http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Otsuki Masayuki (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Cis-element architecture of Nrf3–sMaf heterodimer binding sites and its relation to diseases
Akihito Otsuki,Masayuki Yamamoto 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.3
Cellular detoxication is essential for healthbecause it provides protection against various chemicalsand xenobiotics. The KEAP1–NRF2 system is important forcellular defense against oxidative and electrophilic stressesas NRF2 activates the transcription of an array of cytoprotectivegenes, including drug-metabolizing and antioxidantenzymes, in a stress-dependent manner. The CNC family oftranscription factors, including NRF2, form heterodimerswith small Maf (sMaf) proteins and bind to consensus DNAsequences that have been referred to as antioxidant responseelement, electrophile response element, or NF-E2-bindingelement. These sequences are now collectively called CNC–sMaf binding element (CsMBE). In addition to forming aheterodimer with CNC proteins, sMaf proteins can formhomodimers and recognize regulatory motifs called Maf recognitionelement (MARE). Although the CsMBE sequencesubstantially overlaps with that of MARE, the sequencesdiffer. NRF2 selectively recognizes CsMBE, which is criticalfor cytoprotection. Recent advances in high-throughputsequencing and population-scale genome analysis providenew insights into the transcriptional regulation involved inthe stress response. The integration of a genome-wide mapof NRF2 occupancy with disease-susceptibility loci revealsthe associations between polymorphisms in CsMBE and diseaserisk, information useful for the personalized medicineof the future.
-
Synthetic Study of Carbohydrate Binding Domain of Siglec7
Akihisa Otsuki,Ryo Okamoto,Masayuki Izumi,Yasuhiro Kajihara 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Sialic acid-binding immunoglobulin-like lectin (Siglec) 7 is one of the sialic acid binding protein that plays an important role in immune system. Siglec 7 is expressed on natural killer cells and displays a unique ligand binding properties comparing to the other members of the Siglec family, specifically binds to 2,8-disialyl residues. The precise structural analysis of Siglec 7 has been already performed, and amino acid residues involved in the ligand binding are reported. In such context, we set out the first total chemical synthesis of Siglec 7 carbohydrate binding domain (CBD) which consists of 127 amino acid residues. Since chemical synthesis can easily introduce unnatural amino acids, we envisioned that we could produce an artificial sialic acid binding lectin based on this chemical synthetic strategy. The synthesis of Siglec 7 CBD was carried out by convergent total chemical synthesis. The whole polypeptide was assembled from 5 peptide segments (Figure), which were synthesized by conventional solid phase peptide synthesis. We applied native chemical ligation (NCL) for the coupling of these segments sequentially from C-terminus, combined with alkylation with haloacetoamide or desulfuration reaction. These reactions can provide pseudo-Gln residue or Ala residue from Cys residues at the ligation sites. Based on these strategies we have successfully obtained the full-length polypeptide of Siglec 7 CBD and currently are investigating the in vitro folding step.
-
Nassar Mohannad,Hiraishi Noriko,Islam Md. Sofiqul,Romero Maria JRH.,Otsuki Masayuki,Tagami Junji 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4
Objectives: Phytic acid (IP6), a naturally occurring agent, has been previously reported as a potential alternative to ethylenediaminetetraacetic acid (EDTA). However, its effect on adhesion to sodium hypochlorite (NaOCl)-treated dentin and its interactions with NaOCl have not been previously reported. Thus, in this study, the effects of IP6 on resin adhesion to NaOCl-treated dentin and the failure mode were investigated and the interactions between the used agents were analyzed. Materials and Methods: Micro-tensile bond strength (µTBS) testing was performed until failure on dentin treated with either distilled water (control), 5% NaOCl, or 5% NaOCl followed with chelators: 17% EDTA for 1 minute or 1% IP6 for 30 seconds or 1 minute. The failed specimens were assessed under a scanning electron microscope. The reaction of NaOCl with EDTA or IP6 was analyzed in terms of temperature, pH, effervescence, and chlorine odor, and the effects of the resulting mixtures on the color of a stained paper were recorded. Results: The µTBS values of the control and NaOCl with chelator groups were not significantly different, but were all significantly higher than that of the group treated with NaOCl only. In the failure analysis, a distinctive feature was the presence of resin tags in samples conditioned with IP6 after treatment with NaOCl. The reaction of 1% IP6 with 5% NaOCl was less aggressive than the reaction of the latter with 17% EDTA. Conclusions: IP6 reversed the adverse effects of NaOCl on resin-dentin adhesion without the chlorine-depleting effect of EDTA.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
