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Ohn Mar Lynn,김장억,이경열 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.1
The effects of azadirachtin on the rates of pupation, adult eclosion, and the expression of nine selected genes were evaluated in Plodia interpunctella. The newly molted fifth instar larvae (V0) were fed a pollen diet containing different amounts of azadirachtin. When larvae ingested azadirachtin at 1 and 10 ppm, pupation was 85 and 75.8%, respectively, and adult eclosion was 81.7 and 55.3%, respectively. The effects of azadirachtin on gene expression were analyzed using real-time RT-PCR analysis. When V0 larvae ingested azadirachtin for 2 days, expression of some genes related to development, stress, and immunity was affected by azadirachtin. mRNA levels of shsp, hsp90, ecdysone receptor, ultraspiracle, prophenoloxidase and hemolin were upregulated,although both ultraspiracle and hemolin were downregulated at higher doses. Levels of hsc70, hsp70 and β-1,3-glucan recognition protein were not changed. Overall, azadirachtin significantly inhibited post-embryonic development and differentially modulated gene expression patterns of P. interpunctella.
Lynn, Ohn Mar,Song, Woo-Geun,Shim, Jae-Kyoung,Kim, Jang-Eok,Lee, Kyeong-Yeoll The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5
Toxicity of the plant-derived natural pesticide azadirachtin and two types of commercial neem (Azadirachta indica A. Juss)-based formulations, Neema (liquid type) and Neema-plus (pellet type), were evaluated based on the mortality rate and developmental inhibition of the sweetpotato whitefly (Bemisia tabaci) and root-knot nematode (Meloidogyne incognita). In a laboratory assay, when B. tabaci adults were fed leaves containing 5 or 10 ppm of azadirachtin solutions, the rates of female oviposition, subsequent egg hatch, and adult eclosion were significantly reduced to 23.1, 53.2, and 26.6% of the control, respectively. At a tomato greenhouse, the rates of adult colonization, oviposition and egg hatch were reduced to 78.2, 47.0, and 71.2% by Neema foliar spray and 31.3, 34.1, and 66.8% by soil treatment with Neema-plus relative to the control, respectively. When isolated soil nematodes were exposed to various concentrations of azadirachtin, Neema, and Neema-plus, the immobility of juvenile nematodes showed no change at 2 h after treatment, whereas a reduction of 36.3% was observed at day 1 with 10 ppm of azadirachtin. Nevertheless, the effects of neem formulations were faster and much higher than those of azadirachtin. At a cucumber greenhouse, soil treatments with neem formulations significantly reduced the numbers of soil nematodes and plant root-knots; the reduction with Neema was 12.1 and 9.0%, and with Neema-plus 26.4 and 24.6% of the control, respectively. Furthermore, soil treatment with Neemaplus greatly improved the growth of cucumber plants in nematode-infested pots. These results showed that azadirachtin and neem-based formulations were highly effective on the developmental inhibition of both whiteflies and root-knot nematodes. Thus, soil application of the neem-based formulations would be applicable for the control of both leaf-sucking and soil pests.
Ohn Mar Lynn,Woo Geun Song,Jae Kyoung Shim,Jang Eok Kim,Kyeong Yeoll Lee 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5
Toxicity of the plant-derived natural pesticide azadirachtin and two types of commercial neem (Azadirachta indica A. Juss)-based formulations, Neema (liquid type) and Neema-plus (pellet type), were evaluated based on the mortality rate and developmental in
Incidence of virus diseases and its vectors in Myanmar
Nang Kyu Kyu Win,Ohn Mar Lynn,Tin Tin Aye 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Various symptoms of virus diseases are observed on the cultivated and non-cultivated crops in Myanmar. The virus diseases cause a major limiting factor in the tomato and chili production. During 2018, survey on the virus disease and its vectors in tomato and chili fields were conducted in central regions of Myanmar including Naypyidaw, Mandalay, Magway and Sagaing Divisions. The symptoms observed on tomato and chili were chlorosis, puckering of leaf, upward curling of leaves, reduction in leaf size, vein and stunting. Tomato leaf curl (TLC) disease incidence and severity ranged from 10 to 100% and 37 to 86% respectively. Chili leaf curl (CLC) disease incidence and severity was 30 to 100% and 13 to 100% respectively. The highest incidence and severity of TLC and CLC recorded in Lewe townships, Naypyidaw. Insect vectors observed in those areas were whitefly (Bamisia tabaci), jassid (Emposca sp) and mealy bug, and the white fly was common. Whiteflies are very important vectors for virus in tropical and subtropical regions, but not confirmed to this area. Jassids are also vectors for virus. Both nymphs and adults suck the plant sap and introduce toxin to host plants. Therefore, an infestation may be very damaging and show stunted growth and bushy appearance.