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Chiranjit Maity,Saptadip Samanta,Suman K. Halder,Pradeep K. Das Mohapatra,Bikas R. Pati,Malabendu Jana,Keshab C. Mondal 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2
The aim of this study was to produce two isozymes of α-amylase by immobilization of a newly isolated soil bacterium. The bacterium was identified as Bacillus thuringiensis CKB19 on the basis of its 16S rRNA profile. Enzyme production by free cells increased linearly with cell growth up to 34 h in starch containing enriched liquid media. The active bacterial cells were immobilized in Caalginate beads, and operational stability of the entrapped cell was optimized for amylase production. Enzyme production was optimal at an alginate concentration of 2 g%(w/v), calcium chloride concentration of 1 M, and with 300beads (each bead contained 2 × 10^7 cells)/250 mL flask. Amylase production by the immobilized cells was about 3times higher than free cell fermentation after 34 h of incubation. It was observed that the immobilized bacterium secreted two different amylases (Am-I and Am-II) into the culture fluid. The molecular masses of Am-I and Am-II were 59.6 and 44.7 kd, respectively, and showed optimum activity at pH 5.0 and 9.0. Both amylases showed optimum activity at 40℃ and were stable at the same temperature,with losses of only 10 and 20% (for Am I and Am II,respectively) of their original activities after 24 h of incubation. Further, both amylases were salt tolerant (up to 4 M NaCl) and hydrolyzed raw starchy foods into glucose. All these characteristics make this enzyme mixture suitable for use as a digestive aid and for the improvement of digestibility of animal feed ingredients.
안지현,김병주,김재근,김호진,홍계원,이희균,유재무,Ahn Ji-hyun,Kim Byeong-Joo,Kim Jae-Geun,Kim Ho-Jin,Hong Gye-Won,Lee Hee-Gyoun,Yoo Jai-Moo,Pradeep Halder 한국초전도학회 2005 Progress in superconductivity Vol.7 No.1
We investigated the surface oxidation behavior of cube-textured polycrystalline nickel at various oxidation conditions. Cube-textured NiO film was formed on a cube-textured polycrystalline nickel regardless of oxidation conditions but different growth behavior of NiO crystals was observed depending on the oxidation conditions. The introduction of water vapor into $O_2$ did not affect the texture evolution, but rough and porous microstructure was developed. Microstructure of NiO film tends to be denser as the oxygen partial pressure increases. It is interesting that (111) peak of theta - two theta diffraction pattern started to get stronger in air atmosphere and (111) plane became the major texture in the substrate oxidized in high purity argon gas. Small amount of high index crystallographic plane NiO peak crystal was observed when $N_{2}O$ was used as an oxidant while only (200) plane crystal was formed in dry $O_2$ atmosphere. Flat and smooth surface was changed into rough faceted one when ramping rate to oxidation temperature was faster. The grain size of NiO was decreased when the oxygen partial pressure was low. It was also observed that the modification of nickel surface suppressed the development of (200) texture.
MOD-TFA 공정에 의한 YBCO박막제조시 과잉 yttrium첨가 효과에 관한 연구
이승이,송슬아,김병주,박진아,김호진,이희균,홍계원,장석헌,주진호,유재무,Lee Seung-Yi,Song Seul-A,Kim Byeong-Joo,Park Jin-A,Kim Ho-Jin,Lee Hee-Gyoun,Hong Gye-Won,Jang Seog Heon,Joo Jinho,Yoo Jai-Moo,Pradeep Halder 한국초전도학회 2005 Progress in superconductivity Vol.7 No.1
[ $YBa_{2}Cu_{3}O_{7-x}$ ] thin films were fabricated on $LaAlO_3$(100) substrate by TFA-MOD process. Yttrium-excess (0, 2.5, 5, 10, 15, 20 $at\%$) coating solution was prepared by adding extra amount of yttrium into a stoichiometric(Y:Ba:Cu=1:2:3) TFA precursor solution. Results are presented concerning the influence of excess yttrium additions on the microstructure development and superconducting properties of $YBa_{2}Cu_{3}O_{7-x}$ film. Large sized CuO particles was observed by SEM EDS investigation. The addition of excess yttrium affected little on $T_c$ of $YBa_{2}Cu_{3}O_{7-x}$ film. $J_c$ of YBCO film was enhanced with excess yttrium addition. Jc maximum of $2.21\;MA/cm^2$ (77 K, self field) appeared with the $15\;at\%$ addition of excess yttrium. With further yttrium addition up to $20\;at\%$, Jc decreased down to $0.9\;MA/cm^2$.