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RISS 인기검색어
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- 저자 : Qu-Fei Ling (검색결과 3 건)
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Huang, Fei,Zhao, Feng,Liang, Li-Ping,Zhou, Mei,Qu, Zhi-Ling,Cao, Yan-Zhen,Lin, Chen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17
Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.
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Gu, Mei-Ling,Yuan, Cai-Jun,Liu, Xiao-Mei,Zhou, Yi-Chao,Di, Shu-Huan,Sun, Fei-Fei,Qu, Quan-Ying Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13
Purpose: To research the association between pre-treatment elevated platelet count and clinicopathologic characteristics in breast cancer (BC), as well as explore the relationship between pre-treatment elevated platelet count and HER2 status and prognosis of BC patients. Materials and Methods: A retrospective cohort of BC patients who were newly diagnosed or treated by surgery only and had pathological detection results and platelet values in the Department of Oncology, the First Affiliated Hospital of Liaoning Medical College were enrolled from 1/1/2008 until 31/12/2009, and followed up until 31/12/2014. Age, thrombocyte parameters before chemotherapy and/or radiotherapy, immunohistochemical (IHM) indexes, and regional lymph node (LN) involvement and progression-free survival (PFS) were recorded. Results: A total of 447 eligible subjects were included in this research. As we analyzed, for HER2, positive and negative, the incidence rates of elevated platelet count were 25.8% and 14.7% (P<0.05). In the Cox proportional hazards model both variables were independent risk factors for BC (for HER2, OR, 0.592, 95% confidence interval, CI, 0.355 to 0.985, P=0.044;f or PLT, OR, 0.998, 95% CI, 0.996 to 1.000, P=0.042). For ER, PR, Ki67 and LN involvement, the differences were not statistically significant (P>0.05). Conclusions: In this research, pre-treatment elevated level of platelet count demostrated a significantrelationship with HER2 amplification/overexpression, and both variables significantly influenced the prognosis of BC. However, elevated platelet count did not exhibit any association with ER, PR, Ki67 and LN involvement.
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Qian Zhang,Meng-Meng Shang,Qu-Fei Ling,Xiao-Ping Wu,Chun-Yu Liu 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.8
This study investigates the hepatoprotectiveeffects and the potential therapeutic mechanisms of loach(Misgurnus anguillicaudatus) lyophilized powder (MLP)on dimethylnitrosamine (DMN) induced liver fibrosis inrats. After treatment with MLP (50, 100, 200 mg/kg),alanine aminotransferase (ALT), aspartate aminotransferase(AST), albumin (Alb), total protein (TP) andhydroxyproline (Hyp) levels were detected, to assess thedestruction of hepatocytes and the extent of liver fibrosis. Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), hyaluronic acid (HA), Laminin (LN),procollagen type-III (PC-III), collagen type-IV (C-IV), andtransforming growth factor-b1 (TGF-b1) contents in serumwere all tested using ELISA kits. Alpha-smooth muscleactin (a-SMA) and the tissue inhibitor of metalloproteinase-1 (TIMP-1) protein contents and distribution wereevaluated using western blot and immunohistochemicalanalysis. MLP significantly decreased the serum concentrationsof ALT, AST, Hyp, HA, LN, PC-III, C-IV, MMP-2,TIMP-1, a-SMA and TGF-b1, while increasing the contentsof Alb and MMP-9. No significant changes on TP serum concentrations were observed. These results suggest thatMLP has anti-hepatic fibrosis effects and its mechanism maybe associated with the attenuation of extracellular matrix(ECM) synthesis, the acceleration of ECM degradation,inhibition of hepatic stellate cells (HSCs) activation andTGF-b1 expression.
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