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- 저자 : Paula S. Lunardi (검색결과 2 건)
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Thaís Peres Câmara,Letícia Pinho Gomes,Rosaline Rocha Lunardi,Paula Cristina de Souza Souto,Adriana Degrossoli,Wagner Welber Arrais-Silva 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
The bloodsucking bug Triatoma williami is the main vector of Trypanosoma cruzi, the etiological agent of Chagas disease in State of Mato Grosso, Brazil. It occurs in sylvatic areas of Brazilian savanna with often records of adult specimens in domestic and peridomestic habitats. We describe, for the first time, the reproductive parameters of T. williami females such as fecundity, fertility and lifespan. We also evaluate whether mating and nourishment status of females of T. williami may modulate the reproductive parameters. As a result, the presence of male corroborated to increase female lifespan from 5.14 ± 1,77 to 34.57 ± 10,86 weeks. The oviposition rate in mated female increased 275% when we compared to the fecundity values in unmated females. Finally, our results indicate that mating is crucial to improve lifespan and fecundity of T. williami females. This characteristic may be related to the environmental conditions under T. williami develops, because the sylvatic ecotopes form small scattered colonies with infrequent access to blood sources.
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Genoprotective Effects of the Green Tea-Derived Polyphenol/Epicatechin Gallate in C6 Astroglial
Renata T. Abib,Andre´ Quincozes-Santos,Caroline Zanotto,Fares Zeida´n-Chulia,Paula S. Lunardi,Carlos-Alberto Gonc¸alves,Carmem Gottfried 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
In vitro and in vivo studies have recently reported significant chemopreventive effects of green tea-derived polyphenols in different diseases. However, it remains unclear how such effects could be triggered. In order to elucidate the effects of epicatechin gallate (ECG) in C6 cells, both by itself and against H2O2-induced genotoxicity, measurements of DNA strand breaks and chromosome loss were performed. DNA damage was measured by comet and micronucleus assays. The present study shows for the first time how ECG, the major green tea-derived polyphenol, is able to exert dose-dependent genoprotective effects in an H2O2-induced toxicity model of C6 astroglial cells. We demonstrate that doses of ECG in a range from 0.1 to 1μM were able to completely prevent H2O2-induced genotoxicity in vitro. In contrast, considerably higher concentrations of ECG (10μM) were able to reverse previous positive effects in a dose- and time-dependent manner. The same results were confirmed by both comet (F3,9=336,148; P<.001) and micronucleus (F3,9=23,228; P<.001) methods. Together, our data show ECG as a dose-dependent genoprotective compound in C6 astroglial cells. This indicates that small doses of polyphenols included in our diet could have beneficial effects on neural cells, contributing to prevention of oxidative stress-associated brain pathologies. In addition, our data highlight the importance of strictly modulating doses and/or consumption of antioxidant-fortified foods or additional supplements containing such beneficial molecules.
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