http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Qu Wanming (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
MicroRNA-5582-3p alleviates the progression of osteosarcoma via targeting FZD4
Qu Wanming,Zhou Hongbin 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.2
Background Dysfunction of microRNAs (miRNAs) is a common characteristic during cancer progression. The suppressive function of miR-5582-3p in cancers has been emerged in recent studies, however, the clinical value and novel targets of miR-5582-3p in osteosarcoma (OS) have not been identifi ed. Objective Investigate the function and underlying mechanism of miR-5582-3p in OS. Results miR-5582-3p expression was signifi cantly reduced in OS tissues and cells. Lower level of miR-5582-3p had closed relationship with the advanced development and poor 5-year overall survival of OS patients. Transfection of miR-5582-3p in OS cells obviously inhibited the cell proliferation, invasion and colony formation. Highly expressed miR-5582-3p also delayed the cell cycle progression and promoted cell apoptosis. Furthermore, FZD4 was validated as a target of miR-5582-3p. miR- 5582-3p interacted with the 3′-untranslated regions (3′-UTR) of FZD4 and reduced FZD4 expression in OS cells. Expression of miR-5582-3p was inversely correlated with that of FZD4 in OS tissues. Transfection of FZD4 rescued the suppressed OS cell proliferation, attenuated miR-5582-3p induced cell apoptosis and invasion. Conclusions Our fi ndings uncovered the novel anti-cancer potency of miR-5582-3p in OS via repressing FZD4, suggesting the possibility of miR-5582-3p as a therapeutic target of OS.
-
Yang Zhuqing,Bao Linbin,Song Wanming,Zhao Xianghui,Liang Huan,Yu Mingjin,Qu Mingren 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2
Objective: The aim of this study was to investigate the impact of dietary nicotinic acid (NA) on apparent nutrient digestibility, rumen fermentation, and rumen microbiota in uncastrated Xiangzhong black cattle. Methods: Twenty-one uncastrated Xiangzhong black cattle (385.08±15.20 kg) aged 1.5 years were randomly assigned to the control group (CL, 0 mg/kg NA in concentrate diet), NA1 group (800 mg/kg NA in concentrate diet) and NA2 group (1,200 mg/kg NA in concentrate diet). All animals were fed a 60% concentrate diet and 40% dried rice straw for a 120-day feeding experiment. Results: Supplemental NA not only enhanced the apparent nutrient digestibility of acid detergent fiber (p<0.01), but also elevated the rumen acetate and total volatile fatty acid concentrations (p<0.05). 16S rRNA gene sequencing analysis of rumen microbiota revealed that dietary NA changed the diversity of rumen microbiota (p<0.05) and the abundance of bacterial taxa in the rumen. The relative abundances of eight Erysipelotrichales taxa, five Ruminococcaceae taxa, and five Sphaerochaetales taxa were decreased by dietary NA (p< 0.05). However, the relative abundances of two taxa belonging to Roseburia faecis were increased by supplemental 800 mg/kg NA, and the abundances of seven Prevotella taxa, three Paraprevotellaceae taxa, three Bifidobacteriaceae taxa, and two operational taxonomic units annotated to Fibrobacter succinogenes were increased by 1,200 mg/kg NA in diets. Furthermore, the correlation analysis found significant correlations between the concentrations of volatile fatty acids in the rumen and the abundances of bacterial taxa, especially Prevotella. Conclusion: The results from this study suggest that dietary NA plays an important role in regulating apparent digestibility of acid detergent fiber, acetate, total volatile fatty acid concentrations, and the composition of rumen microbiota.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
