iran, and its phylogenetic position based on sequence data of ITS2 and COI genes

Reyhane DARSOUEI,Javad Karimi,Mehdi Modarres Awal 한국곤충학회 2011 Entomological Research Vol.41 No.5

The occurrence of Aphelinus paramali (Zehavi & Rosen) (Hym., Aphelinidae) was evidenced from North east Iran, in association with Aphis pomi (de Geer). This species is reported from Iran for the first time, and A. pomi is introduced as a new host for this parasitoid. Detailed morphological characters were studied with scanning electron microscopy (SEM) photographs. Also, sequences of ribosomal internal transcribed spacer 2 (ITS2) and cytochrome oxidase subunit I (COI) genes were used for determining species boundaries and comparing with other Aphelinus species. Different results were obtained in phylogenetic analysis of these two regions. Analysis of COI gene supported the closer relationship of this species with Aphelinus abdominalis. This is the first data about comprehensive characterization of a parasitic wasp using morphological characters, SEM and two-locus information from Iran.

Pyelo-cystic Reflux in F-18 FDG PET Scan Due to Ureteral Obstruction

Mehmet Reyhan 대한핵의학회 2013 핵의학 분자영상 Vol.47 No.3

Effectiveness of Cymbopogon citratus L. Essential Oil to Inhibit the Growth of Some Filamentous Fungi and Yeasts

Irkin, Reyhan,Korukluoglu, Mihriban The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.1

Lemon grass (Cymbopogon citratus L.) oil has been known as having therapeutic and antibacterial properties, and its antifungal activity is currently the subject of renewed interest. This study aimed to verify the effectivenesses of C. citratus essential oil to inhibit the growth/survival of some fungi (Alternaria alternata, Aspergillus niger, Fusarium oxysporum, and Penicillium roquefortii) and yeasts (Candida albicans, Candida oleophila, Hansenula anomala, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Saccharomyces uvarum, and Metschnikowia fructicola). C. citratus essential oil showed effectiveness in inhibiting the growth of all fungi by disc diffusion and broth dilution bioassay. Minimum inhibitory and minimum fungicidal concentrations between 0.062 and $20\;{\mu}L/mL$ were determined. The Clinical and Laboratory Standards Institute agar-based method was also applied for A. niger and C. albicans. Data show the strong antifungal properties of lemon grass oil (C. citratus) in vitro.