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Juan Pablo Quintal-Martínez,Irma Guadalupe Quintal-Ortiz,Ligia Gabriela Alonzo-Salomón,David Muñoz-Rodríguez,Maira Rubi Segura-Campos 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.12
In Mexico, Cnidoscolus aconitifolius (chaya) has been used to treat cardiovascular diseases (CVD). Because CVD are the number one cause of mortality, chaya use has become a health strategy. The aim of this study was to evaluate the antithrombotic activity and identify the metabolites in the most active extract. Aqueous (Aq), ethanolic (EtOH), acetonic (An), ethyl acetate (AcOEt), diethyl ether (Et2O), and hexanic (Hx) extracts were obtained. Platelet aggregation, phospholipase A2, prothrombin time (PT), activated partial thromboplastin time (aPTT), and clot lysis were evaluated. Metabolites were identified by gas chromatography–mass spectrometry (GC-MS). EtOH showed the greatest inhibition of platelet aggregation and phospholipase A2. Ac had the greatest effect on PT and aPTT. AcOEt had the greatest effect on clot lysis. EtOH, with the highest potential, was analyzed by GC-MS; fatty acids and triterpenes were identified. Thus, EtOH showed greater antiplatelet activity and other extracts showed moderate activity. This is a preliminary antithrombotic study. Future research will allow the development of nutraceuticals or functional ingredients for the prevention and treatment of thrombosis.
Hernandez-Ortiz, Juan P.,Ma, Hong-Bo,de Pablo, Juan J.,Graham, Michael D. The Korean Society of Rheology 2008 Korea-Australia rheology journal Vol.20 No.3
Simulations of solutions of flexible polymer molecules during flow in simple or complex confined geometries are performed. Concentrations from ultradilute up to near the overlap concentration are considered. As concentration increases, the hydrodynamic migration effects observed in dilute solution unidirectional flows (Couette flow, Poiseuille flow) become less prominent, virtually vanishing as the overlap concentration is approached. In a grooved channel geometry, the groove is almost completely depleted of polymer chains at high Weissenberg number in the dilute limit, but at finite concentration this depletion effect is dramatically reduced. Only upon inclusion of hydrodynamic interactions can these phenomena be properly captured.
Juan P. Hernandez-Ortiz,Hongbo Ma,Juan J. de Pablo,Michael D. Graham 한국유변학회 2008 Korea-Australia rheology journal Vol.20 No.3
Simulations of solutions of flexible polymer molecules during flow in simple or complex confined geometries are performed. Concentrations from ultradilute up to near the overlap concentration are considered. As concentration increases, the hydrodynamic migration effects observed in dilute solution unidirectional flows (Couette flow, Poiseuille flow) become less prominent, virtually vanishing as the overlap concentration is approached. In a grooved channel geometry, the groove is almost completely depleted of polymer chains at high Weissenberg number in the dilute limit, but at finite concentration this depletion effect is dramatically reduced. Only upon inclusion of hydrodynamic interactions can these phenomena be properly captured.
Alain Tundidor-Camba,Claudio A. Terraza,Luis H. Tagle,Deysma Coll,Pablo Ortiz,Germán Pérez,Ignacio A. Jessop 한국고분자학회 2017 Macromolecular Research Vol.25 No.3
Aromatic polyimides (PIs) containing a cyclopropylamide moiety as bulky polar pendant group were prepared in high yield and were structurally characterized. Polyimides containing 4,4’-hexafluoroisopropyliden diphthalic anhydride and bis(3,4-dicarboxyphenyl)dimethylsilane anhydride were soluble in aprotic polar organic solvents, while those PI derived from benzophenone-3,3′,4,4′-tetracarboxylic dianhydride with a keto central unit was insoluble. The relationship between bulky pendant group and the nature of the central moiety of the dianhydride monomer respect to the thermal properties of the PIs was studied. All PIs were thermally stable showing thermal decomposition temperature with the 10% weight loss (TDT10%) between 425-480 ºC. The glass transition temperature (Tg) values range between 265 and 315 oC. Mechanical properties from films of a selected PI were tested too.
Jonay Poveda,Ana B Sanz,Susana Carrasco,Marta Ruiz-Ortega,Pablo Cannata-Ortiz,Maria D Sanchez-Niño,Alberto Ortiz 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Acute kidney injury (AKI) is characterized by tubular cell death and interstitial inflammation. TWEAK promotes experimental kidney injury and activates the transcription factor NF-κB, a key regulator of genes involved in cell survival and inflammatory response. In search of potential therapeutic targets for AKI, we compared a transcriptomics database of NF-κB-related genes from murine AKI-kidneys with a transcriptomics database of TWEAK-stimulated cultured tubular cells. Four out of twenty-four (17%) genes were significantly upregulated (false discovery rate, FDRo0.05), while nine out of twenty-four (37%) genes were significantly upregulated at FDR o0.1 in both databases. Bcl3 was the top upregulated NF-κB-related gene in experimental AKI and one of the most upregulated genes in TWEAK-stimulated tubular cells. Quantitative reverse transcription PCR (qRT-PCR), western blot and immunohistochemistry confirmed Bcl3 upregulation in both experimental conditions and localized increased Bcl3 expression to tubular cells in AKI. Transcriptomics database analysis revealed increased Bcl3 expression in numerous experimental and human kidney conditions. Furthermore, systemic TWEAK administration increased kidney Bcl3 expression. In cultured tubular cells, targeting Bcl3 by siRNA resulted in the magnification of TWEAK-induced NF-κB transcriptional activity, chemokine upregulation and Klotho downregulation, and in the sensitization to cell death induced by TWEAK/TNFα/interferon-γ. In contrast, Bcl3 overexpression decreased NF-κB transcriptional activity, inflammatory response and cell death while dampening the decrease in Klotho expression. In conclusion, Bcl3 expressed in response to TWEAK stimulation decreases TWEAK-induced inflammatory and lethal responses. Therefore, therapeutic upregulation of Bcl3 activity should be explored in kidney disease because it has advantages over chemical inhibitors of NF-κB that are known to prevent inflammatory responses but can also sensitize the cells to apoptosis.
Alvarado-Ruiz, Liliana,Martinez-Silva, Maria Guadalupe,Torres-Reyes, Luis Alberto,Pina-Sanchez, Patricia,Ortiz-Lazareno, Pablo,Bravo-Cuellar, Alejandro,Aguilar-Lemarroy, Adriana,Jave-Suarez, Luis Feli Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3
HOX transcription factors are evolutionarily conserved in many different species and are involved in important cellular processes such as morphogenesis, differentiation, and proliferation. They have also recently been implicated in carcinogenesis, but their precise role in cancer, especially in cervical cancer (CC), remains unclear. In this work, using microarray assays followed by the quantitative polymerase chain reaction (qPCR), we found that the expression of 25 HOX genes was downregulated in CC derived cell lines compared with non-tumorigenic keratinocytes. In particular, the expression of HOXA9 was observed as down-modulated in CC-derived cell lines. The expression of HOXA9 has not been previously reported in CC, or in normal keratinocytes of the cervix. We found that normal CC from women without cervical lesions express HOXA9; in contrast, CC cell lines and samples of biopsies from women with CC showed significantly diminished HOXA9 expression. Furthermore, we found that methylation at the first exon of HOXA9 could play an important role in modulating the expression of this gene. Exogenous restoration of HOXA9 expression in CC cell lines decreased cell proliferation and migration, and induced an epithelial-like phenotype. Interestingly, the silencing of human papilloma virus (HPV) E6 and E7 oncogenes induced expression of HOXA9. In conclusion, controlling HOXA9 expression appears to be a necessary step during CC development. Further studies are needed to delineate the role of HOXA9 during malignant progression and to afford more insights into the relationship between downmodulation of HOXA9 and viral HPV oncoprotein expression during cercical cancer development.