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RISS 인기검색어
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- 저자 : Odair Alberton (검색결과 2 건)
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Meire Pereira de Souza Ferrari,Rayane Monique Sete da Cruz,Mayara dos Santos Queiroz,Matheus Marquezine de Andrade,Odair Alberton,Hélida Mara Magalhães 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.5
Acclimatization is the most delicate stage of micropropagation, which makes it challenging to obtain new seedlings. The use of suitable substrates associated with arbuscular mycorrhizal fungi (AMFs) has been promising during this stage. The goal of this study was to investigate the infl uence of diff erent AMFs and substrates on the acclimatization of Curcuma longa L. The C. longa seedlings were obtained from in vitro cultivation with a specifi c medium for 120 days. After this period, they were transplanted into plastic pots containing two substrate formulations. The fi rst compound was soil and vermiculite (1:1 v/v), and the second was a commercial substrate for vegetables, containing vermicompost and vermiculite (1:1:1 v/v). The fungi used in the study were Rhizophagus clarus, Claroideoglomus etunicatum, and a mixture of the two fungi. A treatment with no fungi was used as the control. After 240 days, the growth and structural characteristics of the plant were measured, as well as nutrients and soil–quality parameters. The use of a substrate rich in organic matter (substrate 2) associated with R. clarus and C. etunicatum promoted 100% survival of C. longa plants. In this condition, the plants were more vigorous. The root system increased in size and biomass, being robust with the presence of many roots. The soil indicators revealed that the fungi helped with the lower activity of the metabolic quotient and increased basal respiration, indicators that demonstrated the benefi cial action of the fungi for the plants. In addition, changes in stomatal conformation and density probably allowed greater CO 2 diff usion.
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Genotoxicity Study of Vitex megapotamica (Spreng.) Moldenke
Rita de Cássia Lima Ribeiro,Emerson Luiz Lorenço Botelho,Guilherme Donadel,Maria Leticia Ames,Bruna Nunes,Salviano Tramontini,Andreia Assunção Soares,Odair Alberton,Ezilda Jacomassi,Arquimedes Gasparo 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.7
Vitex megapotamica (Spreng) Moldenke is commonly known as tarumã, it is an important medicinal and edible fruit plant. It is native to regions of tropical and subtropical climate in greater proportion than temperate zones and widely distributed in Central America, South America, Asia, and Africa. In Brazil, it is present in the Atlantic Forest and Cerrado biomes. Despite its widespread use, there are no minimum standards for quality control or information on genotoxicity. Therefore, the aim of this study was to present a detailed description of the short-term genotoxicity assays of V. megapotamica and to provide parameters of a preparation routinely used in traditional folk medicine. For genotoxicity assays, five groups were used with eight wistar rats in each group. For this, three doses of the V. megapotamica extract in doses (100, 300, and 900 mg/kg) or negative control (filtered water) were administered orally and positive control cyclophosphamide monohydrate (20 mg/kg; Sigma–Aldrich®) was applied by the intraperitoneal route after 24 h. At the end, whole blood was collected in a tube containing EDTA for the comet test and later the animals were euthanized. For the micronucleus test, femurs were removed, and bone marrow was collected. In the comet assay, V. megapotamica crude extract did not show significant DNA damage at all doses tested. The micronucleus assay showed no significant increase in the frequency of inducing micronuclei at any dose examined. It can be concluded that the safety parameters in genotoxicity studies reveal that V. megapotamica has no toxicity, which characterizes the important quality control of this plant species.
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