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Growth Response to a GH-Autotransgenesis in Common Carp Cyprinus carpio
Noh, Choong-Hwan,Kim, Dong-Soo The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.1
Autotransgenic manipulation with a growth hormone (GH)-construct is a potential approach to improving the growth rates of farmed fish. Here, we present the generation of GH-autotransgenic common carp Cyprinus carpio carrying a transgene comprised of the carp homologous GH gene and a ${\beta}$-actin regulator. Autotransgenic carp showed similar viability to their non-transgenic siblings. Early growth characteristics of founder autotransgenic carp up to 50 days postfertilization were highly variable among individuals; i.e., some fish exhibited significant growth depression, while others showed dramatic acceleration of growth, achieving greater than sixfold increases in body weight relative to their non-transgenic counterparts. Stimulated growth performance became more notable with age and many transgenic individuals of the largest class reached 5 kg within 8 or 9 months, which is at least 10 times heavier than the average body weight of communally grown non-transgenics. Four of six founder transgenic males were successful in passing the transgene to their $F_1$ offspring with frequencies ranging from 19 to 36%. Growth stimulations were also persistent in all $F_1$ progeny groups examined.
Original Articles : Growth Response to a GH-Autotransgenesis in Common Carp Cyprinus carpio
( Choong Hwan Noh ),( Dong Soo Kim ) 한국수산과학회(구 한국수산학회) 2012 Fisheries and Aquatic Sciences Vol.15 No.1
Autotransgenic manipulation with a growth hormone (GH)-construct is a potential approach to improving the growth rates of farmed fish. Here, we present the generation of GH-autotransgenic common carp Cyprinus carpio carrying a transgene comprised of the carp homologous GH gene and a β-actin regulator. Autotransgenic carp showed similar viability to their non-transgenic siblings. Early growth characteristics of founder autotransgenic carp up to 50 days postfertilization were highly variable among individuals; i.e., some fish exhibited significant growth depression, while others showed dramatic acceleration of growth, achieving greater than sixfold increases in body weight relative to their non-transgenic counterparts. Stimulated growth performance became more notable with age and many transgenic individuals of the largest class reached 5 kg within 8 or 9 months, which is at least 10 times heavier than the average body weight of communally grown non-transgenics. Four of six founder transgenic males were successful in passing the transgene to their F1 offspring with frequencies ranging from 19 to 36%. Growth stimulations were also persistent in all F1 progeny groups examined.
붉바리(Epinephelus akaara)와 Brown-Marbled Grouper (E. fuscoguttatus) 종간 잡종 수정란의 부화율
노충환 ( Choong Hwan Noh ),김근식 ( Keun Sik Kim ),명정구 ( Jung Goo Myoung ),조재권 ( Jae Kwon Cho ),윤락진 ( Nak Jin Yun ),임한규 ( Han Gyu Lim ),방인철 ( In Chul Bang ) 한국어류학회 2015 韓國魚類學會誌 Vol.27 No.1
서식 환경이 다른 바리과(family Serranidae) 어류 두 종, 붉바리 (Epinephelus akaara)와 brown-marbled grouper (E. fuscoguttatus)의 종간 잡종 수정란의 부화율을 조사하였다. 잡종(붉바리 ♀×brown marbled grouper □)의 수정 후 각 발생단계까지 소요시간은 붉바리 순종과 차이가 없었으며,부화 소요시간은 25℃에서 25.6시간이었다. 잡종은 붉바리순종과는 달리 비균등 난할이 분명하게 관찰되었다. 잡종의 부화율은 네 수온(22, 25, 28, 31℃)에서 대체적으로 낮았으며, 25℃에서 9.8%로 가장 높았다. 이상의 연구에서 서식환경이 서로 다른 바리과 두 종간 잡종이 생산 가능한 것으로 조사되었으며, 향후 산업적 활용성을 검증하기 위하여 발생 양상, 기형률 및 초기 생존율 등에 관한 면밀한 연구가 필요할 것으로 판단된다. The hatchability of the artificially induced hybrid between two groupers (family Serranidae), red spotted grouper (Epinephelus akaara) and brown-marbled grouper (E. fuscoguttatus) that lives in different habit environment was investigated. There was no difference in the required time of each developmental stages after fertilization between hybrid (red spotted grouper ♀ ×brown-marbled grouper □) and purebred (red spotted grouper ♀×□) and required 25.6 hours to hatch at incubated in 25℃, but a noticeable unequal cleavage in cell size was observed in hybrid eggs unlikely to purebred. The hatching rate of fertilized eggs of hybrid was generally low across the four incubate temperatures(22, 25, 28, 31℃) with highest 9.8% in 25℃. This study demonstrated the possibility of artificial hybridization between two groupers, red spotted grouper and brown-marbled grouper, thus preparing the groundwork on developmental characteristics, deformities of hatched larvae and early survival ability for further studies on aquaculture.
선발과 비선발 참돔 (Pagrus major) 계통의 성장 양상 비교
노충환 ( Choong Hwan Noh ),홍경표 ( Kyung Pyo Hong ),오승용 ( Sung Yong Oh ),최희정 ( Hee Jung Choi ),박용주 ( Yong Joo Park ),명정구 ( Jung Goo Myoung ),김종만 ( Jong Man Kim ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.5
N/A Growth performances of the selected and the non-selected red sea bream (Pagrus major) lines were evaluated up to marketable size. The offspring were mass-produced either from the selected line (the fourth generation of broods from mass selection) or the non-selected line (the second generation of wild broods). Early (59 to 103 days old, trial I) and late growth performances (4 to 24 months old, trial Ⅱ) were evaluated based on communal rearing in land-based tanks and sea cages, respectively. In trial I, the selected line exhibited significantly higher values in both weight gain (WG) and specific growth rate (SGR) when compared to those observed in the non-selected line (P<0.05). In trial II comprising of three growth phases (4-9, 9-16 and 16-24 months old), selected line showed only slightly improved in WG and SGR at the beginning two growth phases (4-9 and 9-16 months old) (P>0.05), however, displayed significantly higher values at the last growth phase (16-24 months old) (P<0.05). Overall WG and SGR throughout the experiment (4-24 months) were also significantly higher in the selected line than the non-selected line (P<0.05). Consequently, mean body weight of the selected line at 24 months old was 1.4-fold (40.8% increased) as compared to that of the non-selected line. This present result indicates that mass selection of this species against the growth traits might be quite effective, and the present advance has important implication in the productivity enhancement of red sea bream aquaculture.