http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Raziallah Jafari Joozani (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Koolivand, Davoud,Bashir, Nemat Sokhandan,Behjatnia, Seyed Aliakbar,Joozani, Raziallah Jafari The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.5
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-${\beta}$-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting. The expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonal antibodies and application for the virus detection.
-
Davoud Koolivand,Nemat Sokhandan Bashir,Seyed Aliakbar Behjatnia,Raziallah Jafari Joozani 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.5
The genomic region of Grapevine fanleaf virus (GFLV)encoding the movement protein (MP) was clonedinto pET21a and transformed into Escherichia colistrain BL21 (DE3) to express the protein. Inductionwas made with a wide range of isopropyl-β-D-thiogalactopyranoside(IPTG) concentrations (1, 1.5, and2 mM) each for duration of 4, 6, or 16 h. However,the highest expression level was achieved with 1 mMIPTG for 4 h. Identity of the expressed protein wasconfirmed by sodium dodecyl sulphate polyacrylamidegel electrophoresis (SDS-PAGE) followed by Westernblotting. The expressed 41 kDa protein was purifiedunder denaturing condition by affinity chromatography,reconfirmed by Western blotting and platetrappedantigen enzyme-linked immunosorbent assay(PTA-ELISA) before being used as a recombinant antigento raise polyclonal antibodies in rabbits. Purifiedanti-GFLV MP immunoglobulines (IgGs) and conjugatedIgGs detected the expressed MP and GFLV virionsin infected grapevines when used in PTA-ELISA,double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonalantibodies and application forthe virus detection.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
