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        Wireless sensor networks for permanent health monitoring of historic buildings

        Zonta, Daniele,Wu, Huayong,Pozzi, Matteo,Zanon, Paolo,Ceriotti, Matteo,Mottola, Luca,Picco, Gian Pietro,Murphy, Amy L.,Guna, Stefan,Corra, Michele Techno-Press 2010 Smart Structures and Systems, An International Jou Vol.6 No.5

        This paper describes the application of a wireless sensor network to a 31 meter-tall medieval tower located in the city of Trento, Italy. The effort is motivated by preservation of the integrity of a set of frescoes decorating the room on the second floor, representing one of most important International Gothic artworks in Europe. The specific application demanded development of customized hardware and software. The wireless module selected as the core platform allows reliable wireless communication at low cost with a long service life. Sensors include accelerometers, deformation gauges, and thermometers. A multi-hop data collection protocol was applied in the software to improve the system's flexibility and scalability. The system has been operating since September 2008, and in recent months the data loss ratio was estimated as less than 0.01%. The data acquired so far are in agreement with the prediction resulting a priori from the 3-dimensional FEM. Based on these data a Bayesian updating procedure is employed to real-time estimate the probability of abnormal condition states. This first period of operation demonstrated the stability and reliability of the system, and its ability to recognize any possible occurrence of abnormal conditions that could jeopardize the integrity of the frescos.

      • The Arabidopsis vacuolar malate channel is a member of the ALMT family

        Kovermann, Peter,Meyer, Stefan,,rtensteiner, Stefan,Picco, Cristiana,Scholz-Starke, Joachim,Ravera, Silvia,Lee, Youngsook,Martinoia, Enrico Blackwell Publishing Ltd 2007 The Plant journal Vol.52 No.6

        <P>Summary</P><P>In plants, malate is a central metabolite and fulfills a large number of functions. Vacuolar malate may reach very high concentrations and fluctuate rapidly, whereas cytosolic malate is kept at a constant level allowing optimal metabolism. Recently, a vacuolar malate transporter (<I>Arabidopsis thaliana</I> tonoplast dicarboxylate transporter, <I>At</I>tDT) was identified that did not correspond to the well-characterized vacuolar malate channel. We therefore hypothesized that a member of the aluminum-activated malate transporter (ALMT) gene family could code for a vacuolar malate channel. Using GFP fusion constructs, we could show that <I>At</I>ALMT9 (<I>A. thaliana</I> ALMT9) is targeted to the vacuole. Promoter-GUS fusion constructs demonstrated that this gene is expressed in all organs, but is cell-type specific as GUS activity in leaves was detected nearly exclusively in mesophyll cells. Patch-clamp analysis of an <I>Atalmt9</I> T-DNA insertion mutant exhibited strongly reduced vacuolar malate channel activity. In order to functionally characterize <I>At</I>ALMT9 as a malate channel, we heterologously expressed this gene in tobacco and in oocytes. Overexpression of <I>At</I>ALMT9-GFP in <I>Nicotiana benthamiana</I> leaves strongly enhanced the malate current densities across the mesophyll tonoplasts. Functional expression of <I>At</I>ALMT9 in <I>Xenopus</I> oocytes induced anion currents, which were clearly distinguishable from endogenous oocyte currents. Our results demonstrate that <I>At</I>ALMT9 is a vacuolar malate channel. Deletion mutants for <I>At</I>ALMT9 exhibit only slightly reduced malate content in mesophyll protoplasts and no visible phenotype, indicating that <I>At</I>tDT and the residual malate channel activity are sufficient to sustain the transport activity necessary to regulate the cytosolic malate homeostasis.</P>

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        Wireless sensor networks for permanent health monitoring of historic buildings

        Daniele Zonta,Huayong Wu,Matteo Pozzi,Paolo Zanon,Matteo Ceriotti,Luca Mottola,Gian Pietro Picco,Amy L. Murphy,Stefan Guna,Michele Corrà 국제구조공학회 2010 Smart Structures and Systems, An International Jou Vol.6 No.6

        This paper describes the application of a wireless sensor network to a 31 meter-tall medieval tower located in the city of Trento, Italy. The effort is motivated by preservation of the integrity of a set of frescoes decorating the room on the second floor, representing one of most important International Gothic artworks in Europe. The specific application demanded development of customized hardware and software. The wireless module selected as the core platform allows reliable wireless communication at low cost with a long service life. Sensors include accelerometers, deformation gauges, and thermometers. A multi-hop data collection protocol was applied in the software to improve the system flexibility and scalability. The system has been operating since September 2008, and in recent months the data loss ratio was estimated as less than 0.01%. The data acquired so far are in agreement with the prediction resulting a priori from the 3-dimensional FEM. Based on these data a Bayesian updating procedure is employed to real-time estimate the probability of abnormal condition states. This first period of operation demonstrated the stability and reliability of the system, and its ability to recognize any possible occurrence of abnormal conditions that could jeopardize the integrity of the frescos.

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