하반신마비 환자들을 위한 손자전거

Stefan Pfeifer,Dominik Peter Scholl 현대건축사 2001 월간 CONCEPT Vol.- No.33

〈긴급사태에 응답하여〉위스콘신 점령 운동이 퍼져나가다

브라이언 파이퍼(Bryan G. Pfeifer),위스콘신 밀워키(Milwaukee, Wis),장진엽(번역자) 노동사회과학연구소 2012 정세와노동 Vol.- No.76

Accurate Measurement of the Relative Abundance of Different DNA Species in Complex DNA Mixtures

Jeong, Sangkyun,Yu, Hyunjoo,Pfeifer, Karl Oxford University Press 2012 DNA research Vol.19 No.3

<P>A molecular tool that can compare the abundances of different DNA sequences is necessary for comparing intergenic or interspecific gene expression. We devised and verified such a tool using a quantitative competitive polymerase chain reaction approach. For this approach, we adapted a competitor array, an artificially made plasmid DNA in which all the competitor templates for the target DNAs are arranged with a defined ratio, and melting analysis for allele quantitation for accurate quantitation of the fractional ratios of competitively amplified DNAs. Assays on two sets of DNA mixtures with explicitly known compositional structures of the test sequences were performed. The resultant average relative errors of 0.059 and 0.021 emphasize the highly accurate nature of this method. Furthermore, the method's capability of obtaining biological data is demonstrated by the fact that it can illustrate the tissue-specific quantitative expression signatures of the three housekeeping genes <I>G6pdx</I>, <I>Ubc</I>, and <I>Rps27</I> by using the forms of the relative abundances of their transcripts, and the differential preferences of <I>Igf2</I> enhancers for each of the multiple <I>Igf2</I> promoters for the transcription.</P>

Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells

김재영,윤화영,Karl Pfeifer,은복기 한국통합생물학회 2019 Animal cells and systems Vol.23 No.4

Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements – enhancers and insulators – in organizing chromatin and gene expression across a large domain. In this study we characterize regulated expression of the Igf2 antisense transcript (Igf2as) in primary muscle cells. We demonstrate that Igf2as is imprinted (expressed only from the paternal chromosome). We also show that Igf2as expression during differentiation follows the same patterns as Igf2 and H19. Moreover, this expression is dependent upon the same shared enhancer element. Thus, our work shows that the imprinted cluster includes Igf2as in addition to H19, Igf2, and Nctc1.

THE NEW DEUTZ ENGINE FAMILY 2013 FOR COMMERCIAL VEHICLES

Mikulic, L.,Pleimling, H.,Pfeifer, A.,Lingens, A. The Korean Society of Automotive Engineers 2001 International journal of automotive technology Vol.2 No.4

DEUTZ AG, co-founded in 1864 by Nicolaus August Otto, the inventor of the four-stroke cycle engine, has developed the new 2013 engine for commercial vehicles on the basis of the tried and tested 1012 and 1013 series. With 4 and 6 cylinder models, the engine covers the power range between 100 and 190 kW. At the time of their introduction to the market, the engines will meet the exhaust emission legislation of EURO IV and incorporate the potential for EURO IV, Further engineering targets were. (Compactness, Favourable power/cost relation, Low weight, Low fuel consumption and Low noise level). All targets could be accomplished in a relatively short development period via the application of modem simulation tools and test methods. In this paper, the design configuration of the engines is described with particular emphasis on measures for noise emission reduction and the combustion system including injection and turbo charging. Furthermore, we demonstrate the engine's potential to fullfill the European emission legislation EU4, which comes into force in 2005.

THE NEW DEUTZ ENGINE FAMILY 2013 FOR COMMERCIAL VEHICLES

L. MIKULIC. H. PLEIMLING,A.PFEIFER,A. LINGENS 한국자동차공학회 2001 International journal of automotive technology Vol.2 No.4

DEUTZ AG, co-founded in 1864 by Nicolaus August Otto. the inventor of the four-stroke cycle engine, has developed the new 2013 engine for commercial vehicles on the basis of the tried and tested 1012 and 1013 series. With 4 and 6 cylinder models, the engine covers the power range between 100 and 190 kW. At the time of their introduction to the market. the engines will meet the exhaust emission legislation of EURO III and incorporate the potential for EURO IV. Further engineering targets were: (Compactness. Favourable power/cost relation, Low weight, Low fuel consumption and Low noise level). All targets could be accomplished in a relatively short development period via the application of modern simulation tools and test methods. In this paper, the design configuration of the engines is described with particular emphasis on measures for noise emission reduction and the combustion system including injection and turbo charging. Furthermore. we demonstrate the engine's potential to fullfill the European emission legislation EU4. which comes into force in 2005.<br/> <br/>

The therapeutic efficacy of Qigong exercise on the main symptoms of fibromyalgia: a pilot randomized clinical trial

Caio V.M. Sarmento,Sanghee Moon,Taylor Pfeifer,Irina V. Smirnova,Yvonne Colgrove,Sue Min Lai,Wen Liu 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.4

Background: Some of the most debilitating symptoms of fibromyalgia (FM) include widespread chronic pain, sleep disturbances, chronic fatigue, anxiety, and depression. Yet, there is a lack of effective self-management exercise interventions capable of alleviating FM symptoms. The objective of this study is to examine the efficacy of a 10-week daily Qigong, a mind–body intervention program, on FM symptoms. Methods: 20 participants with FM were randomly assigned to Qigong (experimental) or sham-Qigong (control) groups, with participants blinded to the intervention allocation. The Qigong group practiced mild body movements synchronized with deep diaphragmatic breathing and meditation. The sham-Qigong group practiced only mild body movements. Both groups practiced the interventions two times per day at home, plus one weekly group practice session with a Qigong instructor. Primary outcomes were: pain changes measured by the Short-Form McGill Pain Questionnaire, a visual analog scale for pain, pressure pain threshold measured by a dolorimeter. Secondary outcomes were: the Revised Fibromyalgia Impact Questionnaire the Pittsburgh Sleep Quality Index, the Hospital Anxiety and Depression Scale and the Quality of Life Scale. Results: The experimental group experienced greater clinical improvements when compared to the control group on the mean score differences of pain, sleep quality, chronic fatigue, anxiety, depression, and fibromyalgia impact, all being statistically significant at p < 0.05. Conclusion: Daily practice of Qigong appears to have a positive impact on the main fibromyalgia symptoms that is beyond group interaction. Trial registration: ClinicalTrials.gov NCT03441997.

Mutation Spectra Induced by Adozelesin in the supF Gene of Human XP-A Fibroblasts

Se-Young Lee,Gerd P. Pfeifer,이종순 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.4

Adozelesin is a synthetic analog of the antitumor antibiotic, CC-1065, which alkylates N3 of adenine in the minor DNA groove in a sequence-specific manner. Here we tested the mutation spectra induced by adozelesin in the supF gene of human XP-A fibroblasts using a shuttle vector assay. Adozelesin primarily induces mutations via an A → T transversion and a single base insertion. The A → T transversion (43/59) was observed at the adenine alkylation site in the 5’-ATTTA* sequence (A* is the site of alkylation). The single base insertion (5/59) was observed at the 3’-side of the covalently modified adenine in the 5’-CTAAA* sequence. The results of this study suggest that the DNA alkylating sequence of adozelesin influences the type of DNA mutation.

Skin Testing With Peach Peel Extract Versus Serum IgE to Pru p 3 as a Stronger Predictor of Peach-Induced Anaphylaxis

Somoza Maria Luisa,Prieto-Moreno Pfeifer Ana,Martín-Pedraza Laura,Victorio Puche Laura,Esteban Rodríguez Angel,Blanca-López Natalia,Eva Fernández González Abel,Fernández-Caldas Enrique,Morán Morales M 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.6

The most important peach fruit allergen is Pru p 3, followed by Pru p 1, Pru p 4, and Pru p 7. We aimed to assess their role in subjects with peach fruit-induced allergy (anaphylaxis and OAS) and compare skin prick tests (SPT) vs. specific immunoglobulin E (sIgE) for predicting anaphylaxis. We also selected a control group. SPT included prevalent inhalant and plant food allergens plus peach peel extract. The sIgE to Pru p 1, Pru p 3, Pru p 4, and Pru p 7 were quantified. Compared with controls (n = 42), cases (n = 41) were younger (P = 0.003), more frequently female (P < 0.05) and had higher SPT positivity to peach peel (44% vs. 2.4%, P < 0.0001). There were significant differences in sensitization to several pollens: Olea europaea, Artemisia vulgaris, Prunus persica, Platanus acerifolia (all P < 0.001); and fruits: apple (P < 0.04), peanut (P < 0.002), tomato (P < 0.005), and melon (P < 0.05). Pru p 3 sIgE was detected in 61% of all cases (85% anaphylaxis and 38% OAS; P < 0.01 each) and 5% of controls (P < 0.001). Pru p 4 sIgE was present in 19% of cases and 7% of controls. The sIgE to Pru p 1 and Pru p 7 were not found. The odds ratio to predict anaphylaxis for peach peel SPT was 113 (confidence interval [CI], 20–613; P < 0.0001); for sIgE to Pru p 3, 22 (CI, 5.3–93; P < 0.0001); and for SPT positivity to selected plant food allergens, 5 (CI, 1–19; P < 0.05). In our study group, SPT with peel peach extract was a better predictor of anaphylaxis than Pru p 3 sIgE or other variables considered. The role of sIgE to Pru p 1, Pru p 4, and Pru p 7 seemed negligible.

Mutation spectra induced by 1-nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide in the supF gene of human XP-A fibroblasts

Kim, Byung-Wook,Kim, Byung-Chun,Cha, Jin-Soon,Pfeifer, Gerd P.,Lee, Chong-Soon Korean Society for Biochemistry and Molecular Biol 2008 BMB Reports Vol.41 No.8

1-Nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide are oxidative metabolites that are responsible for the mutagenicity of 1-nitropyrene. In this study, the mutation spectra induced by oxidative metabolites in human cells were determined using a shuttle vector assay. The mutation frequencies induced by 1-nitropyrene 9,10-oxide were 2-3 times higher than those induced by 1-nitropyrene 4,5-oxide. The base substitutions induced by 1-nitropyrene 4,5-oxide were $G{\rightarrow}A$ transitions, $G{\rightarrow}C$ transversions, and $G{\rightarrow}T$ transversions. In the case of 1-nitropyrene 9,10-oxide, $G{\rightarrow}A$ transitions, $G{\rightarrow}T$ transversions, $A{\rightarrow}G$ transitions and $G{\rightarrow}C$ transversions were observed. Most base substitution mutations induced by oxidative metabolites occurred at the guanine sites in the supF gene. These sequence-specific hot spots were commonly identified as 5'-GA sequences for both metabolites. On the other hand, the sequence-specific hot spots at the adenine sites were identified as 5'-CAC sequences for 1-nitropyrene 9,10-oxide. These results suggest that the oxidative metabolites of 1-nitropyrene induce sequence-specific DNA mutations at the guanine and adenine sites at high frequency.