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Genome-wide Analysis of Iron-Regulated Transporter 1 (IRT1) Genes in Plants
Recep Vatansever,Ertugrul Filiz,Ibrahim Ilker Ozyigit 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.4
Iron (Fe) is an essential micronutrient required in a number of biological processes in plant species. Fe transporters are a type of broad-range metal transporter and have different families functioning in different compartments. This study focused on iron-regulated transporter 1 (IRT1), which are mainly responsible for Fe uptake from root, in 17 selected plant species with an emphasis on Brachypodium distachyon, Chlamydomonas reinhardtii, Solanum lycopersicum and Populus trichocarpa species. All IRT1 proteins were observed to belong to the ZIP (PF02535) protein family with eight transmembrane (TM) domains, and have a similar molecular weight (33.86-42.72 kDa, except for C. reinhardtii with 65.83 kDa) and amino acid length (324-408 aa, except for C. reinhardtii with 639 aa), with pI values of 5.31-7.16. The sub-cellular localization of these proteins was predicted to be the plasma membrane. Similar exon numbers were also detected with most genes having 2-3, except for C. reinhardtii (5), Physcomitrella patens (5) and Vitis vinifera (4). In a phylogenetic tree, monocot-dicot separation was not observed in main groups but some subgroups included only monocot or dicot proteins. Predicted interaction partner analysis of AtIRT1 (AT2G30080.1) pointed to main interaction partners either directly related with iron transport or that of other metal ion. The results of this study provide a theoretical reference for elucidating the structural and biological role of IRT1 genes/proteins in plant species.
Non linear vibrations of stepped beam systems using artificial neural networks
Bagdatli, S.M.,Ozkaya, E.,Ozyigit, H.A.,Tekin, A. Techno-Press 2009 Structural Engineering and Mechanics, An Int'l Jou Vol.33 No.1
In this study, the nonlinear vibrations of stepped beams having different boundary conditions were investigated. The equations of motions were obtained by using Hamilton's principle and made non dimensional. The stretching effect induced non-linear terms to the equations. Natural frequencies are calculated for different boundary conditions, stepped ratios and stepped locations by Newton-Raphson Method. The corresponding nonlinear correction coefficients are also calculated for the fundamental mode. At the second part, an alternative method is produced for the analysis. The calculated natural frequencies and nonlinear corrections are used for training an artificial neural network (ANN) program which has a multi-layer, feed-forward, back-propagation algorithm. The results of the algorithm produce errors less than 2.5% for linear case and 10.12% for nonlinear case. The errors are much lower for most cases except clamped-clamped end condition. By employing the ANN algorithm, the natural frequencies and nonlinear corrections are easily calculated by little errors, and the computational time is drastically reduced compared with the conventional numerical techniques.
Comparison of different bronchial closure techniques following pneumonectomy in dogs
Hakan Salci,A. Sami Bayram,Ozgur Ozyigit,Cengiz Gebitekin,O. Sacit Gorgul 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.4
The comparison of the histologic healing and bronchopleural fistula (BPF) complications encountered with three different BS closure techniques (manual suture, stapler and manual suture plus tissue flab) after pneumonectomy in dogs was investigated for a one-month period. The dogs were separated into two groups: group I (GI) (n = 9) and group II (GII) (n = 9). Right and left pneumonectomies were performed on the animals in GI and GII, respectively. Each group was further divided into three subgroups according to BS closure technique: subgroup I (SGI) (n = 3), manual suture; subgroup II (SGII) (n = 3), stapler; and subgroup III (SGIII) (n = 3), manual suture plus tissue flab. The dogs were sacrificed after one month of observation, and the bronchial stumps were removed for histological examination. The complications observed during a one-month period following pneumonectomy in nine dogs (n = 9) were: BPF (n = 5), peri-operative cardiac arrest (n = 1), post-operative respiratory arrest (n = 1), post-operative cardiac failure (n = 1) and cardio-pulmonary failure (n = 1). Histological healing was classified as complete or incomplete healing. Histological healing and BPF complications in the subgroups were analyzed statistically. There was no significant difference in histological healing between SGI and SGIII (p = 1.00; p > 0.05), nor between SGII and SGIII (p = 1.00; p > 0.05). Similarly, no significant difference was observed between the subgroups in terms of BPF (p = 0.945; p > 0.05). The results of the statistical analysis indicated that manual suture, stapler or manual suture plus tissue flab could be alternative methods for BS closure following pneumonectomy in dogs.