Synthesis of Sr(1-x-y)Al4O7:Eux2+,Lny3+ (Ln = Dy, Y, Pr) Nanophosphors using Rapid Gel Combustion Process and their Down Conversion Characteristics

Devender Singh,Vijeta Tanwar,Anura Priyajith Samantilleke,Bernabe Mari,Shri Bhagwan,Krishan Chander Singh,Pratap Singh Kadyan,Ishwar Singh 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.3

Eu2+ and Eu2++Ln3+ doped SrAl4O7 nanophosphors were synthesized byrapid gel combustion process. The morphology of prepared phosphorswas examined with scanning and transmission electron microscopy. The phase identification and the crystal structures of nanophosphorswere studied using X-ray powder diffraction techniques. Luminescencecharacteristics of the prepared nanophosphors were analyzed on accountof excitation, emission and phosphorescence decay analysis. Theemission spectra demonstrated the broad green emission attributed to4f65d1→ 4f7 transition of the Eu2+ ions. The effect of codoping of sometrivalent lanthanide (Dy3+, Pr3+ and Y3+) ions were investigated forimproving the emission intensity and phosphorescence decay time ofthe basic lattice of SrAl4O7:Eu2+ phosphors. The synthesized materialshad enhanced bright luminescent properties that could suitably beapplied for display as well as photovoltaic applications.

Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2

Ajay Pratap Singh,Satparkash Singh,Santosh Kumar Gupta,Vijendra Pal Singh,Bhaskar Sharma,Rajeev Ranjan 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.3

Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of 1,011 bp, 1,008 bp and 1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A:3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (>90%) of homology. Furthermore, the plpE gene of Haemorhhagic septicaemia causing serotype (B: 2) was expressed in E. coli and recombinant PlpE was strongly immunostained by antiserum against whole cell antigen,indicating that the protein is expressed in vivo.

Evaluation of KiSS1 as a Prognostic Biomarker in North Indian Breast Cancer Cases

Singh, Richa,Bhatt, Madan Lal Brahma,Singh, Saurabh Pratap,Kumar, Vijay,Goel, Madhu Mati,Mishra, Durga Prasad,Kumar, Rajendra Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Breast cancer is the commonest female cancer worldwide and its propensity to metastasize negatively impacts on therapeutic outcome. Several clinicopathological parameters with prognostic/predictive significance have been associated with metastatic suppressor expression levels. The role of metastatic suppressor gene (MSG) KiSS1 in breast cancer remains unclear. Our goal was to investigate the possible clinical significance of KiSS1 breast cancer. Materials and Methods: The study was conducted on 87 histologically proven cases of breast cancer and background normal tiisue. Quantitative reverse transcriptase polymerase chain reaction (qRT PCR) and immunohistochemistry (IHC) were used to investigate KiSS1 at gene and protein levels, respectively, for correlation with several patient characteristics including age, family history, hormonal receptor status, stage, tumor size, nodal involvement and metastatic manifestation and finally with median overall survival (OS). Results: Our study revealed (i) KiSS1 levels were generally elevated in breast cancer vs normal tissue (P < 0.05). (ii) however, a statistically significant lower expression of KiSS1 was observed in metastatic vs non metastatic cases (P = 0.04). (iii) KiSS1 levels strongly correlated with T,N,M category, histological grade and advanced stage (p<0.001) but not other studied parameters. (iv) Lastly, a significant correlation between expression of KiSS1 and median OS was found (P = 0.04). Conclusions: Conclusively, less elevated KiSS1 expression is a negative prognostic factor for OS, advancing tumor stage, axillary lymph node status, metastatic propensity and advancing grade of the breast cancer patient. Patients with negative KiSS1 expression may require a more intensive therapeutic strategy.

Expression Levels of Tetraspanin KAI1/CD82 in Breast Cancers in North Indian Females

Singh, Richa,Bhatt, Madan Lal Brahma,Singh, Saurabh Pratap,Kumar, Vijay,Goel, Madhu Mati,Mishra, Durga Prasad,Srivastava, Kirti,Kumar, Rajendra Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7

Background: Carcinogenesis is a multifaceted intricate cellular mechanism of transformation of the normal functions of a cell into neoplastic alterations. Metastasis may result in failure of conventional treatment and death Hence, research on metastatic suppressors in cancer is a high priority. The metastatic suppressor gene CD82, also known as KAI1, is a member of the transmembrane 4 superfamily which was first identified in carcinoma of prostate. Little work has been done on this gene in breast cancer. Herein, we aimed to determine the gene and protein level expression of CD82/KAI1 in breast cancer and its role as a prognosticator. Materials and Methods: In this study, 83 histologically proven cases of breast cancer and a similar number of controls were included. Patient age ranged from 18-70 years. Quantitative Real Time Polymerase Chain Reaction (q-RT PCR) and immunohistochemistry (IHC) were used to investigate KAI1 expression at gene and protein levels, respectively. Statistical analysis was done to correlate expression of KAI1 and clinicopathological parameters. Results: It was revealed that: (i) KAI1 was remarkably diminished in metastatic vs non metastatic breast cancer both at the gene and the protein levels (P < .05); (ii) KAI1 expression levels were strongly correlated with TNM staging, histological grade and advanced stage (p<0.001) and no association was found with any other studied parameter; (iii) Lastly, a significant correlation was observed between expression of KAI1 and overall median survival of BC patients (P = 0.04). Conclusions: Our results suggest that lack of expression of the KAI1 might indicate a more aggressive form of breast cancer. Loss of KAI1 may be considered a significant prognostic marker in predicting metastatic manifestation. When evaluated along with the clinical and pathological factors, KAI1 expression may be beneficial to tailor aggressive therapeutic strategies for such patients.

Assessment of pregnancy-associated glycoprotein profile in milk for early pregnancy diagnosis in goats

Singh, Shiva Pratap,Natesan, Ramachandran,Sharma, Nandini,Goel, Anil Kumar,Singh, Manoj Kumar,Kharche, Suresh Dinkar Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.1

Objective: This study was conducted to assess the level of pregnancy-associated glycoprotein (PAG) in whole and skim milk samples, and its suitability for early pregnancy diagnosis in goats. Methods: A two-step sandwich enzyme-linked immunosorbent assay (ELISA) system for estimation of milk PAG was developed and validated, which employed caprine-PAG specific polyclonal antisera. Whole and skim milk samples (n = 210 each) from fifteen multiparous goats were collected on alternate days from d 10 to d 30, and thereafter weekly till d 51 post-mating. PAG levels in milk samples were estimated by ELISA and the pregnancies were confirmed at d40 post-mating by transrectal ultrasonography (TRUS). Results: The level of PAG in whole and skim milk samples of both pregnant and nonpregnant goats remained below the threshold values until d 24 after mating. Thereafter, PAG concentration in whole and skim milk increased steadily in pregnant goats, whereas it continued below the threshold in non-pregnant does. The PAG profiles in whole and skim milk of pregnant goats were almost similar and exhibited strong positive relationship (r = 0.891; p<0.001). Day 26 post-mating was identified as the first time-point for significantly (p<0.05) higher milk PAG concentration in pregnant goats than to non-pregnant goats. When compared to TRUS examination for pregnancy diagnosis, the accuracy and specificity of PAG ELISA using whole and skim milk samples were 94.5% and 95.4%; and 95.3% and 100%, respectively. The high values of area-under-curve (0.904 [whole milk] and 0.922 [skim milk]), demonstrate outstanding discrimination ability of the milk assays. Among the sampling dates chosen, d 37 post-mating was identified as the best suitable time point for collection of milk samples to detect pregnancy in goats. Conclusion: The PAG concentration in whole and skim milk of goats collected between days 26 and 51 post-breeding can be used for the accurate prediction of pregnancy and may be useful for assisting management decisions in goat flocks.

Spatially graded TiO???-SiO??? Bragg reflector with rainbow-colored photonic band gap.

Singh, Dhruv Pratap,Lee, Seung Hee,Choi, Il Yong,Kim, Jong Kyu Optical Society of America 2015 Optics express Vol.23 No.13

<P>A simple single-step method to fabricate spatially graded TiO2-SiO2 Bragg stack with rainbow colored photonic band gap is presented. The gradation in thickness of the Bragg stack was accomplished with a modified glancing angle deposition (GLAD) technique with dynamic shadow enabled by a block attached to one edge of the rotating substrate. A linear gradation in thickness over a distance of about 17 mm resulted in a brilliant colorful rainbow pattern. Interestingly, the photonic band gap position can be changed across the whole visible wavelength range by linearly translating the graded Bragg stack over a large area substrate. The spatially graded Bragg stack may find potential applications in the tunable optical devices, such as optical filters, reflection gratings, and lasers. (C) 2015 Optical Society of America</P>

Influence of intake runner cross section design on the engine performance parameters of a four stroke, naturally aspirated carbureted SI engine

Singh, Somendra Pratap,Kumar, Vasu,Gupta, Dhruv,Kumar, Naveen The International Promotion Agency of Culture Tech 2015 International Journal of Advanced Culture Technolo Vol.3 No.1

The current scenario of the transportation sector reflects the urgent need to address issues such as depletion of traditional fuel reserves and ever growing pollution levels. Researchers around the world are focussing on alternatives as well as optimisation of currently employed devices to reduce the pollution levels generated by the commonly used fuels. One such optimisation involves the study of air flow within the intake manifolds of SI engines. It is a well-known fact that alterations in the air manifolds of engines have a significant impact on the engine performance parameters, fuel consumption and emission levels. Previous works have demonstrated the impacts of runner lengths, diameter, plenum volume, taper angle of distribution manifolds and other factors on in-cylinder fluid motion and engine performance. However, a static setup provides an optimal configuration only at a specific engine speed. This paper aims to investigate the variations in the same parameters on a four stroke, naturally aspirated single cylinder SI engine through varying the cross section design over the intake runner with the aid of Computational Fluid Dynamics. The system consists of segments that form the intake runner with projections on the inside that allow various permutations of the intake runner segments. The various configurations provide the optimised fluid flow characteristics within the intake manifold at specific engine speed intervals. The variations such as turbulence, air fuel mixing are analysed using the three dimensional CFD software FLUENT. The results can be used further for developing an automated or manually adjustable intake manifold.

NEXAFS and XMCD studies of single-phase Co doped ZnO thin films

Singh, Abhinav Pratap,Kumar, Ravi,Thakur, P,Brookes, N B,Chae, K H,Choi, W K IOP Pub 2009 Journal of Physics, Condensed Matter Vol.21 No.18

<P>A study of the electronic structure and magnetic properties of Co doped ZnO thin films synthesized by ion implantation followed by swift heavy ion irradiation is presented using near-edge x-ray absorption fine structure (NEXAFS) and x-ray magnetic circular dichroism (XMCD) measurements. The spectral features of NEXAFS at the Co L<SUB>3,2</SUB>-edge show entirely different features than that of metallic Co clusters and other Co oxide phases. The atomic multiplet calculations are performed to determine the valence state, symmetry and the crystal field splitting, which show that in the present system Co is in the 2+ state and substituted at the Zn site in tetrahedral symmetry with 10<I>Dq</I> = −0.6 eV. The ferromagnetic character of these materials is confirmed through XMCD spectra. To rule out the possibilities of defect induced magnetism, the results are compared with Ar annealed and Ar-ion implanted pure ZnO thin films. The presented results confirm the substitution of Co at the Zn site in the ZnO matrix, which is responsible for room temperature ferromagnetism.</P>

Effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin

Singh, Payal,Nagpal, Rajni,Singh, Udai Pratap The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.3

Objectives: This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. Materials and Methods: Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups (n = 24) according to 5 different surface pre-treatments: No pre-treatment (control); 1M carbodiimide (EDC); 0.1% epigallocatechin-3-gallate (EGCG); 2% minocycline (MI); 10% sodium ascorbate (SA). After surface pre-treatment, adhesive (Adper Single Bond 2 [SB], 3M ESPE) was applied. Composite was applied into transparent plastic tubes (2.5 mm in diameter), which was placed over the bonded dentin surface. From each group, 10 samples were subjected to shear bond strength (SBS) evaluation at 24 hours (immediate) and remaining 10 samples were tested after 6 months (delayed). Additionally, 4 samples per group were subjected to scanning electron microscopic analysis for observation of resin-dentin interface. The data were statistically analysed with Shaperio-Wilk W test, 2-way analysis of variance (ANOVA), and post hoc Tukey's test. Results: At 24 hours, SBS of all surface pre-treatment groups were comparable with the control group, with significant differences found between EDC and SA groups only (p = 0.009). After 6 months storage, EDC, EGCG, and MI pre-treatments preserved the resindentin bond strength with no significant fall. Conclusions: Dentin pre-treatment with all the dentin biomodifiers except SA resulted in significant preservation of resin-dentin bond over 6 months storage period, without negatively affecting the immediate bond strength of the etch and rinse adhesive tested.

A Novel Technique for Contrast Target Detection in Through-the-Wall Radar Images

Singh Akhilendra Pratap,Dwivedi Smrity,Jain Pradip Kumar 한국전자파학회 2022 Journal of Electromagnetic Engineering and Science Vol.22 No.3

Through-the-wall radar images suffer from strong clutter due to the presence of dielectric walls and environmental noise. Several approaches have been explored to reduce clutter for detecting targets with similar dielectric constants. However, in real-time scenarios, targets may not have similar dielectric constants. Therefore, it is necessary to develop a technique for detecting contrast targets—that is, targets with different dielectric constants behind a wall in the same scene. This paper proposes a novel technique that combines time gating and Gaussian mixture model based low-rank approximation to detect contrast targets present in the same scene. The proposed method was compared to existing clutter reduction techniques in terms of target-to-clutter ratio (TCR). The experimental results showed that the proposed approach yielded highest TCR (130.1).