집파리 불임화를 위한 Flufenoxuron제제의 개발

박정규,강창헌 경상대학교 농업생명과학연구원 2001 농업생명과학연구 Vol.35 No.-

본 연구에서는 불임화제제의 야외적용을 위한 소규모 실험과 제제의 제조 및 효과 검정, 불임화제제의 저장 기간별 약효 지속기간 점검 등을 실험하였다. 소규모 사육상자실험에서는 flufenoxuron 1,500ppm과 500ppm 모두 F_1난의 부화율이 0%로서 아주 우수한 불임효과를 나타내었으며, flufenoxuron 처리는 파리의 유인이나 섭식에 영향이 없는 것으로 나타났다. 불임화제제의 개발과 효과 검정에서는 여러 가지 제제 중에서 펄라이트제제와 톱밥제제가 가장 효과적이었다. 펄라이트를 이용한 불임화제제를 제조하여 실제 우사(牛舍)에서의 집파리에 대한 유인효과를 검정한 결과, 약제의 농도에 따라서는 유인수에 차이가 없고, 집파리의 sex pheromone인 muscamone을 함유시킨 제제의 유인력이 좋았으며, 처리에 따라 유인된 암, 수의 비율에는 차이가 없었다. Perlite 불임화제제를 실내 및 냉장 조건에서 일정기간 저장한 후 불임효과를 검토하였는데, 실혼에 저장시 불임효과가 100% 유지되었다. A series of experiments were conducted to test the efficacy of laboratory-made flufenoxuron(1-[4-(2-chloro-a,a,a-trifluoro-p-tolyloxy)2-fluorophenyl]-3-(2,6-difluoro- benzoyl) urea) formulations for house fly (Musca domestica) sterilization. Sterilization effect of a sugar granule formulation dipped in ethanol solution (1,500 and 500 ppm) of flufenoxuron was tested in rearing cages in laboratory. The eggs deposited by the flies contacted to the sugar granule formulation showed 0% of hatchability. The flufenoxuron itself (20~700 ppm) coated to sugar granules had no effect on the attraction and feeding amount of the flies. Four types of flufenoxuron formulations dipped in 500 ppm solution were tested for its sterilization efficacies against house fly in laboratory. The perlite and sawdust formulations were most effective. The female flies in cages containing sawdust formulation deposited 100% sterilized eggs. Less than 30% of hatchability showed the eggs deposited by the flies in the cages containing the sugar granule and the perlite formulations. The attractancy of perlite formulation was tested in a cattle pen. The formulation was made by dipping perlite carrier in flufenoxuron solutions (100 and 500 ppm) to which house fly sex pheromone(muscamone) was added. Concentration of the flufenoxuron solution in which the perlite were dipped has no effect on the attractancy. The muscamone added formulation attracted significantly more flies than the check treatments. However there were no differences in the sex ratio of the attracted flies among flufenoxuron concentrations and muscamone existence in the formulation. The formulation could be stocked for 10 days in a room without reduction of sterilization efficacy.

단감의 저온저장 기간에 따른 해충의 밀도변화에 관한 연구

강창헌,박정규 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.1

우리나라의 단감의 수출선을 다변화하기 위해서는 검역문제를 해결하여야 한다. 본 논문에서는 저온저장 기간 중에 단감에 존재하는 해충이 저온에 의해서 어느 정도 제거되는지를 조사하였다. 톡톡이류는 약 20일간의 저온(-1±0.5℃)저장에 의해서 밀도가 1/10로 감소하였고, 저장 56일 이후에는 저장 중인 과일에 톡톡이를 찾을 수 없었다. 균식성응애도 저온에 대한 내성이 낮아 저온저장에 의해서 밀도가 현저히 감소하였으며 74일 저장 후에는 거의 찾아볼 수가 없었다. 차응애도 저장 56일 이후에는 전혀 찾아볼 수 없었다. 한편 주머니깍지벌레는 꽃받침 밑에서만 월동 난 상태로 휴면하고 있었는데, 다른 곤충과는 달리 저온에 대한 내성이 강하였다. 저장 74일 이후에도 과일당 5개의 알 수준으로서 다른 충에 비해서 밀도 감소가 뚜렷하지 않았다. 월동난 존재과율도 전 저장기간에 걸쳐 5~13%에 달하였다. 따라서 주머니깍지벌레가 발생하는 포장에서 생산되는 단감의 경우에는 이들을 제거할 수 있는 새로운 기술을 개발해야 할 것이다. Insects or mites on the exporting fruits should not be allowed to meet the quarantine requirements of importing countries. Some fruits are stored before shipment for export at -1±0.5℃. Density changes of the pests on persimmon were checked during the low temperature storage (LTS). The collembolan could be killed by LTS. Density of collembolan under calyx decreased to one-tenth of the initial after a 20-d storage. No collembolan survived after 56-d storage. The LTS resulted in high mortality of the fungus-feeding mite and leaf mite. There were no fungus-feeding and leaf mites on the fruits after a 74- and a 56-d storage, respectively. However the overwintering eggs of grape-myrtle scale (Eriococcus largerstroemiae Kuwana) survived the LTS. Some of them were still alive after a 74-d storage; 5-13% of the sample fruits had the eggs under their calyxes. Therefore the fruits from the orchards infested by E. lagerstroemiae should be treated differently to kill the scales.

단감 방화곤충의 종류와 일주활동에 관한 연구

강창훈,허혜순,박정규 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.3

경남 진주, 사천, 김해 지역의 단감 과수원에서 단감꽃을 방문하는 방화곤충의 종류와 일주활동 리듬을 조사하였다. 단감꽃을 방화하는 곤충은 모두 2목 15종이었으며, 그 중에서 양봉꿀벌이 전체의 73.6%로 우점종이었고, 일본애수염줄벌이 10.2%, Andrena habes와 좀뒤영벌이 각각 3.0%를 차지하였다. 우점종인 양봉꿀벌은 13:00부터 15:00까지 가장 활발하게 활동하였다. Insect visitors were surveyed on sweet persimmon blossoms at Jinju, Sacheon, and Gimhae in Gyeongnam province, 2001. Fifteen species in 2 orders were collected. Honeybee, Apis mellifera, was a dominant species representing 73.6% and followed by Tetralonia nipponensis(10.2%), Andrena habes and Bombus ardens ardens(3.0%) in turn. Foraging of honeybee was most active between 13:00 to 15:00.

경남지역의 채소류와 화훼류 해충에 관한 조사

신현열,이동운,추호렬,박정규 경상대학교 농업생명과학연구원 2001 농업생명과학연구 Vol.35 No.-

본 연구는 1996년 경남지역 주요 채소류와 화훼류 작물에 발생하는 해충과 기타 주요 해로운 동물의 종류를 알아보기 위하여 수행하였다. 오이에서는 오이총채벌레와 꽃노랑총채벌레를 포함하여 7과 8속 10종이 확인되었으며, 양파에서는 알톡토기를 포함한 4과 4속 4종이 조사되었고, 배추에서는 배추좀나방과 담배거세미나방을 포함한 10과 13속 14종이 확인되었다. 거베라에서는 아메리카잎굴파리와 점박이응애, 담배거세미나방을 포함한 8과 8속 10종이, 글라디올러스에서는 명주달팽이와 민달팽이, 들민달팽이를 포함하여 6과 7속 8종이, 국화에서는 민달팽이와 꽃노랑총채벌레를 포함한 10과 13속 14종의 해충이 확인되었다. Insect pests were surveyed on the major vegetable and floricultural crops in Gyeongnam province in 1996. In addition, some other important animal pests were included in this survey. Ten species of 8 genera in 7 families including Thrips palmi and Frankliniella occidentalis were found from cucumber; 4 species of 4 genera in 4 families including Bourletiella hortensis from onion; 15 species of 14 genera in 11 families including Plutella xylostella and Spodoptera litura from Chinese cabbage; 10 species of 8 genera in 8 families including Liriomyza trifolii, Tetranychus urticae and S. litura from gerbera; 6 species of 7 genera in 8 families including Acusta despecta, Incilaria contusa and Deroceras varians from gladiolus; and 14 species of 13 genera in 10 families including I. contusa and F. occidentalis from chrysanthemum.

단감 수학과에 잔존하는 절지동물의 제거기술 평가

강창훈,이상명,허혜순,박정규 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.4

우리나라 단감의 수출선을 다변화하기 위해서는 검역문제를 해결하여야 한다. 본 연구는 이러한 문제를 해결하기 위하여 단감 수확과에 잔존하는 해충의 제거방법으로 고온처리와 air shower 처리의 효과를 실험하였다. 점박이응애를 단감(부유)에 접종하고 50℃에서 4시간 처리하면 99.4%의 치사효과를 얻을 수 있었지만, 45℃에서 3시간이나 50℃에서 1시간 이상 처리하였을 때 감이 연화되는 현상이 나타났다. 단감의 수확과에 존재하는 충은 톡토기류, 균식성응애, 잎응애의 3종류이었는데 균식성응애의 밀도가 가장 높았다. 꽃받침 윗면에 존재하는 충은 100% 제거할 수 있었다. 그러나 아랫면에 존재하는 충은 톡토기와 잎응애는 air shower로 100과 중에서 2마리 수준까지 제거할 수 있었지만 균식성응애는 원래 밀도가 높아서 모두 제거하는 데는 무리가 있었다. 따라서 수확과에 잔존하는 해충을 완전히 제거할 수 있는 새로운 기술의 개발이 요구된다. No quarantine pests are allowed in exporting fruits. To meet the quarantine requirement, it is essential to remove pests from harvested fruits. We tested a few methods to remove or kill the pests on persimmon fruits. The efficacies of hot-air treatment and air shower were tested on sweet persimmon. Hot air (50℃) treatment for 4 h showed 99.4% mortality of Tetranychus urtioae. However, longer than 3-h treatment at 45℃ and 1-h treatment at 50℃ caused softening of the fruits. The efficacy of air shower generated with a compressor (max W.P., 200 lbs; 10-14 sec shower/fruit) in removing the pests on persimmon fruits was tested. Collembolans, fungus-feeding mites, and leaf mites on and under the calyx lobes were the common pests on persimmon fruits. All the pests on the calyx could be removed by the air shower treatment. However the pests under the calyx were resistant to the air shower treatment. Collembolans and leaf mites could be reduced to two individuals per 100 fruits (98% removal). However, the fungus-feeding mites could not be removed by the air shower because of the high initial density of the mite and the shield by the calyx. Therefore it is evident that the air shower treatment cannot remove 100% of the pests under the calyx. Therefore development of new technology is required to remove pests from harvested persimmon fruits.

Chemical Communications of Insect Pests and Natural Enemies at Persimmon Ecosystem

Chung gyoo Park,Junheon Kim,Hyun sik Roh 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Chemical communication is very important for survival and reproduction in insects. The successful survival of insects depends to a large extent on the ability to detect and recognize environmental signals. We can manage the insect pest populations by eco-friendly methods by understanding the chemical communication systems which lie among the host trees, pests, and natural enemies. Persimmon is the largest fruit tree in acreage in Korea. Many insect pests are attacking various part of persimmon. Major insect pests of persimmon in Korea are Riptortus pedestris, Halyomorpha halys, Plautia stali as hemipterans, Stathmopoda masinissa, Euzophera batangensis, Conogethes punctiferalis as lepidopterans, There are also some minor insect pests including Homona magnanima, Apolygus spinolae, Synanthedon tenuis, bark beetles et cetera. Some of these produce sex pheromones to attract conspecific females or produce aggregation pheromones to gather conspecific individuals. Interestingly, some insect pests are using the semiochemicals of other species, pheromones or floral scents, as kairomones to find hosts or oviposition sites. These relationships in chemical communications at persimmon ecosystem will be discussed using our research results and others so far.

Accumulation of Dietary Conjugated Linoleic Acid (CLA) in Silkworm, Bombyx mori

Chung-Gyoo Park,Seck-Jong Kim,Nam-Gue Ha,Bong-Il Choi,Yeong-Lae Ha 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.1

Conjugated linoleic acid (CLA) exhibits potent anti-carcinogenic and other biological activities in several animal models. We report here that dietary CLA, chemically synthesized from corn oil, accumulates in silkworm, Bombyx mori, which is used as a therapeutic agent for diabetes in Korea and Japan. Mulberry leaves treated with 0.1 or 10% CLA in ethanol were supplied to silkworms from the end of the 3rd instar to the 3rd day of the 5th instar. Fresh mulberry leaves or leaves treated with 10% corn oil in ethanol were fed as a check treatment. The amount of total lipids in the larval body ranged from 17.4 to 19.1 ㎎/g of body tissue, which was not significantly affected by the source of the diets. No CLA was found in the control silkworms. But the level of CLA significantly increased to 83.5 ㎎/g of fat, when fed with mulberry leaves treated with 10% CLA. Only trace amounts of CLA were detected in the larvae reared with check leaves and 0.1% CLA-treated leaves. Mulberry leaves treated with corn oil or CLA were not palatable to the larvae, resulting in a reduction of larval weight. These results suggest that silkworms containing CLA in body lipids could be produced by dietary CLA.

Accumulation of Dietary Conjugated Linoleic Acid (CLA) in Silkworm, Bombyx mori

Park, Chung-Gyoo,Kim, Seck-Jong,Ha, Nam-Gue,Park, Bong-Il,Ha, Yeong-Lae The Korean Society of Food Science and Nutrition 2001 Preventive Nutrition and Food Science Vol.6 No.1

Conjugated linoleic acid (CLA) exhibits potent anti-carcinogenic and other biological activities in several animal models. We report here that dietary CLA, chemically synthesized from corn oil, accumulates I silkworm, Bombyx mori, which is used as therapeutic agent for diabetes in Korea and Japan. Mulberry leaves treated with 0.1 or 10% CLA in ethanol were supplied to silkworms from the end of 3rd instar to the 3rd day of the 5th instar. Fresh mulberry leaves or leaves treated with 10% corn oil in ethanol were fed as a check treatment. the amount of total lipids in the larval body ranged from 17.4 to 19.1mg/g of body tissue, which was not significantly affected by the source of the diets. No CLA was found in the control silkworms. But the level of CLA significantly increased to 83.5 mg/g of fat, when fed with mulberry leaves treated with 10% CLA. Only trace amounts of CLA were detected in the larvae reared with check leaves and 0.1% CLA-treated leaves. Mulberry leaves treatd with corn oil or CLA were not palatable to the larvae, resulting in a reduction of larval weight. These results suggest that silkworms containing CLA in body lipids could be producted by dietary CLA.

Insect Chemical Communication & Its Application to Insect Pest Control

Chung Gyoo PARK(박정규),Tae Hyung KWON 한국농약과학회 2017 한국농약과학회 학술발표대회 논문집 Vol.2017 No.11

Development of Phytosanitary Treatment using Ethyl formate for Harvested Sweet Persimmon

Chung Gyoo Park,Jin Hoon Cho,Byung-Ho Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

This study was done to evaluate the efficacy of Vapormate (ethyl formate 16.7 % + CO2) as an methyl bromide-alternative fumigant. Some insect pests and mites were found under calyx of sweet persimmon fruits, including grape-myrtle scale, two-spotted spider mite, Amblyseius eharai. Vapormate fumigation was equally toxic to miticide- susceptible and -resistant T. urticae showing 100 % mortality at higher concentration than 146.7 and 211.0 mg/L, respectively. In persimmon-filled desiccator, summer and overwintering types of T. urticae showed 100 % mortality at higher concentration than 163.7 and 265.8 mg/L, respectively. Sweet persimmon damage from vapormate fumigation was not shown by the days after treatment. Tolerance of each live stage of grape-myrtle scale was in descending order: egg < nymph < adult. EF 141.5 mg h L-1 for 6 h at 5 ℃ killed 100 % of the larvae of D. punctiferalis. The final scaled-up fumigation confirmed the efficacy of EF to target pests and non-phytotoxicity on persimmons. In conclusion, EF may become a MB alternative fumigant for sweet persimmon, and the LC99 values of EF may be used to practical applications to all kinds of insects and mites on harvested sweet persimmon.