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Piyush P. Sethia,K. Krishnamurthy Rao,Santosh B. Noronha 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
Escherichia coli is widely used for recombinantprotein production due to its well established geneticmanipulation techniques and cost effectiveness of theassociated production processes. Soluble expression ofheterologous recombinant proteins constitutes a majorproblem in the deployment of bacterial expression systems. We have developed a dps promoter based expressionsystem in E. coli for improved solubility of expressedproteins. The resulting expression system was found to besuperior to the IPTG inducible T7 promoter based pETexpression system for production of soluble β-galactosidase,tdTomato, and mCherry. The dps promoter based expressionsystem was shown to be functional in most commonlyused strains of E. coli without need for prior geneticmanipulation of the host genome.
Flame retardancy of nanocomposites with emphasis on Halloysite nanotubes
Deepak Rawtani,Swath Satish,Piyush Rao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-
Industrial and academic communities have been paying close attention to developments in polymernanocomposite technology. Nanomaterials can be combined to create nanocomposites with goal ofenhancing specific physical, thermal, and other characteristics. Materials such as choice of clay, pretreatment,choice of polymers and their component, and method of incorporating polymers within thenanocomposites, all have a bearing on the nature and final qualities of resulting nanocomposites. Theproperties of composites are considerably changed when nanoparticles are added to polymers, but theeffects rely greatly on how the nanomaterials are processed and surface-treated. To create a nanocompositewith the requisite physical and mechanical qualities, a nanomaterial is mixed with a polymer. In comparison to other clays, surface features of Halloysite nanotubes (HNTs) provide opportunities forsurface modifications. Besides, due to inter-tubular and interfacial interactions between HNT and polymeras well as structure of HNT, the thermal properties of nanocomposites are enhanced. In this review,various properties of HNT necessary for fire retardancy of nanocomposites are discussed. Over the pastdecade, Halloysite has been recognized as nanomaterial of choice with different polymers to act as synergistfor thermal stability and regulate the flame retardancy behaviour of resultant nanocomposite.