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Isolation and Characterization of Glycolate Oxidase Gene from Panax ginseng C. A. Meyer
Shohana Parvin,Rama Krishna Pulla,Yu-Jin Kim,Gayathri Sathiyaraj,Seok-kyu Jung,Altanzul Khorolragchaa,Jun Gyo In,Deok-Chun Yang 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.4
The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.
Isolation and Characterization of Cinnamoyl-CoA Reductase Gene from Panax ginseng C. A. Meyer
Shohana Parvin,Rama Krishna Pulla,Ju-Sun Shim,Yu-Jin Kim,Dea-Yeoung Jung,Se-Hwa Kim,Deok-Chun Yang 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3
Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specially dedicated to monolignol biosynthesis. A cDNA clones encoding CCR have been isolated from Panax ginseng C.A. Meyer and its expression was investigated in response to abiotic stresses. The cDNA, designated PgCCR which is 865 nucleotides long and has an open reading frame of 590 bp with a deduced amino acid sequence of 176 residues. The PgCCR encoded protein possesses substantial homology with CCRs isolated and cloned from other sources; the highest identity (51.8%) was observed with CCR from Tomato (Lycopersicon esculentum). Under various stress conditions, expression patterns of the PgCCR were highly induced in adventitious and hairy roots by several abiotic stresses. These results indicated that PgCCR plays protective role against diverse environmental stresses.
Isolation and Characterization of Glycolate Oxidase Gene from Panax ginseng C. A. Meyer
Parvin, Shohana,Pulla, Rama Krishna,Kim, Yu-Jin,Sathiyaraj, Gayathri,Jung, Seok-Kyu,Khorolragchaa, Altanzul,In, Jun-Gyo,Yang, Deok-Chun The Korean Society of Ginseng 2009 Journal of Ginseng Research Vol.33 No.4
The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.