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- 저자 : Qingchun Gao (검색결과 3 건)
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Gao Ruonan,Li Qingchun,Qiu Meiyu,Xie Su,Sun Xiaomei,Huang Tao 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.9
Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC] = 0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.
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Miao Shui,Weidong Zheng,Jie Shu,Qingchun Wang,Shan Gao,Dan Xu,Liangliang Cheng,Lin Feng,Yuanlong Ren 한국물리학회 2013 Current Applied Physics Vol.13 No.3
Lithium-ion battery cathode material Li1þxV3O8 is synthesized by a citric acid/tartaric acid assisted sol egel method and sintered at 350 C, 450 C and 550 C for 3 h for the formation of Li1þxV3O8 phase. The synthesized samples were fully characterized by FTIR, TG/DTA, XRD, SEM, EIS and chargeedischarge tests. Li1þxV3O8 material synthesized by tartaric acid assisted route and sintered at 450 C for 3 h shows best electro-chemical performance. It shows a high initial capacity of 249 mAh g1 and still reserves a discharge capacity of 260 mAh g1 after 50 cycles. Moreover, in the case of tartaric assisted products, no capacity decadence is observed in 50 cycles. XRD together with TG/DTA measurements reveal that compared with citric acid assisted products, the adoption of tartaric acid as chelating agent effectively lowers the crystallization temperature of amorphous Li1þxV3O8. Therefore, precursors obtained by tartaric acid route calcinated at 450 C for 3 h exhibit lower crystallinity and smaller grain size, which contributes to the better electrochemical performance of the cathode electrodes. From EIS measurements, the bulk resistance is reduced, which favors the intercalation and de-intercalation of lithium ions while cycling.
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Xuelong Li,Heng Du,Jia Li,Xianliang Li,Qingchun Gao,Xiangyan Chen 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.4
Background and Purpose By measuring a newly defined parameter, the carotid–cerebral pulse wave velocity (ccPWV), this study aimed to determine the association of intracranial artery calcification (IAC) with arterial stiffness as reflected by the pulse wave velocity between the carotid and middle cerebral arteries using transcranial Doppler sonography in patients with acute stroke. Methods We recruited 146 patients with ischemic stroke from our stroke center. Computed tomography of the head was used to assess the presence and severity of IAC. Arterial stiffness was evaluated using ccPWV. Data are presented as quartiles of ccPWV. A multivariable logistic regression model was used to assess the independent relationship between ccPWV and IAC. Results The IAC prevalence increased with the ccPWV quartile, being 54%, 76%, 83%, and 89% for quartiles 1, 2, 3, and 4, respectively (p<0.001) as did IAC scores, with median [interquartile range] values of 0 [0–2], 3 [2–4], 4 [2–5], and 5 [4–6], respectively (p<0.001). After additionally adjusting for age and hypertension, a significant correlation was only found between quartiles 3 and 4 of ccPWV and IAC scores. The odds ratio (95% confidence interval) for the IAC scores was 1.78 (1.28–2.50) (p=0.001) in quartile 4 of ccPWV and 1.45 (1.07–1.95) (p=0.015) in quartile 3 compared with quartile 1. Conclusions We found that in patients with acute ischemic stroke, ccPWV was positively related to the degree of IAC. Future longitudinal cohort studies may help to identify the potential role of IAC in the progression of cerebral arterial stiffness.
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