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Anti-bacterial and Anti-viral Activity of Extracts from Paeonia lactiflora Roots
부경환,Doseung Lee,Jin-Kyu Woo,Seung Hee Ko,Eun-Hyoung Jeong,Quanchun Hong,Key Zung Riu,Dong-Sun Lee 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
Methanol extracts of Paeonia lactiflora roots (Radix Paeaniae Lactiflora) had antibacterial activity against Bacillus subtilis. These extracts generated superoxide radicals in the B. subtilis lysate. However, the radicals were strongly detoxified by the addition of superoxide dismutase, an indication that superoxide radicals are important in the antibacterial actions of the extracts. The root extracts also showed a pivotal inhibition role in trafficking of viral glycoprotein in virusinfected baby hamster kidney (BHK) cells. The methanol extract effectively inhibited syncytium formation in a concentration-dependent, and it did not affect glycoprotein synthesis. These results suggest that oxygen radical affect the transport process of viral glycoprotein without its synthesis.
Trafficking Inhibition of Bruceanol B as a Radical-Producing Antibiotic
Dong-Sun Lee,Doseung Lee,부경환,Jin-Kyu Woo,Quanchun Hong,Somi K. Cho,Se Pill Park,Key Zung Riu 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Bruceanol B, isolated from a soil microbe strain DS4, generated oxygen radicals in Bacillus subtilis lysates. Bruceanol B inhibited the trafficking of viral glycoprotein in virus-infected baby hamster kidney (BHK) cells. Bruceanol B also effectively inhibited syncytium formation in a dosedependent manner. However, glycoprotein synthesis was not affected by the compound. Results indicate that bruceanol B generating oxygen radical has inhibitory activity in the trafficking of viral glycoprotein of BHK cell.
Distribution and Biosynthesis of 20-Hydroxyecdysone in Plants of <i>Achyranthes japonica</i> Nakai
BOO, Kyung Hwan,LEE, Doseung,JEON, Gyeong Lyong,KO, Seung Hee,CHO, Somi K.,KIM, Jae Hoon,PARK, Se Pill,HONG, Quanchun,LEE, Sang-Han,LEE, Dong-Sun,RIU, Key Zung Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, biotechnology, and biochemistry Vol.74 No.11
<P>There is increasing interest in phytoecdysteroids (PEs) because of their potential role in plant defense against insects. To understand the mechanism regulating their levels in plants, the fluctuation, distribution, and biosynthesis of PE 20-hydroxyecdysone (20E) examined in <I>Achyranthes japonica</I>. The total amount of 20E per individual plant initially remained at a constant level, and increased markedly after the first leaf pair (LP) stage, while the concentration of 20E in a given plant decreased rapidly during vegetative growth. In addition, the incorporation of [2-<SUP>14</SUP>C]-mevalonic acid into 20E did not differ significantly depending on plant organs and developmental stages, suggesting that biosynthesis of 20E is not restricted to particular organs or growth stages.</P>