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Alok Kumar Varshney,Medicherla Krishna Mohan,Ambarish Sharan Vidyarthi,Vinod Kumar Nigam,Purnendu Ghosh 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
The production of manganese peroxidase (MnP)by the white-rot fungus Phanerochaete chrysosporiumNCIM 1197 was investigated by the screening andoptimization of the media constituents and physiologicalfactors. MnP production by the fungus was used as theresponse to screen the media constituents with statisticallyvalid Plackett-Burman (P-B) design. Response surfacemethodology (RSM) was applied to optimize the level ofscreened media constituents. Amongst the media constituentsscreened, glucose, maltose, ammonium chloride, and ureawere selected as the most important for MnP enhancement. A five-level Central Composite Design (CCD) was used inoptimizing the important media constituents for maximizingthe MnP production. The optimal medium composition formaximum MnP production was 13.88 mM of glucose,13.88 mM of maltose, 0.02 mM of ammonium chloride,and 0.02 mM of urea. The final experiment was conductedto validate the model, which was shown to produce70.20 U/mL of MnP with a predicted value of 66.49 U/mLon the 8th day of incubation.