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      저자 : Prem R. Gupta (검색결과 2 건)
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        Modulation of Gamma Radiation-Inflicted Damage in Swiss Albino Mice by an Alcoholic Fraction of Podophyllum hexandrum Rhizome

        M.L. Gupta,P.K. Agrawala,Prem Kumar I,M. Devi,N.L. Soni,R.P. Tripathi 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.3

        A partially characterized extract of Podophyllum hexandrum rhizomes was studied for its radioprotective potential in mice. A major portion of the podophyllotoxin was obtained from the extract by further fractionation. Acute toxicity and maximum tolerated dose (MTD) of a single intraperitoneal dose of the extract were studied in mice to evaluate the toxicity of the extract, if any. Radioprotective efficacy was determined in terms of survival against 10 Gy whole-body irradiation (WBI), protection against 1 Gy-induced chromosomal aberration (CA), and estimation of dose reduction factor (DRF) in irradiated and extract pretreated mice. The MTD was observed to be 60 mg/kg of body weight, whereas a dose of 90 mg/kg of body weight yielded 50% death in mice within 72 hours of intraperitoneal administration of the extract. A dose range of 15–20 mg/kg of body weight administered 2 hours before 10 Gy WBI of mice yielded 66% survival, while administration of 10–15 mg/kg of body weight of the extract 1 hour before WBI yielded more than 90% survival. A DRF of 1.625 was estimated for 10 and 15 mg/kg of body weight of the extract administered 1 hour before WBI. Further studies on modulation of 1 Gy-induced CA revealed significant radioprotective efficacy of the extract in mouse bone marrow cells. Partial removal of podophyllotoxin was useful in reducing toxicity of the extract without altering its radioprotective efficacy.

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        Molecular evidence for the presence of the endosymbiontic bacteria Wolbachia in Cotesia populations (Hymenoptera: Braconidae)

        Rameshwar Singh Rattan,Ashok B. Hadapad,Annette Reineke,Prem R. Gupta,Claus P.W. Zebitz 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.2

        Cotesia are larval endoparasitoids and are potential biocontrol agents for various insect pests. In the present study, we attempted to detect the allele-specific ftsZ gene of Wolbachia in Cotesia spp. obtained from different geographical locations. Samples of Cotesia plutellae originating from India (Bangalore and Ludhiana), Kenya,and South Africa and samples of C. glomerata from India (Solan) confirmed the presence of Wolbachia. However, C. plutellae samples from the Kullu region of India did not show infection with Wolbachia. ftsZ sequences showed a high degree of homology within the species (N99%). The low genetic distance in the infected Cotesia populations indicated a single ancestral type, and distance to and from the outgroup suggested that populations are from the same species. Phylogenetic grouping of Cotesia species on the basis of geographic origin showed that the Wolbachia strain is closely related to strains that infect phylogenetically distant hosts.

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