Efficacy of a high quality O₁/Campos foot-and-mouth disease vaccine upon challenge with a heterologous Korean O Mya98 lineage virus in pigs

Galdo Novo, S.,Malirat, V.,Maradei, E.D.,Pedemonte, A.R.,Espinoza, A.M.,Smitsaart, E.,Lee, K.N.,Park, J.H.,Bergmann, I.E. Elsevier 2018 Vaccine Vol.36 No.12

<P><B>Abstract</B></P> <P>In 2010 serotype O foot-and-mouth disease virus of the Mya98 lineage/SEA topotype spread into most East Asian countries. During 2010–2011 it was responsible for major outbreaks in the Republic of Korea where a monovalent O/Manisa vaccine (belonging to the ME-SA topotype) was applied to help control the outbreaks. Subsequently, all susceptible animals were vaccinated every 6 months with a vaccine containing the O/Manisa antigen. Despite vaccination, the disease re-occurred in 2014 and afterwards almost annually. This study focuses on the <I>in vivo</I> efficacy in pigs of a high quality monovalent commercial O<SUB>1</SUB>/Campos vaccine against heterologous challenge with a representative 2015 isolate from the Jincheon Province of the Republic of Korea. Initially, viral characterizations and r<SUB>1</SUB> determinations were performed on six viruses recovered in that region during 2014–2015, centering on their relationship with the well characterized and widely available O<SUB>1</SUB>/Campos vaccine strain. Genetic and antigenic analysis indicated a close similarity among 2014–2015 Korean isolates and with the previous 2010 virus, with distinct differences with the O<SUB>1</SUB>/Campos strain. Virus neutralisation tests using O<SUB>1</SUB>/Campos cattle and pig post vaccination sera and recent Korean outbreak viruses predicted acceptable cross-protection after a single vaccination, as indicated by r<SUB>1</SUB> values, and in pigs, by expectancy of protection. In agreement with the <I>in vitro</I> estimates, <I>in vivo</I> challenge with a selected field isolate indicated that O<SUB>1</SUB>/Campos primo vaccinated pigs were protected, resulting in a PD50 value of nearly 10. The results indicated that good quality oil vaccines containing the O<SUB>1</SUB>/Campos strain can successfully be used against isolates belonging to the O Mya98/SEA topotype.</P>

Poster Session:PS 0206 ; Family Medicine : Comprehensive Initial Training for Medical Doctors Starting in 1980 in Musashino Red Cross Hospital

( Marta Perez De Lis Novo ),( Roberto Perez Lyarez ),( Pilar Brito Zeron ),( Antoni Siso Almirall ),( Belchin Kostov ),( Mireia Marti Villalta ),( Albert Bove ),( Hoda Gheitasi ),( Soledad Retamozo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Medical education for medical school graduates in Japan greatly changed after World War II. From 1948, the General Headquarters (GHQ), the Supreme Commander for the Allied Powers imposed internship medical school graduates as early clinical training, internal medicine for 5 months, surgery for a month, obstetrics and gynecology for a month, public health for a month, for initial year. This system was unpaid before the nation examination, and abolished in 1968 by the campus dispute spread throughout Japan. Subsequently became mainstream medical center by the universities and colleges, but did not develop the training of general practitioners and family doctors in Japan because of aiming for the priority of specialization by university faculty of medicine. Musashino Red Cross Hospital (MRCH) started comprehensive initial training for medical doctors since 1980. One of the reason is that The Japan Red Cross Society as the most important philosophy advocates disaster medicine and its realization in general medical education is very important. Methods: MRCH employed the intern doctors by general invitation examination, and educated them for two years. In principle mandatory training period within 2 years in internal medicine, surgery, pediatrics, obstetrics and gynecology training, psychiatry, and regional medical training. Results: From 1980 to now MRCH did general medical education to more than 200 doctors. These doctors are working around the world. In Japan, from 2004 Initial general medical training was compulsory to all new medical doctors for two years. Conclusions: Comprehensive initial training for medical doctors is very important in general medicine for all the generations and the disaster medicine. In Japan from the year 2020 general medicine training under the new medical program scheduled.

Slide Session :OS-NEP-05 ; Nephrology : Ormond`s Disease (Retroperitoneal Fibrosis) and Idiopathic Mesenteritis in Internal Medicine: Possible IgG4-Related Diseases?

( Marta Perez De Lis Novo ),( Roberto Perez Alvarez ),( Soledad Retamozo ),( Pilar Brito Zeron ),( Xavier Bosch ),( Antoni Siso Almirall ),( Jaume Villalta ),( Ricardo Alvarez Vijande ),( Alfonso Lope 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To analyse the possible existence of features associated with IgG4-related disease (IgG4-RD) in patients diagnosed with retroperitoneal fi brosis or mesenteritis. Methods: Study cohort including patients diagnosed with retroperitoneal fi brosis and/ or mesenteritis by imaging studies (CT) in a university hospital during the last 20 years. Results: A total of 22 patients diagnosed with retroperitoneal fi brosis (15 patients) or mesenteritis (7 patients) are described (6 women and 16 men, with a mean age at diagnosis of 64.1 years, range: 46-82. The main clinical manifestations present at diagnosis were abdominal/back pain (n=14), general malaise/fever (n=7) and the presence of edema/thrombosis (n = 6); in one patient, diagnosis was made incidentally by imaging techniques. CT showed infl ammatory masses affecting retroperitoneal (n=17), mesenteric (n=7) and vascular structures, including iliac arteries (n=3), aorta (n=2), renal arteries (n=2) and cava (n=2). In 7 (32%) patients, involvement of other organs suggestive of IgG4-RD (liver/bile tract in 2 cases, periaortitis in 2 cases, pleura, lymph nodes and skin in 1 each, respectively) was found. Diagnosis was confi rmed by biopsy in 8 patients (36%); in any patient, studies to rule out IgG4-RD were performed. The main treatments included corticosteroids (n=17), immunosuppressive agents (azathioprine in 5, methotrexate in 1 and mycophenolate in 1), surgery (n = 5) and the placement of urinary catheters (n = 4). After a mean follow-up of 73 months, 7 patients died (32%) patients (4 of them due to septic shock). Conclusions: Ormond`s disease is a rare clinical entity but associated with a signifi cant morbidity and mortality. His recent inclusion in the clinical spectrum of IgG4-RD diffi cult their retrospective diagnosis, although this study and others recently published suggest that about half of the cases could correspond to IgG4-RD.

Poster Session : PS 0388 ; Infectious Disease ; Infectious Spondylodiscitis: The Importance of Clinical Suspicion

( Marta Perez De Lis Novo ),( Alexandre Perez Gonzalez ),( Melania Estevez Gil ),( Emma Filgueira Davila ),( Julian Fernandez Martin ),( Roberto Perez Alvarez ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To analyse the epidemiological, clinical, diagnostic and therapeutic features related to the development of spondylodiscitis in a cohort of Spanish patients. Methods: A retrospective analysis of 75 cases of infectious spondylodiscitis diagnosed in university hospital between 2000 and 2013. Results: We studied 75 patients with a mean age of 63 + / - 20 years, of which 67% were male. Time average evolution of disease prior to consultation was 4.2 months. The main symptom was back pain or back pain (90%) The most frequent location was the lumbar spondylodiscitis (74%), 60% of patients had one or more associated diseases (Diabetes:20%; COPD: 18%; tumors: 17%). Furthermore, 50% of them suffered from heart disease, being the most frequently hypertensive. 23% of patients carried immunosuppressive therapy, mainly corticosteroids (90%). In 53 patients (70%) could be performed a certain diagnosis, with Staphylococcus aureus the most frequent (41%) followed by S. epidermidis (17%), and Streptococcus (15%). Blood cultures were positive in 60% of cases and surgical biopsy was performed in 37% of the patients. 8 cases had endocarditis criteria, 6 of which were fulfi lled by S. aureus. MRI was performed in 75% cases, being in all cases, pathological. The average length of treatment was 12 weeks, with the most commonly used antibiotics cloxacillin, vancomycin and ceftriaxone. 18% of patients underwent surgery, performed laminectomy and drainage of abscesses. During evolution 6 patients died. 10 patients (8%) had sequelae neurological. Conclusions: Spondylodiscitis is a rare disease in adults, but with high morbidity and mortality due to the diffi culty of diagnosis. Insidious development and the lack of specifi city of the symptoms it interferes with the diagnosis and treatment early, so a high index of suspicion is essential.

Poster Session:PS 0207 ; Family Medicine : The Cardiovascular Paradox of Sle in Family Medicine: A Case-Control Study of the Prevalence and Management of Cardiovascular Disease in Patients with Sle Followed in a Primary Care Center

( Marta Perez De Lis Novo ),( Roberto Perez Lvarez ),( Pilar Brito Zeron ),( Antoni Siso Almirall ),( Belchin Kostov ),( Mireia Marti Villalta ),( Albert Bove ),( Hoda Gheitasi ),( Soledad Retamozo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To analyze the prevalence of SLE in the field of primary care and to evaluate the management of cardiovascular risk factors (CVRF) in comparison with a non-autoimmune control population. Methods: Analysis of the diagnostic accuracy of SLE by primary care physicians using medical record audit (EMR) in 3 health centers including a population of 44,184 inhabitants. The prevalence of CVRF and clinical cardiovascular disease (CVD) in the SLE population was evaluated and compared with a control population of patients without autoimmune disease matched for gender. Results: 145 patients were identifi ed as SLE. After a case-by-case audit, 112 (77%) patients were confirmed as having SLE, representing a prevalence of 0.25%: 92% were women, with a median follow-up of 11.3 years: death was recorded in 8.9%. The comparison of the main characteristics between SLE and the control group showed that SLE patients had a lower mean age (53.2 vs 60.5, p=0.001), a lower frequency of dyslipidemia (26% vs 47%, p=0.001), a lower mean total cholesterol (199.5 vs 211.3 mg/dL, p=0.023) and a lower mean systolic blood pressure (119 vs. 124.2 mmHg, p=0.043). In contrast, patients with SLE had a higher frequency of renal disease (32.1% vs 12.1, p<0.001), cerebrovascular disease (8.0% vs 1.6%, p=0.027) and non-fatal cardiovascular events (17.0% vs 4.8%, p=0.003). Conclusions: The level of diagnostic accuracy of SLE in our primary care area was high. We found a ‘cardiovascular paradox “: despite having a signifi cantly better degree of control of the main CVRF, and even being a younger population, a higher prevalence of CVD was observed in SLE patients, suggesting that this autoimmune disease is, per se, a cardiovascular risk factor.

The Paleoparasitology in Brazil and Findings in Human Remains from South America

Shenia Patricia Correa Novo,Luiz Fernando Ferreira 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.5

Current Knowledge of Small Flukes (Digenea: Heterophyidae) from South America

Cláudia Portes Santos,Juliana Novo Borges 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.4

Fish-borne heterophyid trematodes are known to have a zoonotic potential, since at least 30 species are able to infect humans worldwide, with a global infection of around 7 million people. In this paper, a ‘state-of-the-art’ review of the South American heterophyid species is provided, including classical and molecular taxonomy, parasite ecology, host-parasite interaction studies and a list of species and their hosts. There is still a lack of information on human infections in South America with undetected or unreported infections probably due to the information shortage and little attention by physicians to these small intestinal flukes. Molecular tools for specific diagnoses of South American heterophyid species are still to be defined. Additional new sequences of Pygidiopsis macrostomum, Ascocotyle pindoramensis and Ascocotyle longa from Brazil are also provided.

Endogenous Preferential Trade Agreements

( Sanchez De Paz Elena ),( Novo Peteiro Jose Antonio ),( Mendez Naya Jose ) 세종대학교 경제통합연구소 1999 Journal of Economic Integration Vol.14 No.3

This paper shows that preferential trade agreements can emerge in an endogenous way. We use a simple international trade model where governments fix their tariffs in order to maximize social welfare. We find that when countries behave in a non co-operative way this performance leads to tariff discrimination. This result holds whether firms play a Cournot strategy or whether they follow a Stackelberg`s leader-follower strategy. This paper also analyzes whether multilateralism and regionalism are complementary or substitutive processes. It is concluded that, in spite of the fact that absolute protection is reduced as a result of the economic integration process, relative protection against the rest of the world increases and, therefore, the two processes should not be considered as complementary. (JEL Classifications: F15; F14; F13.)

Slide Session : OS-CAD-03 ; Cardiology : Spanish Registry of Autoimmune Congenital Heart Block in Babies of Mothers Carrying Anti-Ro/la Antibodies (Rebacc-Geas-Semi)

( Pilar Brito Zeron ),( Marta Perez De Lis Novo ),( Pilar Rosich ),( Carles Tolosa ),( Joaquim Oristrell ),( Luis Saez Comet ),( Mercedes Perez Conesa ),( Jose Antonio Vargas Hitos ),( Jose Mario Sabi 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To analyze the outcomes and therapeutic management of affected pregnancies with autoimmune congenital heart block (aCHB). Methods: Retrospective multicenter study in Internal Medicine Departments; inclusion criteria consisted of: aCHB of any type (I, II or III), fetal EFE and/or cardiomyopathy, cardiac block diagnosed in utero or in the first postpartum month, and mothers carrying anti-Ro52, Ro60 and/or La autoantibodies. Results: A total of 25 pregnancies with aCHB were retrospectively analyzed in 21 anti-Ro/La+ mothers. The mean maternal age at the time of pregnancy with aCHB was 33,25 years. Only 2 mothers received treatment prior to the first affected pregnancy (hydroxychloroquine and hydroxychloroquine+prednisone). Cardiac block consisted of type I (n=1), type II (n=6) and type III (n=18). At diagnosis of aCHB, 15/22 women were treated with dexamethasone (one of them, along with IVIG) and 2/22 with ritodrine. Preventive treatment with IVIG was administered in 2 pregnancies in which a recurrence was observed. Pregnancy was interrupted in 7/25 pregnancies at a mean week of 23.43 (18.2-37), while 18/25 of pregnancies gave a live birth baby with a mean age of 35.71 weeks of birth (30-40). 11 babies required pacemaker implantation, 9 immediately after birth and 2 in the neonatal period (1 at 5 years of age and another at 12). Of the 15 pregnancies with aCHB treated with dexamethasone, 12 achieved pregnancy to term (1 type II disappeared, with no changes in the remaining cases) and there were 3 fetal deaths despite treatment. Of the 7 pregnancies not treated with dexamethasone, 3 babies were born alive (no reversal of the blockade) and there were 4 fetal deaths. Conclusions: aCHB is a serious problem with a fetal mortality of 28% and a high requirement for neonatal pacemaker placement (61%).

Slide Session : OS-RHEU-08 ; Rheumatology : Minimally-Invasive Biopsy of Minor Salivary Glands in Internal Medicine: Searching for Systemic Infi ltrative Diseases

( Pilar Brito Zeron ),( Marta Perez De Lis Novo ),( Soledad Retamozo ),( Lluisa Alos ),( Albert Bove ),( Hoda Gheitasi ),( Belchin Kostov ),( Antoni Siso Almirall ),( Roberto Perez Alvarez ),( Manuel 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To analyze the safety and utility of minor lip biopsy by using the minimally invasive technique, performed in the Department of internal medicine, in patients presenting with immunonegative sicca syndrome. Methods: Prospective analysis of 122 patients (100 women, mean age 60 years) with sicca syndrome and negative anti-Ro/La antibodies in which minimally invasive biopsy of minor salivary glands was performed. Results: The most common histopathological feature consisted of lymphoplasmacytic infi ltration in 57 (47%) patients, followed by fi brosis in 46 (38%) and acinar atrophy in 32 (26%); the type of lymphoplasmacytic infi ltrate was detailed in 53 patients (21 lymphocytic, 20 plasmacytic, and 12 lymphoplasmacytic). According to the lymphocytic classifi cation of Chisholm and Mason, 53 (44%) were classifi ed as normal, 42 (35%) grade I, 11 (9%) grade II, 8 (7%) grade III and 7 (6 %) grade IV. Histopathologic diagnosis were: normal in 46 patients, non-specifi c sialadenitis in 42, primary Sjogren`s syndrome in 26 (15 as the fi nal diagnosis, 11 and probable), and other diagnoses in 6 (fat infi ltration in 5, systemic amyloidosis in 1); no cases of infi ltration by granulomas or IgG4+ were found. The presence of fi brosis was detected more frequently in women (43% vs 14%, p=0.014); the existence of fi brosis (67.70 vs 55.76 years) and acinar atrophy (69.50 vs 56.71 years) correlated with increasing age. No correlation between the degree of infi ltration and diagnostic or immunological features was found. Only 8% patients reported transient adverse events. Conclusions: In 21% of patients with an immunonegative sicca syndrome, a diagnosis of primary SS was confi rmed by minimally invasive biopsy, whereas 35% of patients disclosed a non-specifi c chronic sialadenitis, which could refl ect a “mild” SS that have no place in the current classifi cation criteria of this disease.