The human AQP4 gene : Definition of the locus encoding two water channel polypeptides in brain

LU, MINGOI,LEE, M.DOUGLAS,SMITH, BARBARA L.,JUNG, JIN SUP,AGRE, PETER,VERDUK, MARIAN A.J.,MERKX, GERARD,RUSS, JOHAN P.L.,DEEN, PETER M.T. 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

The aquaporin family of membrane water transport proteins are expressed in diverse tissues, and in brain the predominant water channel protein is AQP4. Here we report the isolation and characterization of the human AQP4 cDNAs and genomic DNA. Two cDNAs were isolated corresponding to the two initiating methionines (M1 in a 323-aa polypeptide and M23 in a 301-aa polypeptide) previously indentified in rat [Jung, J. S., Bhat, R. V., Preston, G. M., Guggino, W. B. & Agre, P. (1994) Proc. Natl. Acad. Sci. USA 91, 13052-13056]. Similar to other aquaporins, the AQP4 gene is composed of four exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. Transcription initiation sites have been mapped in the proximal promoters of exons 0 and 1. RNase protection revealed distinct transcripts corresponding to M1 and M23 mRNAs, and AQP4 immunoblots of cerebellum demonstrated reactive polypeptides of 31 and 34 kDa. Using a P1 and a λEMBL subclone, the chromosomal site of the human AQP4 gene was mapped to chromosome 18 at the junction of q11.2 and q12.1 by fluorescence in situ hybridization. These studies may now permit molecular characterization of AQP4 during human development and in clinical disorders.

THE EFFECT OF HOST GALAXIES ON TYPE Ia SUPERNOVAE IN THE SDSS-II SUPERNOVA SURVEY

Lampeitl, Hubert,Smith, Mathew,Nichol, Robert C.,Bassett, Bruce,Cinabro, David,Dilday, Benjamin,Foley, Ryan J.,Frieman, Joshua A.,Garnavich, Peter M.,Goobar, Ariel,Im, Myungshin,Jha, Saurabh W.,Marrin IOP Publishing 2010 The Astrophysical journal Vol.722 No.1

The Interplay Between Supervisor Safety Support and Occupational Health and Safety Vulnerability on Work Injury

Yanar, Basak,Lay, Morgan,Smith, Peter M. Occupational Safety and Health Research Institute 2019 Safety and health at work Vol.10 No.2

Background: Workers exposed to hazards without adequate protections are at greater risk of injury and illness. Supervisor activities have also been associated with injury risk. We examined the interplay between supervisor safety support and occupational health and safety (OHS) vulnerability on workplace injury and illness. Methods: A survey was administered to 2,390 workers employed for more than 15 hrs/week in workplaces with at least five employees who had a direct supervisor. We examined the combined effects of hazard exposure with inadequate protection (OHS vulnerability) and supervisor support on workplace injury and illness, using additive interactions in log-binomial regression models. Results: OHS vulnerability and lack of supervisor support independently increased the likelihood of physical injuries at work. Crude and adjusted models showed that the risk of physical injury was at least 3.5 times higher among those experiencing both OHS vulnerability and a lack of supervisor support than individuals without OHS vulnerability and with a supportive direct supervisor. Workers who experienced vulnerability were at less risk if they had a supervisor who was supportive. Conclusion: In workplaces where workers experience one or more types of OHS vulnerability, having a supportive supervisor may play an important role in reducing the risk of injury and protecting workers.

The Impact of Adverse Employment and Working Conditions on the Risk of Workplace Injury in Canada

Nadalin Victoria,Mustard Cameron,Smith Peter M. 한국산업안전보건공단 산업안전보건연구원 2021 Safety and health at work Vol.12 No.4

Background: Employment standards (ES) include having a regular payday, regular breaks, the right to paid sick or vacation time, and paid wages. Inadequate ES contribute to the labour market vulnerability of workers; however, they are not typically considered to be risk factors for workplace injury. In a sample of Canadian workers, we examine the risk of injury associated with inadequate ES, independent of, and combined with inadequate workplace protections from workplace hazards. Methods: Data from 2,803 adults working 15 hours or more/week in workplaces with at least five employees were analysed. We explored associations between exposure to workplace hazards with inadequate protections [termed occupational health and safety (OHS) vulnerability] and inadequate ES on workplace injury (physical or mental injury; injury requiring time off). Additive interaction models were used to examine the independent and combined effects of these exposures. Results: Occupational health and safety vulnerability and inadequate ES were independently associated with increased injury outcomes. Adjusted models showed an additive relationship for all injury outcomes between OHS vulnerability and inadequate ES. Statistically significant superadditive relationships were observed for physical injury risk with policy and procedure vulnerability plus inadequate ES [synergy index (S) 1.50, 95% CI: 1.13-2.00] and for overall OHS vulnerability plus inadequate ES (S 1.53, 95% CI: 1.16-2.02), suggesting a combined effect greater than independent effects. Conclusion: Occupational health and safety vulnerability and inadequate ES are independently associated with workplace injury. For certain injury outcomes, the combined effect of OHS vulnerability and inadequate ES is greater than the independent effects of each individual exposure.

The Interplay Between Supervisor Safety Support and Occupational Health and Safety Vulnerability on Work Injury

Basak Yanar,Morgan Lay,Peter M. Smith 한국산업안전보건공단 산업안전보건연구원 2019 Safety and health at work Vol.10 No.2

Background: Workers exposed to hazards without adequate protections are at greater risk of injury and illness. Supervisor activities have also been associated with injury risk. We examined the interplay between supervisor safety support and occupational health and safety (OHS) vulnerability on workplace injury and illness. Methods: A survey was administered to 2,390 workers employed for more than 15 hrs/week in workplaces with at least five employees who had a direct supervisor. We examined the combined effects of hazard exposure with inadequate protection (OHS vulnerability) and supervisor support on workplace injury and illness, using additive interactions in log-binomial regression models. Results: OHS vulnerability and lack of supervisor support independently increased the likelihood of physical injuries at work. Crude and adjusted models showed that the risk of physical injury was at least 3.5 times higher among those experiencing both OHS vulnerability and a lack of supervisor support than individuals without OHS vulnerability and with a supportive direct supervisor.Workers who experienced vulnerability were at less risk if they had a supervisor who was supportive. Conclusion: In workplaces where workers experience one or more types of OHS vulnerability, having a supportive supervisor may play an important role in reducing the risk of injury and protecting workers.

Acoustic phonon lifetimes limit thermal transport in methylammonium lead iodide

Gold-Parker, Aryeh,Gehring, Peter M.,Skelton, Jonathan M.,Smith, Ian C.,Parshall, Dan,Frost, Jarvist M.,Karunadasa, Hemamala I.,Walsh, Aron,Toney, Michael F. National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.47

<▼1><P><B>Significance</B></P><P>Hybrid organic–inorganic perovskites are a promising class of materials for efficient and low-cost solar cells. Unlike conventional inorganic semiconductors such as silicon and gallium arsenide, hybrid perovskites feature significant dynamic disorder in their crystal structure. This dynamic disorder can be broadly classified into lattice vibrations (phonons) and molecular rotations. Phonons interact with charge carriers through electron–phonon coupling, which has substantial impacts on the operation of solar cells. Our study shows that acoustic phonons, the type responsible for transmitting heat in conventional semiconductors, have extraordinarily short lifetimes in the archetypal hybrid perovskite methylammonium lead iodide. These short lifetimes have direct implications on the cooling and transport of electrons and reflect a key difference between hybrid perovskites and conventional photovoltaic semiconductors.</P></▼1><▼2><P>Hybrid organic–inorganic perovskites (HOIPs) have become an important class of semiconductors for solar cells and other optoelectronic applications. Electron–phonon coupling plays a critical role in all optoelectronic devices, and although the lattice dynamics and phonon frequencies of HOIPs have been well studied, little attention has been given to phonon lifetimes. We report high-precision momentum-resolved measurements of acoustic phonon lifetimes in the hybrid perovskite methylammonium lead iodide (MAPI), using inelastic neutron spectroscopy to provide high-energy resolution and fully deuterated single crystals to reduce incoherent scattering from hydrogen. Our measurements reveal extremely short lifetimes on the order of picoseconds, corresponding to nanometer mean free paths and demonstrating that acoustic phonons are unable to dissipate heat efficiently. Lattice-dynamics calculations using ab initio third-order perturbation theory indicate that the short lifetimes stem from strong three-phonon interactions and a high density of low-energy optical phonon modes related to the degrees of freedom of the organic cation. Such short lifetimes have significant implications for electron–phonon coupling in MAPI and other HOIPs, with direct impacts on optoelectronic devices both in the cooling of hot carriers and in the transport and recombination of band edge carriers. These findings illustrate a fundamental difference between HOIPs and conventional photovoltaic semiconductors and demonstrate the importance of understanding lattice dynamics in the effort to develop metal halide perovskite optoelectronic devices.</P></▼2>

Measuring low-energy ( α , p ) reaction cross sections using an extended gas target and gas recirculator

Chae, K.Y.,Ahn, S.,Ayres, A.,Bardayan, D.W.,Bey, A.,Greife, U.,Howard, M.E.,Jones, K.L.,Kozub, R.L.,Matoš,, M.,Moazen, B.H.,Nesaraja, C.D.,O’Malley, P.D.,Peters, W.A.,Pittman, S.T.,Smith, M.S. Elsevier 2018 Nuclear Instruments & Methods in Physics Research. Vol.900 No.-

<P><B>Abstract</B></P> <P>Direct measurements of ( α , p ) reactions of astrophysical interest with radioactive beams presents serious challenges because of the difficult nature of helium targets and the typical low intensities of the beams. To address this, a new technique has been developed for measurements of low-energy ( α , p ) reactions with heavy ion beams using an extended <SUP> 4 </SUP> He gas target and a newly developed gas recirculating system. The system was used to measure the <SUP> 4 </SUP> He(<SUP>19</SUP>F, <SUP> 1 </SUP> H)<SUP>22</SUP>Ne reaction as a demonstration. Excitation functions of the <SUP>19</SUP>F( α , p )<SUP>22</SUP>Ne and <SUP>19</SUP>F( α , <SUP> p ′ </SUP> )<SUP>22</SUP>Ne <SUP> ∗ </SUP> reactions were successfully measured to show the viability of this technique. Details of the approach and future plans are given.</P>