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Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam
Kim Cuc Thi Nguyen,Phuc Hung Truong,Cuong Tu Ho,Cong Tuan Le,Khoa Dang Tran,Tien Long Nguyen,Manh Tuan Nguyen,Phu Van Nguyen 한국균학회 2023 Mycobiology Vol.51 No.6
In this study, twenty-five yeast strains were isolated from soil samples collected in the goldmining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerateCu2þ up to 10 mM, and the isolates could also grow in a wide range of pH (3–7), and temperature(10–40 �C). Dried biomass of GL1 was able to remove Cu2þ effectively up to 90.49%with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 �C, and incubationtime 60 min. Sequence analysis of rDNA indicated this strain was closely related toRhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strainappears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts andit may produce a new tool for environmental control and metal recovery operations.
Nguyen Kim Cuc Thi,Phuc Hung Truong,Hoa Truong Thi,Xuan Tuy Ho,Phu Van Nguyen 질병관리청 2024 Osong Public Health and Research Persptectives Vol.15 No.1
Objectives: Vibrio parahaemolyticus is a major foodborne pathogen in aquatic animals and a threat to human health worldwide. This study investigated the prevalence, antimicrobial resistance, antimicrobial resistance genes (ARGs), and biofilm formation of V. parahaemolyticus strains isolated from fish mariculture environments in Cat Ba Island, Vietnam.Methods: In total, 150 rearing water samples were collected from 10 fish mariculture farms in winter and summer. A polymerase chain reaction assay was used to identify V. parahaemolyticus, its virulence factors, and ARGs. The antimicrobial resistance patterns and biofilm formation ability of V. parahaemolyticus strains were investigated using the disk diffusion test and a microtiter plate-based crystal violet method, respectively.Results: Thirty-seven V. parahaemolyticus isolates were recovered from 150 samples. The frequencies of the tdh and trh genes among V. parahaemolyticus isolates were 8.1% and 21.6%, respectively. More than 90% of isolates were susceptible to ceftazidime, cefotaxime, and chloramphenicol, but over 72% were resistant to ampicillin, tetracycline, and erythromycin. Furthermore, 67.57% of isolates exhibited multidrug resistance. The presence of ARGs related to gentamicin (aac(3)-IV), tetracycline (tetA) and ciprofloxacin (qnrA) in V. parahaemolyticus isolates was identified. Conversely, no ARGs related to ampicillin or erythromycin resistance were detected. Biofilm formation capacity was detected in significantly more multidrug-resistant isolates (64.9%) than non-multidrug-resistant isolates (18.9%).Conclusion: Mariculture environments are a potential source of antibiotic-resistant V. parahaemolyticus and a hotspot for virulence genes and ARGs diffusing to aquatic environments. Thus, the prevention of antibiotic-resistant foodborne vibriosis in aquatic animals and humans requires continuous monitoring.