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- 저자 : Puccinelli, J.P. (검색결과 3 건)
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Orlando Matteo,Trivellini Alice,Puccinelli Martina,Ferrante Antonio,Incrocci Luca,Mensuali-Sodi Anna 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.3
Crocus sativus L. is a crop grown for spice production, and large amounts of residues from the flowers are produced during the process. The underutilized by-product from saffron spice production, the C. sativus tepals, was investigated as a promising raw material of natural bioactive compounds using light spectrum manipulation in controlled environments. The plants were grown under either light-emitting diodes (LEDs) or natural light (NL, greenhouse). LED experiments were performed in controlled-environment chambers (120 µmol m–2 s–1of photosynthetically active radiation, 18 °C, 16-h photoperiod). The LED treatments used were as follows: (i) red ʎ = 660 nm (62%) and blue ʎ = 450 nm (38%) (RB); and (ii) red ʎ = 660 nm (50%), green ʎ = 500–600 nm (12%), and blue ʎ = 4 50 nm (38%) (RGB). Flower growth parameters, total phenols, total flavonoids, flavonols, flavonol glycosides, and antioxidant properties were measured in harvested tepals. Floral by-products from plants grown under the two LED treatments accumulated higher amounts of antioxidant compounds compared to those of plants grown under NL. The total flavonoids content was significantly enhanced in the RGB LED treatment, while the corolla fresh weight significantly declined in the same treatments. The higher content of bioactive secondary metabolites in plants grown under both RB and RGB light environments resulted in increased antioxidant capacity measured by DPPH free-radical scavenging capacity and the ferric reducing antioxidant power method. These results indicate that manipulation of LED spectra could boost secondary metabolites and antioxidant capacity to obtain phytochemically enriched floral by-products with superior functional quality.
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Automation of Three-Dimensional Cell Culture in Arrayed Microfluidic Devices
Montanez-Sauri, S.I.,Sung, K.E.,Puccinelli, J.P.,Pehlke, C.,Beebe, D.J. Association for Laboratory Automation 2011 Journal of laboratory automation Vol.16 No.3
The increasing interest in studying the interactions between cells and the extracellular matrix (ECM) has created a need for high throughput low-cost three-dimensional (3D) culture systems. The recent development of tubeless microfluidics via passive pumping provides a high throughput microchannel culture platform compatible with existing high throughput infrastructures (e.g., automated liquid handlers). Here, we build on a previously reported high throughput two-dimensional system to create a robust automated system for 3D culture. Operational controls including temperature and sample handling have been characterized and automated. Human mammary fibroblasts (HMFs) suspended in type I collagen are loaded and cultured in microchannel arrays and used to optimize the system operational parameters. A Peltier cooler maintains the collagen as a liquid at 4<SUP>o</SUP>C during cell seeding, followed by polymerization at 37<SUP>o</SUP>C. Optimization of this platform is discussed (e.g., controlling collagen contraction, increasing cell viability, preventing the removal of microchannel contents), and 3D distribution of HMFs is examined by fluorescent microscopy. Finally, we validate the platform by automating a previously developed 3D breast carcinoma coculture assay. The platform allows more efficient 3D culture experiments and lays the foundation for high throughput studies of cell-ECM interactions.
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