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      • Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

        Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

        Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.

      • The atomization characteristics of the surrogate fuel of diesel from indirect coal liquefaction at engine conditions

        ( Zhong Huang ),( Tingting Zhang ),( Dehao Ju ),( Xinqi Qiao ),( Jin Xiao ),( Zhen Huang ) 한국액체미립화학회 2017 한국액체미립화학회 학술강연회 논문집 Vol.2017 No.-

        The fuel spray behavior has a significant impact on the performance and emission characteristics of the compression ignition engine. It controls the fuel air mixing, which in turns is critical for the combustion process. The differences in the physical properties of convention diesel and the diesel from coal indirect liquefaction (DICL) are expected to result in differences in their spray characteristics, thus, in the performance and emission characteristics of the engine. The aim of this work is to analyze the atomization characteristics of the DICL and compare the global and local flow characteristics of the DICL with the NO.2 diesel (D2) at engine conditions. A surrogate fuel of the DICL (a mixture of 72.4% n-dodecane and 27.6% methylcyclohexane by mass) was built according to its components to simulate the atomization characteristics of the DICL in the non-reacting high temperature and high pressure environment. The implicit Large Eddy Simulation (LES) was used together with the Lagrangian particle tracking approach for the liquid phase in the present study. The simulation results show that the liquid penetration of the DICL is shorter than that of theD2, while there is no significant difference in the vapor penetration of the two fuels. In addition, the gas-phase axial velocity of the DICL along the spray center line is slightly larger than that of D2 in upstream of the spray. Moreover, the SMD of the DICL is larger than that of D2. The studies on the effects of the injection pressure on the atomization characteristics of the DICL were also carried out. The results imply that the injection pressure of the DICL is not required as large as the injection pressure for D2 to generate the same spray quality. This study is helpful to understand the differences in the atomization characteristics between DICL and D2.

      • SCOPUSSCIEKCI등재

        Giant Ganglioneuroma of Thoracic Spine : A Case Report and Review of Literature

        Huang, Yong,Liu, Lidi,Li, Qiao,Zhang, Shaokun The Korean Neurosurgical Society 2017 Journal of Korean neurosurgical society Vol.60 No.3

        Ganglioneuroma (GN) is a rare benign tumor of neural crest origin usually found in the abdomen, but may occasionally present at uncommon sites including the cervical, lumbar, or sacral spine. However, GNs of thoracic spine are extremely rare. In this report, we describe a 12-year-old girl with giant GN in the thoracic spine, who underwent successful resection (T1-4 level) of the tumor. Histopathological examination confirmed the diagnosis. GN should be considered in the differential diagnosis of any paraspinal mass. A high index of suspicion and correlation of clinico-radiological findings is necessary in differentiating a large benign tumor from a malignant growth. Complete surgical excision is the treatment of choice; however tumor size and location need to be considered for the surgical approach (one-step or multiple surgeries). Close follow-up after surgery is mandatory.

      • SCISCIESCOPUS

        Hydrothermal synthesize and photocatalytic ability of a new silver vanadate semiconductor K<sub>2/5</sub>Ag<sub>3/5</sub>VO<sub>3</sub> with stable α-AgVO<sub>3</sub> structure

        Qiao, Xuebin,Huang, Yanlin,Cheng, Han,Seo, Hyo Jin Elsevier 2016 Materials letters Vol.164 No.-

        <P><B>Abstract</B></P> <P>A silver vanadate semiconductor K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> with the stable α-AgVO<SUB>3</SUB> structure has been prepared via the hydrothermal method. The structural refinements were completed. Similar to the well-known β-AgVO<SUB>3</SUB>, K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> also keeps one-dimensional crystallization habit with a high ratio of length to diameter. The surface was characterized by the SEM, TEM, energy dispersive spectrometer (EDS) spectrum and nitrogen adsorption–desorption isotherms. The UV–vis absorption spectrum shows that K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> nanowire has narrow band energy (2.02eV) characterized by indirect allowed electronic transition. K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> nanowires present a very good dispersion in water. Photocatalysis was tested by the photo-degradation of methylene blue (MB) solution. The textile structure of the nanowires makes recovery possible by filtering after photocatalytic reactions<B>.</B> K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> nanowires show effective photocatalytic ability under visible-light irradiation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> semiconductor was developed by the hydrothermal method. </LI> <LI> K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> presents stable α-AgVO<SUB>3</SUB> structure with narrow band (2.02eV). </LI> <LI> It keeps one-dimensional crystallization with a high ratio of length to diameter. </LI> <LI> The nanowires present very good dispersion in water and can be well recycled. </LI> <LI> K<SUB>2/5</SUB>Ag<SUB>3/5</SUB>VO<SUB>3</SUB> has effective photocatalytic ability under visible-light irradiation. </LI> </UL> </P>

      • SCISCIESCOPUS
      • SCISCIESCOPUS
      • Human Papillomavirus Infection and Prognostic Predictors in Patients with Oropharyngeal Squamous Cell Carcinoma

        Huang, Hui,Zhang, Bin,Chen, Wen,Zhou, Shuang-Mei,Zhang, Yong-Xia,Gao, Li,Xu, Zhen-Gang,Qiao, You-Lin,Tang, Ping-Zhang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

        This study focused on infection rates and subtypes of human papillomavirus (HPV) in patients with oropharyngeal squamous cell carcinoma (OSCC), and the relationship between HPV status and prognosis of the disease. We evaluated sixty-six OSCC patients who met the enrollment criteria during the period from January 1999 to December 2009. The presence or absence of oncogenic HPV types in tumors was determined using the SPF10 LiPA25 assay. Overall survival (OS) and disease specific survival (DSS) for HPV positive and HPV negative patients were estimated using Kaplan-Meier analysis. The Cox regression model was applied for multivariate analysis. HPV-DNA was detected in 11(16.7%) of all specimens. Among them, 7 were type HPV-16, while other types were HPV-16/11, HPV-35, HPV-58/52, and HPV-33/52/54. Patients with HPV positive tumors were more likely to be female, non-smokers and non-drinkers (p=0.002, 0.001 and 0.001, respectively). After a median follow-up of 24.5 months, patients with HPV positive tumors had significantly better overall survival (HR=0.106[95%CI=0.014-0.787], p=0.016,) and disease specific survival (HR=0.121[95%CI=0.016-0.906], p=0.030). Patients with HPV positive OSCC have significantly better prognosis than patients with HPV negative tumors. HPV infection is an independent prognostic factor.

      • SCIESCOPUSKCI등재

        Exogenous Spermidine Promoted Ca<SUP>2+</SUP> Absorption in Lettuce Roots and Reduced the Incidence of Tipburn

        Qiao Yang,Na Lu,Lei Wang,Xiao-qing Huang,Da-quan Yang,Jin Sun 한국원예학회 2018 원예과학기술지 Vol.36 No.5

        A physiological disorder, called tipburn, commonly occurs in lettuce (Lactuca sativa L.), particularly in greenhouse cultivation and in hot seasons, and reduces its marketability. Calcium (Ca) deficiency is the main cause of tipburn injury. In the present study, we investigated the effect of exogenous spermidine (Spd) on the occurrence of tipburn in hydroponically cultivated head lettuce (var. capitata L.) and elucidated the role of Ca ions (Ca<SUP>2+</SUP>) in its prevention with respect to absorption and transport. Ca deficiency in the nutrient solution resulted in reduced uptake of Ca<SUP>2+</SUP> in lettuce roots, thereby resulting in Ca deficiency in leaves, leading to leaf senescence and tipburn. An application of exogenous Spd promoted Ca<SUP>2+</SUP> uptake in lettuce roots under Ca deficiency and promoted Ca<SUP>2+</SUP> transportation to leaves, especially to inner leaves, thus delaying leaf senescence and reducing the incidence of tipburn. The results indicated that application of exogenous Spd is an effective method to decrease tipburn in lettuce caused by Ca deficiency.

      • SCIESCOPUSKCI등재

        Effects of Lactobacilli on the Performance, Diarrhea Incidence, VFA Concentration and Gastrointestinal Microbial Flora of Weaning Pigs

        Huang, Canghai,Qiao, Shiyan,Li, Defa,Piao, Xiangshu,Ren, Jiping Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3

        Two experiments were conducted to evaluate the effects of a complex Lactobacilli preparation on performance, resistance to E. coli infection and gut microbial flora of weaning pigs. In exp. 1, twelve pigs (7.65$\pm$1.10 kg BW), weaned at 28 d, were randomly allotted into 2 groups and placed in individual metabolic cages. During the first 7 d, one group of pigs was provided ad libitum access to water containing $10^5$ colony forming units (CFU) Lactobacilli per ml and the control group was provided tap water. The Lactobacilli preparation included Lactobacillus gasseri, L. reuteri, L. acidophilus and L. fermentum, which were isolated from the gastrointestinal (GI) tract mucosa of weaning pigs. On d 8, 20 ml of $10^8$ CFU/ml E. coli solution (serovars K99, K88 and 987P at the ratio of 1:1:1) was orally administered to each pig. Diarrhea scores and diarrhea incidence were recorded from d 7 to 14. On d 14, pigs were euthanized and digesta and mucosa from the stomach, duodenum, jejunum, ileum, cecum and colon were sampled using aseptic technique to determine microflora by culturing bacteria in selective medium. The results showed that Lactobacilli treatment significantly decreased E. coli and aerobe counts (p<0.01) but increased Lactobacilli and anaerobe counts (p<0.01) in digesta and mucosa of most sections of the GI tract. A 66 and 69.1% decrease in diarrhea index and diarrhea incidence, respectively, was observed in the Lactobacilli treated group. In exp. 2, Thirty-six crossbred Duroc$\times$Landrace$\times$Yorkshire piglets, weaned at 28$\pm$2 days, were selected and randomly allocated into 2 groups. There were 18 piglets in each group, 3 piglets in one pen and 6 replicates in each treatment with 3 pens of barrow and 3 pens of female piglet in each treatment. Piglets had ad libitum access to feed and water. The initial body weight of piglet was 7.65$\pm$1.09 kg. Dietary treatments included a non-medicated basal diet with Lactobacilli ($10^5$ CFU/g diet) or carbadox (60 mg/kg) as control. On d 21, six pigs per group (one pig per pen) were euthanized. Ileal digesta was collected to determine apparent amino acid digestibility. Microflora content was determined similarly to exp.1. The results showed that Lactobacilli treatment significantly improved average daily feed intake (ADFI) of pigs compared to carbadox (p<0.05) during the first 2 wks after weaning and average daily gain (ADG) and ADFI increased significantly (p<0.05) from d 8 to 14. Nitrogen and total phosphorus digestibility also increased (p<0.05). Bacterial counts were similar to exp. 1. The results indicate that the complex Lactobacilli preparation improved performance for 2 wks after weaning, enhanced resistance to E. coli infection, and improved microbial balance in the GI tract.

      • KCI등재

        Exogenous Spermidine Promoted Ca2+ Absorption in Lettuce Roots and Reduced the Incidence of Tipburn

        Qiao Yang,Na Lu,Lei Wang,Xiao-qing Huang,Da-quan Yang,Jin Sun 한국원예학회 2018 원예과학기술지 Vol.36 No.5

        A physiological disorder, called tipburn, commonly occurs in lettuce (Lactuca sativa L.), particularly in greenhouse cultivation and in hot seasons, and reduces its marketability. Calcium (Ca) deficiency is the main cause of tipburn injury. In the present study, we investigated the effect of exogenous spermidine (Spd) on the occurrence of tipburn in hydroponically cultivated head lettuce (var. capitata L.) and elucidated the role of Ca ions (Ca2+) in its prevention with respect to absorption and transport. Ca deficiency in the nutrient solution resulted in reduced uptake of Ca2+ in lettuce roots, thereby resulting in Ca deficiency in leaves, leading to leaf senescence and tipburn. An application of exogenous Spd promoted Ca2+ uptake in lettuce roots under Ca deficiency and promoted Ca2+ transportation to leaves, especially to inner leaves, thus delaying leaf senescence and reducing the incidence of tipburn. The results indicated that application of exogenous Spd is an effective method to decrease tipburn in lettuce caused by Ca deficiency.

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