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- 저자 : Pariza, Michael W. (검색결과 5 건)
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Conjugated Linoleic Acid(CLA)의 암세포 증식 억제효과 및 Interleukin - 1과 Interleukin - 2의 생성에 미치는 영향
김소희(So-Hee Kim),김광혁(Kwang-Hyuk Kim),박건영(Kun-Young Park),Michel W. Pariza(Michael W. Pariza) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.5
육류 및 낙농제품을 비롯하여 많은 자연식품에 함유되어 있으며 사람의 체내에도 존재하는 것으로 알려진 CLA가 암세포 증식에 미치는 효과와 면역계에서 생물학적 작용에 관여하는 cytokine인 IL-1과 IL-2의 생성에 미치는 영향을 흉선세포와 CTLL-2 세포주를 이용한 bioassay 방법으로 검토하였다. CLA는 강한 암세포 증식억제효과를 나타내어 ml당 50㎍의 농도에서 마우스의 림프종 세포인 Yac-1 세포의 증식을 98%이상 억제시켰으며 복수암 세포인 sarcoma-180 세포의 증식도 ml당 125㎍의 농도에서 80% 이상 억제시켰다. 또한 CLA는 농도(31, 63, 125, 250㎍/ml) 의존적으로 비장 세포의 IL-1 생성을 억제시키는 결과를 보여 CLA가 항염증작용을 가지는 성분이 될 수 있음을 시사하였다. 뿐만 아니라 CLA는 모든 실험 농도(31, 63, 125, 250㎍/ml)에서 IL-2의 생성을 억제시켰다. 따라서 CLA가 암세포 증식에 미치는 효과는 IL-1과 IL-2와 같은 사이토 카인의 생성 증진에 따른 면역 담당 세포의 활성화가 아닌 암세포의 단백질 및 핵산의 생합성 저해 등과 같은 작용과 관련을 가질 가능성이 있을 것으로 생각된다. Conjugated dienoic derivatives of linoleic acid(CLA) are a series of positional and geometric isomers of linoleic acid which are found naturally in food, mainly dietary products and beef. We studied the effects of CLA on the growth of tumor cells and the production of interleukin -1 (IL-1) and interleukin-2(IL-2). CLA treatment markedly inhibited the growth of Yac-1 cells and sarcoma-180 cells by 99 and 82% to that of control, respectively, after four days of incubation at 37℃. To elucidate the immunological mechanism of antitumor activity of CLA, spleen cells of Balb/c mouse were exposed to 31, 63, 125, 250 ㎍ of CLA per ml for 24hrs at 37℃. The culture supernatants of CLA-exposed spleen cells reduced the production of IL-1 and IL-2 in all of the test conditions. These results indicate that the anticarcino-genic effect of CLA was mediated by the other actions rather than the production of the IL-1 or IL-2. We suggest that CLA might have an antiinflammatory effect in part due to its inhibitory action on the production of IL-1.
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Bioactivities and Potential Mechanisms of Action for Conjugated Fatty Acids
Yeonhwa Park,Michael W. Pariza 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3
Since conjugated linoleic acid (CLA) was identified as a principal anticancer component from ground beef in the 1980s, CLA research has discovered that CLA has a wide range of biologically beneficial effects. Clinical studies with CLA are on the rise, and it is apparent that CLA may not be as effective in humans as in rodents, in particular its anti-obesity aspect. In addition, research with regard to other conjugated fatty acids as well as CLA metabolites is still in its infancy. Investigation of bioactivities for other conjugated fatty acids and CLA metabolites may help to extend the understanding of CLA and its mechanisms of actions. This may pose an opportunity to use CLA more efficiently and expand the future use of other conjugated fatty acids as pharmacological agents to assist current treatments.
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새로이 分類된 天然 抗癌劑 : Conjugated Dienoic Derivatives of Linoleic Acid (CLA)
하영래(Yeong L. Ha),마이클 파리자(Micheal W. Pariza) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.4
동물실험을 통하여 새로이 밝혀진 항암제(anti-initiator/anti-ptomotor)인 CLA는 grilled ground beef에서 처음 분리되었다. CLA는 grilled ground beef외에도, cheese 및 이들 관련식품에 많이 존재한다. CLA는 반추동물의 위에 서식하는 혐기성 bacteria에 의해 linoleic acid로부터 생성되며, 식품 가공 중에서도 생성된다. 이것은 또한 in vivo에서 linoleic acid의 carbon centered free radical 형태의 산화에 의해 생성되기도 한다. CLA는 아주 강력한 항산화제임이 밝혀져, 지금까지 알려져 있지 않았는 free radical에 대응하여 membrane을 보호하는 in situ defense mechanism 역할을 한다. 이는 또한 cytochrome P450 isozyme의 활성을 저해하는 반면, ODC 효소 활성 역시 저해한다. 그래서, 적어도 CLA의 이 세가지 biological activity가 CLA 항암기작에 관여하는 것으로 생각된다. Anticarcinogenic conjugated dienoic derivatives of linoleic acid(CLA) is present in grilled beef, cheese, and related foods. CLA is generated via isomerization of linoleic acid in the cow's rumen by anaerobic bacteria and food proceessing as well. Another source of CLA is its endogenous generation via the carbon centered free radical oxdation of linoleic acid. We propose that the formation and generation of CLA in vivo represents a previously unrecognized in situ “defense mechanism” against membrane attack by oxygen free radicals. The cis, 9-trans, 11 CLA isomer is selectively incorporated into cellular phospholipid, which exhibits a potent antioxidant, reduces the activation of 2-amino-3-methylimidazo 〔4, 5-f〕 quinoline (IQ) for bacterial mutagenesis, and inhibits ornithine decarboxylase(ODC) activity induced by 12-0-tetradecanoylphorbol-13-acetate (TPA). We believe that at least these three biological activities of CLA explain the anticarcinogenic activity of CLA.
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Kyung-Ah Park,Seck-Jong Kim,Sook-Jahr Park,Gu-Boo Park,Dong-Kil Lim,Kyeong-Nyeo Bahn,Yong-Un Cho,Jung H.Y. Park,Michael W. Pariza,Yeong-Lae Ha 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.1
Conjugated linoleic acid (CLA), when incorporated into mouse liver microsomal membranes, selectively inhibits the mutagenesis of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ). Nine-week old female ICR mice were given (p.o.) 0.1 mL olive oil alone (control), 0.1 mL olive oil plus 0.1 mL linoleic acid, or 0.1 mL olive oil plus 0.1 mL CLA, twice weekly for four weeks. The animals were then sacrificed and liver S-9 fractions were prepared. Activation of IQ for mutagenesis by the liver S-9 from CLA-treated mice was significantly reduced in comparison with liver S-9 from control or linoleic acid-treated mice. By contrast, the activation of 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]pyrene (BP) was unaffected. Hence, CLA incorporated into phospholipids may selectively affect cytochrome P450 isozymes responsible for activating IQ, but not those which activate BP or DMBA. The addition of free CLA or the methyl esters of CLA, linoleic acid, or oleic acid, to control S-9 inhibited the activation of all three mutagens (IQ, BP, and DMBA).
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Park, Kyung-Ah,Kim, Seck-Jong,Park, Soo-Jahr,Park, Gu-Boo,Lim, Dong-Kil,Bahn, Kyeong-Nyeo,Cho, Yong-Un,Park, Jung H.Y.,Pariza, Michael W.,Ha, Yeongl-Lae The Korean Society of Food Science and Nutrition 2001 Preventive Nutrition and Food Science Vol.6 No.1
Conjugated linoleic acid (CLA), when incorporated into mouse liver microsomal membranes, selectively inhibits the mutagenesis of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ). Nine-week old female ICR mice were given (p.o.) 0.1 mL olive oil alone (control), 0.1 mL olive oil plus 0.1 mL linoleic acid, or 0.1 mL olive oil plus 0.1 mL CLA, twice weekly for four weeks. The animals were then sacrificed and liver S-9 fractions were prepared. Activation of IQ for mutagenesis by the liver S-9 from CLA-treated mice was significantly reduced in comparison wit liver S-9 from control or linolic acid-treated mice. By contrast, the activation of 7,12-dimethylbenz[a] anthracene (DMBA) and benzo[a] pyrene (BP) was unaffected. Hence, CLA incorporated into phospholipids may selectively affect cytochrome P450 isozymes responsible for activating IQ, but not those which activate BP or DMBA. The addition of free CLA or the methyl esters of CLA, linoleic acid, or oleic acid, to control S-9 inhibited the activation of all three mutagens (IQ, BP, and DMBA).
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