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        Analysis of Fatty Acids, Aliphatic Esters, and In Vitro Studies of Antioxidant and Antimicrobial Activities for Recineckea carnea and Tupistra chinensis from the Guizhou Province

        Qisi Lin,Miao Wang,Jinghua Li,Wanping Shi,Hui Wang,Chunjie Zhao 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.2

        Recineckea carnea and Tupistra chinensis collected from the Guizhou province (China) were evaluated in this study. Petroleum ether fractions from the two herbs were subjected to gas chromatography–mass spectrometry analysis; 10 species, which were fatty acids or aliphatic esters, were identified. The antimicrobial activities of a variety of extracts were evaluated against four microorganisms. The methanol extract (ME), chloroform fraction, and ethyl acetate fraction from T. chinensis exhibited antimicrobial activities comparable to standard antibiotics, whereas none of the investigated extracts from R. carnea demonstrated any antimicrobial activities. The antioxidant potential was evaluated in vitro using ferricreducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical method. The FRAP value of the ME from T. chinensis (4.19 – 0.088 mmol/g) was found to be significantly higher than the analogous extract from R. carnea (2.39 – 0.092 mmol/g); the EC50 of the ME from R. carnea (0.32 – 0.011 mg/mL) was found to be significantly higher than that of T. chinensis (0.30 – 0.015 mg/mL). Total phenolic content was estimated by the Folin–Ciocalteu’s colorimetric method. A positive correlation was found between total phenolic content and antioxidant activities (FRAP value and the reciprocal of EC50). The results suggested that the phenolic compounds contributed significantly to the antioxidant capacity of R. carnea and T. chinensis.

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        Contribution of Phenolics and Essential Oils to the Antioxidant and Antimicrobial Properties of Disporopsis pernyi (Hua) Diels

        Qisi Lin,Ling Zhang,Dongzhi Yang,Zhao Chunjie 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.6

        This study describes the characterization of essential oil and phenolic compounds in Disporopsis pernyi (Hua) Diels. The essential oil was analyzed by gas chromatography-mass spectroscopy (GC-MS) and twelve volatile bioactive compounds of D. pernyi were identified. Polyphenols were identified by reverse-phase high-performance liquid chromatography (RP-HPLC) coupled to electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) and quantified by ultrahigh-performance liquid chromatography (UPLC). A total of 4 polyphenolic compounds found in extract were identified as rutin, luteolin, quercetin, and betulinic acid. All obtained extracts and the 4 polyphenolic compounds were evaluated for their antimicrobial and antioxidant properties. The results suggested that the phenolic compounds contributed significantly to the antioxidant and antimicrobial activities of the plant.

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