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- 저자 : Qi-cong Peng (검색결과 2 건)
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Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion
Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.
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On Diagonal Loading for Robust Adaptive Beamforming Based on Worst-Case Performance Optimization
Jing-ran Lin,Qi-cong Peng,Huai-zong Shao 한국전자통신연구원 2007 ETRI Journal Vol.29 No.1
Robust adaptive beamforming based on worst-case performance optimization is investigated in this paper. It improves robustness against steering vector mismatches by the approach of diagonal loading. A closed-form solution to optimal loading is derived after some approximations. Besides reducing the computational complexity, it shows how different factors affect the optimal loading. Based on this solution, a performance analysis of the beamformer is carried out. As a consequence, approximated closed-form expressions of the source-of-interest power estimation and the output signalto- interference-plus-noise ratio are presented in order to predict its performance. Numerical examples show that the proposed closed-form expressions are very close to their actual values.
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