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PAL-XFEL soft X-ray scientific instruments and X-ray optics: First commissioning results
Park, Sang Han,Kim, Minseok,Min, Changi-Ki,Eom, Intae,Nam, Inhyuk,Lee, Heung-Soo,Kang, Heung-Sik,Kim, Hyeong-Do,Jang, Ho Young,Kim, Seonghan,Hwang, Sun-min,Park, Gi-Soo,Park, Jaehun,Koo, Tae-Yeong,Kwo American Institute of Physics 2018 Review of scientific instruments Vol.89 No.5
Park Cheol,Cha Hee-Jae,Kim Da Hye,Kwon Chan-Young,Park Shin-Hyung,Hong Su Hyun,Bang EunJin,Cheong Jaehun,Kim Gi-Young,Choi Yung Hyun 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.5
Fisetin is a bioactive flavonol molecule and has been shown to have antioxidant potential, but its efficacy has not been fully validated. The aim of the present study was to investigate the protective efficacy of fisetin on C2C12 murine myoblastjdusts under hydrogen peroxide (H2O2)-induced oxidative damage. The results revealed that fisetin significantly weakened H2O2-induced cell viability inhibition and DNA damage while blocking reactive oxygen species (ROS) generation. Fisetin also significantly alleviated cell cycle arrest by H2O2 treatment through by reversing the upregulation of p21WAF1/CIP1 expression and the downregulation of cyclin A and B levels. In addition, fisetin significantly blocked apoptosis induced by H2O2 through increasing the Bcl-2/Bax ratio and attenuating mitochondrial damage, which was accompanied by inactivation of caspase-3 and suppression of poly(ADP-ribose) polymerase cleavage. Furthermore, fisetin-induced nuclear translocation and phosphorylation of Nrf2 were related to the increased expression and activation of heme oxygenase-1 (HO-1) in H2O2- stimulated C2C12 myoblasts. However, the protective efficacy of fisetin on H2O2-mediated cytotoxicity, including cell cycle arrest, apoptosis and mitochondrial dysfunction, were greatly offset when HO-1 activity was artificially inhibited. Therefore, our results indicate that fisetin as an Nrf2 activator effectively abrogated oxidative stress-mediated damage in C2C12 myoblasts.
Park, Jaeheung,Lee, Taegon,Park, Jaehun,Lim, Manho American Chemical Society 2013 The journal of physical chemistry. B, Condensed ma Vol.117 No.10
<P>Femtosecond vibrational spectroscopy was used to investigate the photoexcitation dynamics of NO-bound ferric myoglobin (Mb<SUP>III</SUP>NO) in D<SUB>2</SUB>O solution at 294 K after excitation with a 575 nm pulse. The stretching mode of NO in Mb<SUP>III</SUP>NO consists of a major band at 1922 cm<SUP>–1</SUP> (97.7%) and a minor band at 1902 cm<SUP>–1</SUP> (2.3%), suggesting that Mb<SUP>III</SUP>NO in room temperature solution has two conformational substates. The time-resolved spectra show small but significant new absorption features at the lower-energy side of the main band (1920–1800 cm<SUP>–1</SUP>). One new absorption feature in the region of 1920–1880 cm<SUP>–1</SUP> exhibits the <SUP>15</SUP>NO isotope shift (37 cm<SUP>–1</SUP>) the same as that of the NO band in the ground electronic state of Mb<SUP>III</SUP>NO. This absorption shifts toward higher energy and narrows with a time constant of 2.4 ps, indicating that it evolves with rapid electronic and thermal relaxation of the photoexcited Mb<SUP>III</SUP>NO without photodeligation of the NO from the heme. Absorption features assigned to proteins undergoing thermal relaxation without NO deligation add up to 14 ± 1% of the total bleach, implying that the photolysis quantum yield of Mb<SUP>III</SUP>NO with a Q-band excitation is ≤0.86 ± 0.01. The remaining absorption bands peaked near 1867, 1845, and 1815 cm<SUP>–1</SUP>, each showing the <SUP>15</SUP>NO isotope shift the same as that of the free NO radical (33 cm<SUP>–1</SUP>), were assigned to the vibrational band of the photodeligated NO, the NO band of Mb<SUP>III</SUP>NO in an intermediate electronic state with low-spin Fe(III)–NO(radical) character (denoted as the R state), and the NO band of the vibrationally excited NO in the R state, respectively. A kinetics model successfully reproducing the time-dependent intensity changes of the transient bands suggests that every rebound NO forms the R state that eventually relaxes into the ground electronic state nonexponentially. Most of the photodissociated NO undergoes fast geminate recombination (GR), and the rebinding kinetics depends on the conformation of the protein. GR of NO to Mb<SUP>III</SUP> in the major conformation shows highly nonexponential kinetics described by a stretched exponential function, exp(−(<I>t</I>/290 ps)<SUP>0.44</SUP>. The NO rebinding to Mb<SUP>III</SUP> in the minor conformation is exponential, exp(−<I>t</I>/1.8 ns), suggesting that the distal histidine, the interaction of which dictates the conformation of Mb<SUP>III</SUP>NO, participates in mediating the binding of NO to Mb<SUP>III</SUP>. In Mb<SUP>III</SUP>NO, the elusive low-spin Fe(III)–NO(radical) state, proposed in electronic structure calculations, indeed exists at >12 kJ/mol above the ground state and takes part in the bond formation of Fe(III)–NO, suggesting that it plays a significant role in the function of NO-bound ferric protein. Time-resolved vibrational spectra with high sensitivity reveal rich photophysical and photochemical processes of photoexcited Mb<SUP>III</SUP>NO.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpcbfk/2013/jpcbfk.2013.117.issue-10/jp400055d/production/images/medium/jp-2013-00055d_0013.gif'></P>
한국뷰티산업(K-Beauty)의 성장과정 고찰과 발전방향에 대한 소고(小考)
박재훈 ( Jaehun Park ),이주희 ( Ju Hee Lee ),김용정 ( Yong Jeong Kim ),송영욱 ( Young Wook Song ) 한국유통물류정책학회 2020 유통물류연구 Vol.7 No.1
한국의 경제발전과 글로벌 한류열풍으로 한국뷰티산업(K-Beauty)에 대한 세계적인 관심도 증가하고 있다. 따라서 본 연구는 시기적으로 그 중요성이 높아지고 있는 뷰티산업을 제조와 서비스 분야로 구분하여 실태를 진단하고 현실성 있는 발전방향을 제시한다. 연구목적에 부합하는 실효성 있는 연구를 위해서 연구방법은 질적인 방법에 의한 연구가 적합할 것으로 판단된다. 따라서 뷰티산업의 현장방문을 통해 화장품 및 미용업체의 전문가를 대상으로 개별 심층인터뷰 (In-depth Interview)를 진행하였다. 연구결과, 화장품제조업 분야의 전문가 인터뷰에서는 해외기업과의 제휴와 다양한 추출기법을 접목하여 독창적인 원료개발, 생산설비 자동화와 공정개선으로 비용절감, 일관된 제품성상의 유지와 품질적합성을 위한 철저한 품질관리, 전문물류업체와의 제휴를 통해 납기의 신뢰성 보장 등이 중요한 것으로 식별되었다. 그리고 미용서비스업 분야의 전문가 인터뷰에서는 종사원 근무환경 미흡, 감정노동, 높은 이직률, 서비스 비즈니스모델로서 수익구조의 취약성, 뷰티산업 성장을 위한 실효성 있는 법률체계 미비와 같은 문제점의 해결을 중요하게 생각하고 있었다. 본 연구의 전문가 인터뷰결과를 활용한다면 정부와 기관, 뷰티업계, 그리고 관련 단체에서 뷰티산업 발전을 위한 실효성 있는 전략방향 수립을 위해 어떠한 부분에 중점을 두어야 하는지에 대한 시사점을 줄 수 있을 것이다. 또한 본 연구는 그동안 학술적으로 연구가 활성화되지 않았던 분야인 뷰티산업을 대상으로 제조와 서비스 부문으로 구분하고 그 성장과정을 고찰하여 시기적으로 부합하는 실효성 있는 발전방향의 토대를 제시하고자 했다는 점에 그 의미가 있다. Korea’s beauty industry is a future growth sector that is evaluated to create high value-added products with a high level of synergy with the national interest aligned with the Korean culture, art and tourism industries. The purpose of this study is to present concrete directions for the development of Korea’s beauty industry fitting the current Korean circumstances. This study succinctly examines the experts’ views on the beauty industry. The research methodology is to select managers from the representative cosmetic and beauty treatment firms in Korea, aims to apply the qualitative research methodology through the site visit, and an in-depth interview with these experts. First of all, the result of cosmetic field shows that the crucial factors are listed such as product creativity, efficacy effect, raw material price, and suitability to specification. It is an overall consensus that the product quality is the most important factor due to cosmetic’s natural features. Branded companies regard creativity as the most important factor from the marketing point of view; thus, it is necessary to offer raw materials with patents and monopolistic supply. The result of beauty treatment field interviews proposes improving employees’ working conditions and implementing institutional devices such as consumption ethics and fair trade rights to protect employees from emotionally-offended labor, and establishing action guidelines and internal regulations to protect employees from customers. Additionally, this study proposes developing programs to manage employees’ level of stress. This study contributes to help grasp key critical issues as it analyzes opinions of beauty industry experts through in-depth interview; as such, the result of the analysis is effectively applicable to the industrial practice. Also, it is timely and meaningful for governmental policy makers in that this research presents the directions of effective developmental strategy.