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RISS 인기검색어
- 검색키워드
- 저자 : Omar Asmaa S. M. (검색결과 2 건)
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Nagwa S. M. Aly,김혜숙,Maysa A. Eraky,Asmaa A. El Kholy,Basma T. Ali,Shin-Ichi Miyoshi,Rabab E. Omar 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.3
Schistosomiasis causes significant morbidity and mortality worldwide. This study aimed to assess the effect of schistosomula lung antigen preparation (SLAP) and soluble egg antigen (SEA) on a murine schistosomiasis mansoni model. Ninety laboratory-bred male Swiss albino mice were divided into 6 groups. Two doses of the vaccine were given at 2-week intervals. All mice were subcutaneously infected with 80±10 Schistosoma mansoni cercariae 2 weeks after the last vaccination dose. They were sacrificed 7 weeks post-infection. Parasitological and histopathological studies were conducted to assess the effect of inoculated antigens (single or combined). The results showed that the combination of SLAP and SEA (combination group) led to a significant reduction in worm burden (65.56%), and liver and intestine egg count (59% and 60.59%, respectively). The oogram pattern revealed a reduction in immature and mature eggs (15±0.4 and 10±0.8, respectively) and an increased number of dead eggs in the combination group (P<0.001). In terms of histopathological changes, the combination group showed notably small compact fibrocellular egg granuloma and moderate fibrosis in the liver. A high percentage of destroyed ova was observed in the intestine of the combination group. This study demonstrates for the first time the prophylactic effect of combined SLAP and SEA vaccine. The vaccine induced a significant reduction in the parasitological and pathological impacts of schistosomiasis mansoni in hepatic and intestinal tissues, making it a promising vaccine candidate for controlling schistosomiasis.
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Belal B. E. A.,El Kenawy M. A.,Omar Asmaa S. M. 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6
The color of 'Crimson Seedless' berries is a major constraint in expanding marketing and export potential. The target of this investigation is to enhance the color and quality of 'Crimson Seedless' by using brassinolide (BL) and girdling treatments, individually and in combination, as an alternative to ethephon over two consecutive seasons. The obtained results revealed that the combined treatment BL at 2.0 mg/L and girdling recorded the highest significant yield values (17.78 ± 0.29 and 18.26 ± 0.23 kg/vine) in both seasons, respectively, in contrast to ethephon and girdling treatments which had no significant influence. Besides, BL 2.0 mg/L + girdling presented high levels of physicochemical characteristics of clusters and berries compared to the control. Concerning berry color assessment, ethepon followed by BL at 2.0 mg/L + girdling recorded the highest significant improvement in the red color, as indicated by Color Index of Red Grapes and visual assessment, and had similar and elevated levels of anthocyanin content. On the other hand, flavonoids (3.46 ± 0.03 and 3.66 ± 0.02 mg/100 g FW), and antioxidant capacity (2.37 ± 0.02 and 2.43 ± 0.06 IC 50 ) were obviously increased with BL 2.0 mg/L + girdling, with a slight decrease than ethephon application. As for phenylalanine ammonia-lyase activity, the data show that it increased by ethephon (7.91 ± 0.02 and 8.01 ± 0.02 U mg −1 protein) and BL 2.0 mg/L + girdling (7.77 ± 0.04 and 7.79 ± 0.07 U mg −1 protein), compared to the control (3.69 ± 0.02 and 3.80 ± 0.01 U mg −1 protein) in both seasons, respectively. In terms of polyphenol oxidase activity, the increase in PPO was closely associated with PAL. Moreover, BL 2.0 mg/L + girdling was the most effective treatment for increasing dormant season parameters in both seasons, while ethephon spraying had no influence. All these confirmed that the combined treatment BL 2.0 mg/L + girdling exhibited a synergistic effect in improving the color and quality of 'Crimson Seedless'.
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