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Pham, Quang Nghia,Trinh, Kieu The Loan,Tran, Nguyen Khoi Song,Park, Tae-Sik,Lee, Nae Yoon The Royal Society of Chemistry 2018 The Analyst Vol.143 No.23
<P>We fabricate a three-dimensional (3D) microdevice operated with minimal peripheral accessories, including a portable pump for semi-automated sample delivery and a single heater for temperature control, for performing reverse transcription polymerase chain reaction (RT-PCR) integrated with a downstream fluorescence detection module for semi-quantitative assessment of gene expression. The microdevice was fabricated by wrapping a polytetrafluoroethylene (PTFE) tube around a pre-designed polycarbonate mold to create a seamless microchannel for both the reverse transcription (RT) of RNA and the amplification of complementary DNA. In addition, a silicone tube, which underwent a two-step surface modification mediated by polyethyleneimine and glutaraldehyde coating, was connected at the outlet to capture amplicons downstream of the PTFE tube for on-site fluorescence detection. This fabrication method enabled continuous-flow RT-PCR (CF RT-PCR) using the 3D CF RT-PCR microdevice as a reactor, a single heater for the temperature control of both RT and PCR processes, and a disposable plastic syringe for semi-automated sample delivery. The microdevice was successfully implemented for the identification of the β-actin gene, a constitutively expressed gene in all cells, and the sphingosine-1-phosphate lyase 1 gene, a potential pharmacological target gene in the diagnosis of cancer, diabetes, and atherosclerosis. This portable integrated microdevice offers a potential approach towards preliminary studies of gene expression and identification of RNA viruses.</P>
Ha-Linh Quach,Thai Quang Pham,Ngoc-Anh Hoang,Dinh Cong Phung,Viet-Cuong Nguyen,Son Hong Le,Thanh Cong Le,Dang Hai Le,Anh Duc Dang,Duong Nhu Tran,Nghia Duy Ngu,Florian Vogt,Cong-Khanh Nguyen 대한의료정보학회 2022 Healthcare Informatics Research Vol.28 No.4
Objectives: Online misinformation has reached unprecedented levels during the coronavirus disease 2019 (COVID-19) pandemic. This study analyzed the magnitude and sentiment dynamics of misinformation and unverified information about public health interventions during a COVID-19 outbreak in Da Nang, Vietnam, between July and September 2020. Methods: We analyzed user-generated online information about five public health interventions during the Da Nang outbreak. We compared the volume, source, sentiment polarity, and engagements of online posts before, during, and after the outbreak using negative binomial and logistic regression, and assessed the content validity of the 500 most influential posts. Results: Most of the 54,528 online posts included were generated during the outbreak (n = 46,035; 84.42%) and by online newspapers (n = 32,034; 58.75%). Among the 500 most influential posts, 316 (63.20%) contained genuine information, 10 (2.00%) contained misinformation, 152 (30.40%) were non-factual opinions, and 22 (4.40%) contained unverifiable information. All misinformation posts were made during the outbreak, mostly on social media, and were predominantly negative. Higher levels of engagement were observed for information that was unverifiable (incidence relative risk [IRR] = 2.83; 95% confidence interval [CI], 1.33–0.62), posted during the outbreak (before: IRR = 0.15; 95% CI, 0.07–0.35; after: IRR = 0.46; 95% CI, 0.34-0.63), and with negative sentiment (IRR = 1.84; 95% CI, 1.23–2.75). Negatively toned posts were more likely to be misinformation (odds ratio [OR] = 9.59; 95% CI, 1.20–76.70) or unverified (OR = 5.03; 95% CI, 1.66–15.24). Conclusions: Misinformation and unverified information during the outbreak showed clustering, with social media being particularly affected. This indepth assessment demonstrates the value of analyzing online “infodemics” to inform public health responses.
Oxidize Graphene by UV-Ozone Treatment in Vacuum Chamber
Dinh, S. Thach,Pham, T. H. Nhung,Nguyen, T. T. Hien,Dinh, N. T. Nghia,Vu, V. Quang,Hoang, T. K. Dung,Duong, D. Loc American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.8
<P>As changing graphene properties is vital to promote it as electronic device, we developed graphene modified method with epoxy group by two simple methods: ultraviolet irradiation with various partial oxygen pressures and ultraviolet irradiation under rich oxygen condition with various temperatures. By Hall measurement, the electric mobility in graphene was decreased with increasing temperature during UV-ozone treatment at 50 degrees C, 80 degrees C, 100 degrees C and 120 degrees C. At low partial oxygen pressure (less than 160 Torr) the oxidation degree is not significant. Additionally, Raman spectroscopy showed the disrupted pi-pi structure in graphene. Based on the above identification we supposed that epoxy group might establish on graphene surface.</P>
Aerodynamic Performance of an Annular Combustion Chamber Cooling with Swirler Design
Cong-Truong Dinh,Xuan-Truong Le,Trong-Nghia Hoang,Quang-Anh Pham 한국유체기계학회 2021 International journal of fluid machinery and syste Vol.14 No.1
Gas turbines play a crucial role in the aviation industry as they are primary sources of power for most aircraft. The combustion chamber is one of the three essential part of jet engines, together with compressor and turbine. Energy is generated when fuel is burned in the combustor. In the primary zone, the recirculation flow is of great importance to aerodynamic performance. Swirlers, fitted in the dome around the fuel injector, can alter the behavior of the recirculation flow and thus, impact combustion performance. The vortex behind a swirler can be easily controlled by changing the swirl angle. In addition, changing the vortex angle leads to a difference in mixing between fuel and air. This paper investigates the effects of swirler design, based on the swirl angle, on aerodynamic performance of an annular combustion chamber in cooling condition using three-dimensional Reynolds-averaged Navier-Stokes equations with the SST turbulence model.