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Gene expression and promoter methylation of porcine uncoupling protein 3 gene
Ruiyi Lin,Weimin Lin,Qiaohui Chen,Jianchao Huo,Yuping Hu,Junxiao Ye,Jingya Xu,Tianfang Xiao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.2
Objective: Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods: Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5′-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results: Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion: Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.
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Yibo Yang,Baixi Chen,Weizhen Zeng,Yanjun Li,Qiaohui Chen,Wenying Guo,Hengchang Wang,Yingqin Chen 한국콘크리트학회 2021 International Journal of Concrete Structures and M Vol.15 No.5
To reduce the cost of lightweight concrete (LWC) partition panels and to address recycling concrete waste, this work utilized completely recycled fine aggregate (CRFA) to replace the natural fine aggregate and ceramsite in the preparation of LWC and LWC partition panels. To this end, an autoclave-free curing process and an air-entraining agent were used to prepare the CRFA-LWC. The workability, compressive strength, drying shrinkage, and pore structure of the CRFA-LWC and the performance of the CRFA-LWC partition panels were then investigated. The results show that the optimal ratio of the CRFA to the cement is 2.2 for the lightweight concrete, and the optimal panel cross section is a rounded rectangular one. All the pores in the CRFA-LWC have a diameter of smaller than 0.17 mm, and the diameter of 89% of them is less than 0.05 mm. In order to satisfy the drying shrinkage requirements stipulated by Chinese code JC/T 169-2016, the CRFA-LWC should be cured for at least 10 days. The economic analysis concludes that the material cost of CRFA-LWC is 40% lower than that of the autoclaved ceramsite concrete. In addition, utilizing CRFA in lightweight concrete can ease the shortage of natural aggregate.
ScienceON
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