Reverse Transcription-PCR Cloning of Macrophage Inflammatory Protein 1α Receptor and C-X-C Chemokine Receptor

Oh, Kwi-Ok,Kwak, Yak-Hoon,Jeong, Seung-Ryong,Lee, Dong-Whan,So, Seo-Young,Song, Yo-Han,Kim, Kack-Kyun The Official Publication of Korean Academy of Oral 1995 International Journal of Oral Biology Vol.19 No.2

The chemokines are a superfamily of 8-10 KDa soluble proteins that have been implicated in chemotaxis and a wide range of inflammatory and immune reactions. They are classified into two branches C-C and C-X-C superfamily according to their primary structure. MIP-1α is a member of C-C chemokine superfamily. For the cloning of murine(m) MIP-1α receptor(R), we used synthetic oligonucleotides corresponding to transmembrane(TM) conserved sequences of already cloned human(h)IL-8-R as primers, and performed RT-PCR amplification using murine macrophage cell mRNA. Among 5 RT-PCR products, we isolated a homologous cDNA to hIL-8-R that presumably is a putative mIL-8-R cDNA. While this study was being undertaken, cloning of hMIP-1α-R has been done in other laboratory. We, therefore, synthesized oligonucleotides corresponding to hMIP-1α-R TM sequences and performed RT-PCR cloning of mMIP-1α-R from murine cytolytic T lymphocyte mRNA, using the oligonucleotides as primers. One putative mMIP-1α-R cDNA was isolated. cDNA librany of CTLL-R8 cell was screened with this cDNA as a probe, and one positive clone was identified.

Expression of Recombinant Macrophage Inflammatory Proteins

Oh, Kwi-Ok,Kim, Kack-Kyun 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2

T 임파구로부터 추출한 cDNA library에서 얻은 몇 가지 미확인 분자 중 L2G25B와 L2G25C는 그 추측 아미노산 배열이 대식세포 염증 단백질 MIP-1α 및 -B(macrophage inflammatory protein-1α, -1β)와 같음이 알려졌다. MIP-1α^1와 -1β는 분자량이 약 5kDa이며 아미노산 배열이 67%까지 일치하고, 골수내에서 조혈을 조절하는 효과를 보이는 것으로 밝혀졌다. 특히 MIP-1α는 초기 전구세포(progenitor cell)에 대하여 억제 효과를 보이며 MIP-1β는 그러한 MIP-1α의 작용을 상쇄하는 것으로 알려져 그 기전을 밝히는 일은 매우 의미있는 일로 생각된다. 따라서 저자는 L2G25B 및 C를 각각 bovine papilloma viral vector 및 baculoviral vector system을 이용, C127 조섬유세포 및 sf21 곤충세포에서 다량 발현, 순수분리하였다.

Prevention of Biofilm Infections

Oh, Kwi-Ok,Kim, Kack-Kyun 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

Biofilms are well-organized, complex microbial communities that are often highly resistant to antimicrobial agents and host defenses. Biofilms are often formed on the surfaces of surgical implants and indwelling catheters. Being extremely resistant to removal, biofilms, once formed, cause numerous complications and often result in persistent infections that require long-term hospitalization for treatment. Until now, preventive measures employing prophylactic antimicrobials that prohibit or restrict biofilm formation have been the only feasible, effective options available, with the constant concomitant threat of antimicrobial resistance. However, the development of chemical agents that specifically act upon the virulence of biofilms, rather than destroying the microorganisms or suppressing their growth, is a promising new approach. Such agents are highly desirable in that they might allow clinicians to prevent the development of antimicrobial resistance. Effective suppression of biofilm formation would dramatically change the way to treat infectious disease. In this literature review, the types of infections associated with biofilms and relevant therapeutic options that have been approved, in use, or under development to treat biofilm infections are discussed, along with novel approaches to biofilm control that may be applicable to the development of future anti-biofilm agents.

Prevention of Biofilm Infections

Kwi-Ok Oh,Kack-Kyun Kim 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

Gene induction by glycyrol to apoptosis through endonuclease G in tumor cells and prediction of oncogene function by microarray analysis

Lee, SungGa,Oh, Hyun-Mee,Lim, Won-Bong,Choi, Eun-Ju,Park, Young-Na,Kim, Jeong-Ah,Choi, Ji-Young,Hong, Suk-Jin,Oh, Hee-Kyun,Son, Jong-Keun,Lee, Seung-Ho,Kim, Ok-joon,Choi, Hong-ran,Jun, Chang-Duk Lippincott Williams Wilkins, Inc. 2008 ANTICANCER DRUGS Vol.19 No.5

Glycyrrhiza uralensis (Leguminosae) has long been known as an antiinflammatory agent for gastric ulcers, arthritis, and rheumatism. The flavonoid glycyrol (GC) (10 μg/ml) isolated from G. uralensis dramatically inhibits phorbol ester (phorbol 12-myristate 13-acetate)-induced nuclear factor (NF)-&kgr;B-dependent transcriptional activity, as determined by luciferase reporter activity in human kidney epithelial 293T cells. To investigate global gene expression profiling in cells by GC, we performed high-density oligonucleotide microarrays. Our microarray analyses showed that GC inhibited phorbol ester-induced NF-&kgr;B-dependent transcriptional activity in inflammatory-related gene expression. RT-PCR analysis, based on microarray data, showed that NF-&kgr;B-dependent genes (such as CCL2, CCL7, CD44, and HSPB8 in addition to NF-&kgr;B itself) were significantly downregulated by GC. Treatment with GC (10 μg/ml) inhibited I-&kgr;B degradation induced by phorbol 12-myristate 13-acetate. The microarray data also suggested that GC induces gene expression to p53-dependent apoptosis through endonuclease G, instead of CAD/DFF and AIF/PDCD8, as a downstream-apoptosis factor in human kidney epithelial 293T tumor cells, and induces oncogenes with a suppressor role as an added function.

인공치면세균막 제거 방법에 의한 RBM 처리 임플란트의 표면거칠기 변화

오희균 ( Hee Kyun Oh ),하명옥 ( Myung Ok Ha ),정성숙 ( Seong Soog Jeong ),정은주 ( Eun Ju Jung ),최충호 ( Choong Ho Choi ),홍석진 ( Suk Jin Hong ) 대한예방치과·구강보건학회 2009 大韓口腔保健學會誌 Vol.33 No.2

Objective: The objective of this study was to evaluate the change in the surface roughness that is being produced by physical or chemical materials for removing of dental plaque from the surface of an RBM(resorbable blasting media)- treated implant. Methods: The RBM treated implant discs were used after artificial plaque was formed by Streptococcus mutans. This study is composed of one control, three physical and three chemical methods that were used to remove dental plaque. The physical methods applied on the RBM implant were prophyflex, professional mechanical tooth cleaning and interdental brushing. The chemicals methods applied to remove plaque on the RBM implants were 0.1% chlorhexidine, citric acid and HCL tetracycline. Each disc was sonically cleaned, air dried, and measured by a profilemeter and a scanning electronic microscope (SEM). Results: There were no statistically significant differences between the control group (1.671±0.11) and each of the other groups (physical: 1.614±0.12, chemical: 1.624±0.09) in the surface roughness values (Ra:μm) after the methods of oral hygiene treatment (p>0.05) have been applied. SEM analysis showed that after each of the oral hygiene treatment methods, all of the groups looked like the original RBM implant surface as hydroxyapatite particles with irregular shapes and sizes were adhered to the surface of the RBM implants. Conclusion: The RBM implant surface was not affected by the six cleaning methods used in this study. These methods are all able to safely remove the plaque or calculus on RBM implants.

마이크로파용 고효율 Doherty 전력증폭기 설계

오정균(Jeong-Kyun Oh),김동옥(Dong-Ok Kim) 한국항해항만학회 2006 한국항해항만학회지 Vol.30 No.5

본 논문에서는 마이크로파 대역의 주파수를 이용해 고효율 도허티 전력 증폭기를 설계 및 제작하였다. 도허티 전력증폭기는 MRF 281 LDMOS FET를 사용하여 구현하였고,도허티 전력 증폭기의 성능을 AB급 증폭기만 있을 때와 비교하였다. 측정결과, 구현한 도허티 전력 증폭기는 P1dB 출력전력이 2.3GHz 주파수에서 33.0dBm을 가진다. 또한,도허티 증폭기는 주파수 2.3GHz~2.4GHz에서 이득은 lldB, 입력반사손실 17.8dB를 얻었다. 설계된 도허티 증폭기는 AB급 증폭기만 있을 때와 비교해서 평균 PAE는 10% 이상 개선됨을 보였고,설계된 도허티 증폭기의 최대 PAE는 39%를 갖는다. In this paper, the high efficiency Doherty power amplifier has been designed and realized for microwave applications. The Doherty amplifier has been implemented using silicon MRF 281 LDMOS FET. The RF performances of the Doherty power amplifier (a combination of a class AB carrier amplifier and a bias-tuned class C peaking amplifier) have been compared with those of a class AB amplifier alone. The realized Doherty power amplifier P1dB output power has 33dBm at 2.3GHz frequency. Also the Doherty power amplifier shows lldB gain and -17.8dB input return loss at 2.3GHz to 2.4GHz. The designed Doherty amplifier has been improved the average PAE by 10% higher efficiency than a class AB amplifier alone. The Maximum PAE of designed Doherty power amplifier has been 39%.

New Isotype of the Peroxiredoxin in Chinese Cabbage and Its Property Comparison to 2Cys-Peroxiredoxin Isolated from the Same Plant

Choi, Yeon-Ok,Cheong, Na-Eun,Lee, Kyun-Oh,Jung, Bae-Gyo,Jeong, Jin-Hee,Chi, Young-Hoon,Cho, Moo-Je,Lee, Sang-Yeol Gyeongsang National University Chinju, Korea 1998 Plant molecular biology and biotechnology research Vol.1998 No.

Effects of Organic Acids on In Vitro Ruminal Fermentation Characteristics and Methane Emission

Ji Un Ok(옥지운),Dong Uk Ha(하동욱),Shin Ja Lee(이신자),Eun Tae Kim(김언태),Sang Suk Lee(이상석),Young Kyun Oh(오영균),Kyoung Hoon Kim(김경훈),Sung Sill Lee(이성실) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

본 연구의 목적은 organic acids를 첨가하여 in vitro 상의 반추위 발효성상과 반추위 내 메탄 억제에 미치는 영향에 대한 효과를 알아보고자 하였다. 반추위액은 순천대학교 부속목장의 반추위 cannula가 시술된 Holstein에서 채취하였고, organic acids는 반추위액과 버퍼의 혼합액에 첨가하여 배양하였다. 그 결과 pH 값은 lactic acid, malic acid 및 succinic acid첨가구에서 6.69에서 6.16 정도로, 대조구와 다른 첨가구보다 낮았다. 총 가스 발생량은 배양 48시간에 aspartic acid, malic acid 및 succinic acid첨가구에서 유의적(p<0.05)으로 높았고, 메탄 발생량은 lactic acid 첨가구에서 대조구보다 낮았다. 총 VFA와 propionic acid의 농도는 배양 12시간에 모든 첨가구가 대조구에 비해 높았다. 반추위 미생물 측정 결과에서는 Fumaric acid와 malic acid의 bacteria수가 대조구에 비해 유의적으로 증가하였으며(p<0.05), protozoa수는 유의적(p<0.05)으로 감소되었다. 이상의 실험 결과를 종합해 보면, organic acids의 첨가는 반추위 내 pH를 감소시키고 가스 발생량, 반추위 미생물 성장량 및 propionic acid 모두 증가시켰으며, 특히 lactic acid는 메탄생성을 억제하였다. 앞으로 Organic acid와 다른 메탄억제 물질과 혼합하여 반추위 내 메탄생성 억제에 관한 구체적인 연구가 필요한 것으로 사료된다. The objective of this study was to evaluate the in vitro effects of organic acids on methane emission and ruminal fermentation characteristics. We expected our methodology to result in a decrease of methanogens attached to the surface of rumen ciliate protozoa by addition of organic acids and in particular a decrease in methane emission. A fistulated Holstein cow of 650 kg body weight was used as a donor of rumen fluid. Organic acids (aspartic acid, fumaric acid, lactic acid, malic acid, and succinic acid) known to be propionate enhancers were added to an in vitro fermentation system and incubated with rumen fluid. The microbial population, including bacteria, protozoa, and fungi, were enumerated, and gas production, including methane and fermentation characteristics, were observed in vitro. Organic acids appeared to affect the rumen protozoan community. The rumen protozoal popuation decreased with the addition of aspartic acid, fumaric acid, lactic acid, and malic acid. In particular, the methane emission was reduced by addition of lactic acid. The concentration of propionate with all organic acids that were added appeared to be higher than that of the control at 12 h incubation. Addition of organic acids significantly affected rumen bacteria and microbial growth. The bacteria in added fumaric acid and malic acid was significantly higher (p<0.05) and protozoa was significantly lower (p<0.05) than that of the control. Microbial growth with the addition of organic acids was greater than the control after 48 h incubation.

만성 B 형 바이러스성 간염환자에서 lamivudine 52 주 투여의 치료효과에 대한 연구

견진옥(Jin Ok Kyun),오정미(Jung Mi Oh),조성원(Sung Won Cho) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.2

Lamivudine, an oral nucleoside analogue, effectively inhibits hepatitis B virus replication and reduces hepatic necroinflammation in patients with chronic hepatitis B. Although lamivudine has shown a promise in patients with chronic hepatitis B, a long-term data on Korean patients with hepatitis B are lacking. The purpose of this study is to evaluate the effects and safety of 52-week lamivudine therapy in Korean patients with chronic hepatitis B. A total of twenty-nine patients (27 male and 2 female) who had received 100 mg of oral lamivudine daily for 52 weeks were evaluated, retrospectively. The mean age of 29 patients in the study group was 37.7±8.9 years (range 19-54). Pretreatment HBV PCR and HBsAg were positive in all 29 patients, and HBeAg were positive in 25 patients (86%). The serum HBV DNA of 28 patients (97%) significantly fell to undetectable levels (<5 pg/ml) within 12 weeks of therapy and it remained undetectable in 24 patients (83%) by the end of 52-week therapy (p<0.001). Mean serum ALT levels of 29 patients declined to the normal range within 12 weeks and remained within the normal range during the evaluative period (p<0.05). The proportions of patients with HBeAg seroconversion (loss of HBeAg, development of antibody to HBeAg, and undetectable HBV DNA) were 42% after 52-week therapy. The differences of response to lamivudine therapy in HBeAg-positive and HBeAg-negative patients were negligible (p>0.05). Furthermore, the study showed that pretreatment serum HBV DNA and ALT levels have no effect to the efficacy of lamivudine therapy (p>0.05). Further comparison of lamivudine`s efficacy showed that lamivudine is just as efficacious in patients with cirrhosis as without cirrhosis (p>0.05). In conclusion, lamivudine is an effective and safe therapy for the treatment of chronic hepatitis B in Korean patients.