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Puji Lestari,최우혁 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Stretchable and self-healing properties are highly required for wearable and flexible energy storage devices such as supercapacitors. On the other hand, the drawback between high ionic conductivity and mechanical performance still becomes the typical problem to overcome. In this report, high stretchable and self-healing poly(lithium acrylate)-based hydrogels were prepared with free radical polymerization of lithium acrylate with vinyl silica nanoparticles as cross-linker and ammonium persulfate as initiator. Lithium acrylate was prepared by ion-exchanging acrylic acid with lithium hydroxide. Vinyl silica nanoparticles as cross-linkers acting as a stress buffer contribute to strengthening the polymer network under strain. Meanwhile, the self-healing ability is due to intramolecular and intermolecular hydrogen bonding of the hydrogel. Self-healing hydrogel polymer electrolytes were able to stretch up to 2000% and possessed high ionic conductivity ~10-3 S/cm at room temperature.
Lestari, Puji,Van, Kyujung,Kim, Moon Young,Lee, Byun-Woo,Lee, Suk-Ha National Research Council 2006 Canadian journal of microbiology Vol.52 No.4
<P> Supernodulating soybean (Glycine max L. Merr.) mutant SS2-2 and its wild-type counterpart, Sinpaldalkong 2, were examined for the microstructural events associated with nodule formation and development. SS2-2 produced a substantially higher percentage of curled root hairs than the wild type, especially at 14 days after inoculation with Bradyrhizobium japonicum. In addition, there was new evidence that in SS2-2, B. japonicum also entered through fissures created by the emerging adventitious root primordia. Early steps of nodule ontogeny were faster in SS2-2, and continued development of initiated nodules was more frequent and occurred at a higher frequency than in the wild type. These data suggest that the early expression of autoregulation is facilitated by decreasing the speed of cortical cell development, leading to the subsequent termination of less-developed nodules. The nodules of SS2-2 developed into spherical nodules like those formed on the wild type. In both the wild type and supernodulating mutant, vascular bundles bifurcate from root stele and branch off in the nodule cortex to surround the central infected zone. These findings indicate that SS2-2 has complete endosymbiosis and forms completely developed nodule vascular bundles like the wild type, but that the speed of nodule ontogeny differs between the wild type and SS2-2. Thus, SS2-2 has a novel symbiotic phenotype with regard to nodule organogenesis.Key words: Bradyrhizobium japonicum, early nodule development, Glycine max, root hair curling, supernodulation. </P>
Puji Lestari,최우혁 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Self-healing hydrogels were prepared through free radical polymerization of lithium acrylate monomer with the presence of vinyl silica nanoparticles as cross-linker and ammonium persulfate as initiator. The monomer was prepared by ion-exchanging acrylic acid with lithium hydroxide. Silica nanoparticles act as stress buffer to some degree strengthening the polymer network under strain, while hydrogen bonds contribute to self-healing properties of the hydrogel. Self-healing hydrogel polymer electrolytes were able to stretch up to 2000% and possessed high ionic conductivity of ~10<sup>-3</sup> S/cm at room temperature. Morphology, mechanical properties, self-healing properties, and electrochemical properties were systematically investigated with field emission–scanning electron microscopy (FE-SEM), universal testing machine (UTM), rheometer, and electrochemical impedance spectroscopy (EIS), respectively.
PCR Marker-Based Evaluation of the Eating Quality of <i>Japonica</i> Rice ( <i>Oryza sativa</i> L.)
Lestari, Puji,Ham, Tae-Ho,Lee, Ho-Hoon,Woo, Mi-Ok,Jiang, Wenzhu,Chu, Sang-Ho,Kwon, Soon-Wook,Ma, Kyungho,Lee, Jeong-Heui,Cho, Young-Chan,Koh, Hee-Jong American Chemical Society 2009 Journal of agricultural and food chemistry Vol.57 No.7
<P>Evaluation of eating quality in early breeding generations of rice is critical to developing varieties with better palatability. This paper reports DNA markers associated with eating quality of temperate <I>japonica</I> rice and an evaluation method aided by multiple regression analysis. A total of 30 markers comprising STSs, SNPs, and SSRs were tested for their association with palatability using 22 temperate <I>japonica</I> varieties with different palatability values. Eating quality-related traits of the 22 varieties were also measured. Of the 30 markers, 18 were found to be significantly associated with palatability and, consequently, a model regression equation with an <I>R</I><SUP>2</SUP> value of 0.99 was formulated to estimate the palatability by the marker data set. Validation of the model equation using selected breeding lines indicated that the marker set and the equation are highly applicable to evaluation of the palatability of cooked rice in temperate <I>japonica</I> varieties.</P>
Genetic diversity of mungbean (<i>Vigna radiata</i> L.) germplasm in Indonesia
Lestari, Puji,Kim, Sue Kyung,Reflinur,,Kang, Yang Jae,Dewi, Nurwita,Lee, Suk-Ha Cambridge University Press 2014 Plant genetic resources Vol.12 No.1
<P>Despite widespread mungbean [<I>Vigna radiata</I> (L.) Wilczek] consumption in Indonesia, few molecular studies have been carried out on accessions and available data are minimal. In this study, we used 30 newly developed simple sequence repeat (SSR) markers designed from the mapped sequence scaffolds of the Korean Sunhwanokdu and Gyeonggijaerae 5 mungbean genomes. These markers were used to examine loci in 83 mungbean accessions collected from diverse geographical areas in Indonesia. A total of 107 alleles were detected among the accessions with 29 polymorphic markers. However, the mean of polymorphic information content (0.33) value and diversity index (0.38) value was indicative of low genetic diversity in this germplasm. The mungbean population structure was not clearly differentiated and the number of subpopulations was unclear. Neighbour-joining tree analysis revealed that the genetic cluster did not reflect the geographical origin of the accessions. Interestingly, the most agriculturally improved varieties were genetically similar to some landraces from one of the main mungbean-producing regions. These newly developed SSR markers could be useful for detecting genetic variability as a basis for establishing a conservation strategy for mungbean germplasm with the aim of enhancing Indonesian breeding programmes.</P>
Single nucleotide polymorphism and haplotype diversity in the sucrose synthase 3 gene of rice
Puji Lestari,Gi An Lee,Tae-Ho Ham,Ho-Hoon Lee,Reflinur,Wenzhu Jiang,Mi-Ok Woo,Rihua Piao,Hee-Jong Koh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Sucrose synthase 3 which is a third active gene present in rice, is localized predominantly in rice endosperm. This sucrose synthase 3 may play an important role in the starch filling in the milky stage rice seed, probably involving in the starch physicochemical properties. As the genetic diversity at this locus is little informed, forty three rice consisting of japonica, indica and Oryza rufipogon were targeted to amplify full sequence of sucrose synthase 3 to examine the frequency and distribution of nucleotide polymorphism. Total of 755 all sequence variants detected, 491 single nucleotide polymorphisms (SNPs) and 264 indels were successfully identified in 7618 bp of sequence containing the sucrose synthase 3 transcript, promoter and 3' non-transcribed region. The frequency of nucleotide changes and indels were high, on average one polymorphism per 15.5 bp and one indel per 28.9 bp with 11 sequence-based haplotypes distinguishable among the varieties and lines. Both the frequency of nucleotide changes and indels were frequent in non-coding region, but rare in coding region. Sequencing a polymorphism region in the promoter showed one base change on one of cis-element from T (CATGCATA to A (CATGCACA) that might implicate in seed specificity. The presence of a high number of haplotype shared by a few varieties indicated a little information on linkage disequilibrium.
Single Nucleotide Polymorphism and Haplotype Diversity in the Rice Sucrose Synthase 3
Puji Lestari,Gi-An Lee,Tae-Ho Ham,Reflinur,Mi-Ok Woo,Rihua Piao,Wenzhu Jiang,Sang-Ho Chu,Joohyun Lee,Hee-Jong Koh 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
The rice sucrose synthase 3 (RSUS3) localized predominantly inrice seed endosperm may play an important role in the starch filling in the milky stage of rice seed. As the genetic diversity at this locus is not known yet, forty three rice varieties/accessions were objected to amplify full sequence of the RSUS3 to examine the distribution of DNA polymorphisms. A total of 254 sequence variants, including 82, 150 and 22SNP sand indels, were successfully identified in whole length of 7,733bp sequence comprising promoter, exon and intron, and 3’ down stream non transcribed region(NTR). Eleven haplotypes were distinguishable among 43 rice varieties based on the nucleotide variation on the three defined regions (5’NTR, transcript and 3’NTR). The promoter region showed the occurrence of a base change on a cis-element which might involve a functional role of the motif in seed-specific expression. Non random process seemed to be acted in the genetic diversity of RSUS3geneamongricegermplasmusedinthisstudy. The analysis of polymorphism sites indicated a history of eleven minimum recombination mostly occurred in the transcribed region. This result might provide an insight for a clasditic approach for establishing future genetic association studies of RSUS3locus.