Screening and Production of Manganese Peroxidase from Fusarium sp. on Residue Materials

( Nguyen Duc Huy ),( Nguyen Thi Thanh Tien ),( Le Thi Huyen ),( Hoang Tan Quang ),( Truong Quy Tung ),( Nguyen Ngoc Luong ),( Seung-moon Park ) 한국균학회 2017 Mycobiology Vol.45 No.1

In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.

Immunogenicity of an S1D epitope from porcine epidemic diarrhea virus and cholera toxin B subunit fusion protein transiently expressed in infiltrated Nicotiana benthamiana leaves

Huy, Nguyen-Xuan,Tien, Nguyen-Quang-Duc,Kim, Mi-Young,Kim, Tae-Geum,Jang, Yong-Suk,Yang, Moon-Sik Springer-Verlag 2016 Plant cell, tissue and organ culture Vol.127 No.2

<P>Porcine epidemic diarrhea virus (PEDV) belongs to the Coronaviridae family and causes acute enteritis in pigs. A fragment of the large spike glycoprotein, termed the S1D epitope (aa 636-789), alone and fused with cholera toxin B subunit, were independently cloned into plant expression vectors, yielding plasmids pMYV717 and pMYV719, respectively. Plant expression vectors were transformed into Agrobacterium tumefaciens and subsequently infiltrated into Nicotiana benthamiana leaves. The highest expression level of S1D was found at 2 days post infiltration (dpi), reached 0.04 % of total soluble protein, and rapidly decreased thereafter. The expression and assembly of CTB-S1D fusion protein were confirmed by Western blot and G(M1)-ELISA. The highest expression level of CTB-S1D fusion protein was 0.07 % of TSP at 4 dpi, with a rapid decrease thereafter. In the presence of p19 protein from tomato bushy stunt virus, the S1D and CTB-S1D protein levels peaked at 6 dpi and were fourfold to sevenfold higher than in the absence of p19, respectively. After oral administration of transiently expressed CTB-S1D fusion protein, or with bacterial cholera toxin or rice callus expressing mutant cholera toxin 61F, mice exhibited significantly greater serum IgG and sIgA levels against bacterial CTB and S1D antigen, peaking at week 6. Transiently expressed CTB-S1D fusion protein will be administered orally to pigs to assess the immune response against PEDV.</P>

[ICACE2022] Model identification of two double-acting pistons pump

Danh Khoa Nguyen,Cong Toai Truong,Van Tu Duong,Huy Hung Nguyen,Tan Tien Nguyen 한국마린엔지니어링학회 2023 한국마린엔지니어링학회지 Vol.47 No.2

The Covid-19 pandemic has had a comprehensive and far-reaching impact on various fields, especially in the medical aspect. Additionally, many variants of this coronavirus have appeared due to mutation, each of which has distinguished influences. During the Covid-19 pandemic, medical equipment, especially ventilators, has seen a severe shortage. Thus, many studies of ventilators machine have been carried out around the world. And according to these studies, airflow control is one of the critical issues of ventilator machine research that saves human life. Understanding the above problem, it is necessary to conduct system modeling and obtain a model which has high linearity so that the parameters of the ventilation can be controlled to achieve better accuracy. This paper presents the model identification of a two double-acting piston pump system using experimental data. First order plus dead time method is applied to achieve the identification model. The data used for identification and validation are collected by conducting several practical tests on the two double-acting pistons pump system. The discussion on the identified model and future works are also included in this paper.

Structure and Magnetic Properties of Nd45-xCxFe30Co15Al10 Alloys

Nguyen Anh Tuan,Vu Hong Ky,Nguyen Huy Dan,Luu Tien Hung 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

Nd45-xCxFe30Co15Al10 (x = 0, 1, 3, 5 and 7) alloys were prepared by using a copper mold suction casting method. X-ray diraction (XRD) patterns show a trend of increasing crystallinity with increasing C content. In addition, crystallites with dierent shapes and sizes are observed by using scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM). Multi-phase properties of the alloys are clearly exhibited on the magnetic measurements. The hysteresis loops of the lower C content alloys (x = 1 and 3) show a single-phase hard magnetic behavior with coercivities of 6.2 kOe while the loops of the higher C content alloys (x = 5 and 7) are supposing a multi-phase property with coercivities up to 7.2 kOe. Two magnetic-phase transitions, depending on the C concentration, appear in the thermomagnetization curves. Addition of C at low content (1 to 3 at.%) can improve the hard magnetic behavior of the Nd-(Fe, Co)-Al alloy. Nd45-xCxFe30Co15Al10 (x = 0, 1, 3, 5 and 7) alloys were prepared by using a copper mold suction casting method. X-ray diraction (XRD) patterns show a trend of increasing crystallinity with increasing C content. In addition, crystallites with dierent shapes and sizes are observed by using scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM). Multi-phase properties of the alloys are clearly exhibited on the magnetic measurements. The hysteresis loops of the lower C content alloys (x = 1 and 3) show a single-phase hard magnetic behavior with coercivities of 6.2 kOe while the loops of the higher C content alloys (x = 5 and 7) are supposing a multi-phase property with coercivities up to 7.2 kOe. Two magnetic-phase transitions, depending on the C concentration, appear in the thermomagnetization curves. Addition of C at low content (1 to 3 at.%) can improve the hard magnetic behavior of the Nd-(Fe, Co)-Al alloy.

Comparative proteomic analysis of Celastrus hindsii Benth. phenotypes reveals an intraspecific variation

Nguyen, Van Huy,Pham, Thanh Loan,Ha, Thi Tam Tien,Hoang, Thi Le Thu The Korean Society of Plant Biotechnology 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.4

In Vietnam, Celastrus hindsii Benth, a medicinal plant rich in secondary metabolites, has been used to alleviate distress caused by ulcers, tumors, and inflammation for generations. The occurrence of two phenotypes, Broad Leaf (BL) and Narrow Leaf (NL), has raised questions about the selection of appropriate varieties for conservation and crop improvement to enhance medicinal properties. This study examined molecular differences in C. hindsii by comparing protein profiles between the NL and BL types using 2D-PAGE and MS. Peptide sequences and proteins were identified by matching MS data against the MSPnr100 databases and verified using the MultiIdent tool on ExPASy and the Blast2GO software. Our results revealed notable variations in protein abundance between the NL and BL proteomes. Selected proteins were confidently identified from 12 protein spots, thereby highlighting the molecular variation between NL and BL proteomes. Upregulated proteins in BL were found to be associated with flavonoid and amino acid biosynthesis as well as nuclease metabolism, which probably attributed to the intraspecific variations. Several bioactive proteins identified in this study can have applications in cancer therapeutics. Therefore, the BL phenotype characterized by healthier external morphological features has higher levels of bioactive compounds and could be better suited for medicinal use.

Investigation of the Microstructure of Mechanically Milled Nd40Fe30Co15Al10B5 Alloy

Luu Tien Hung,Nguyen Huy Dan 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

The microstructure of Nd40Fe30Co15Al10B5 alloys fabricated by using a high-energy ball-milling method was investigated by using high-resolution transmission electron microscopy (HRTEM), scan- ning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), selected area electron diraction (SAED) and electron backscatter diraction (EBSD) techniques. The crystalline phases found in the alloys were the tetragonal Nd2(Fe,Co,Al)14B (denoted as the 2 : 14 : 1 phase), the tetragonal Nd6(Fe,Co,Al)14 (denoted as the phase) and the fcc Nd-rich (a = 0.51 nm) phases. The formation of 2 : 14 : 1 crystallites supposedly contributed to the high coercivities up to 24 kOe for the alloys. The microstructure of Nd40Fe30Co15Al10B5 alloys fabricated by using a high-energy ball-milling method was investigated by using high-resolution transmission electron microscopy (HRTEM), scan- ning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), selected area electron diraction (SAED) and electron backscatter diraction (EBSD) techniques. The crystalline phases found in the alloys were the tetragonal Nd2(Fe,Co,Al)14B (denoted as the 2 : 14 : 1 phase), the tetragonal Nd6(Fe,Co,Al)14 (denoted as the phase) and the fcc Nd-rich (a = 0.51 nm) phases. The formation of 2 : 14 : 1 crystallites supposedly contributed to the high coercivities up to 24 kOe for the alloys.

Micropropagation from root segments to improve seedling quality in Chinese foxglove crops

Thanh Loan Pham,Van Huy Nguyen,Thi Le Thu Hoang,Thi Tam Tien Ha,Trung Kien Tran,Xuan Duong Vu,Phi Bang Cao,Quang Trung Nguyen 한국식물생명공학회 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.3

This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.

Preparation of g-C3N4/Ta2O5 Composites with Enhanced Visible-Light Photocatalytic Activity

Vo, Vien,Van Kim, Nguyen,Nga, Nguyen Thi Viet,Trung, Nguyen Tien,Giang, Le Truong,Van Hanh, Pham,Hoang, Luc Huy,Kim, Sung-Jin Springer-Verlag 2016 Journal of electronic materials Vol.45 No.5

Novel 3,4-dihydro-4-oxoquinazoline-based acetohydrazides: Design, synthesis and evaluation of antitumor cytotoxicity and caspase activation activity

Huan, Le Cong,Tran, Phuong-Thao,Phuong, Cao Viet,Duc, Phan Huy,Anh, Duong Tien,Hai, Pham The,Huong, Le Thi Thu,Thuan, Nguyen Thi,Lee, Hye Jin,Park, Eun Jae,Kang, Jong Soon,Linh, Nguyen Phuong,Hieu, Tr Elsevier 2019 Bioorganic chemistry Vol.92 No.-

<P><B>Abstract</B></P> <P>In search for novel small molecules with antitumor cytotoxicity via activating procaspase-3, we have designed and synthesized three series of novel (E)-<I>N</I>′-benzylidene-4-oxoquinazolin-3(4<I>H</I>)-yl)acetohydrazides (<B>5a-j, 6a-h,</B> and <B>7a-h)</B>. On the phenyl ring ò the benzylidene part, three different substituents, including 2-OH-4-OCH<SUB>3</SUB>, 4-OCH<SUB>3</SUB>, and 4-N(CH<SUB>3</SUB>)<SUB>2</SUB>, were introduced, respectively. Biological evaluation showed that the acetohydrazides in series <B>5a-j</B>, in which the phenyl ring of the benzylidene part was substituted by 2-OH-4-OCH<SUB>3</SUB> substituent, exhibited potent cytotoxicity against three human cancer cell lines (SW620, colon; PC-3, prostate; NCI-H23, lung). Most of the compounds, in this series, especially compounds <B>5c, 5b</B> and <B>5h,</B> also significantly activated caspase-3 activity. Among these, compound <B>5c</B> displayed 1.61-fold more potent than PAC-1 as caspase-3 activator. Cell cycle analysis showed that compounds <B>5b</B>, <B>5c</B>, and <B>5h</B> significantly arrested the cell cycle in G1 phase. Further apoptotic studies also demonstrated compounds <B>5b</B>, <B>5c</B>, and <B>5h</B> as strong apoptotic cell death inducers. The docking simulation studies showed that these compounds could activate procaspase <I>via</I> chelating Zn<SUP>2+</SUP> ion bound to the allosteric site of the zymogen.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel (<I>E</I>)-<I>N</I>′-benzylidene-2-(4-oxoquinazolin-<I>3(4H)-yl</I>)acetohydrazides were synthesized. </LI> <LI> The acetohydrazides <B>5a-i</B> exhibited potent cytotoxicity against three human cancer cell lines. </LI> <LI> A number of cytotoxic compounds exhibited good caspase activation activity. </LI> <LI> The cytotoxic compounds were shown to arrest cells at G1 phase. </LI> <LI> The cytotoxic compounds were shown as strong apoptotic inducers. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Evaluation of Pt-based alloy/graphene nanohybrid electrocatalysts for triiodide reduction in photovoltaics

Dao, Van-Duong,Larina, Liudmila L.,Tran, Quoc Chinh,Bui, Van-Tien,Nguyen, Van-Toan,Pham, Thanh-Dong,Mohamed, Ibrahim M.A.,Barakat, Nasser A.M.,Huy, Bui The,Choi, Ho-Suk Elsevier 2017 Carbon Vol.116 No.-

<P>This work focuses on systematic studies of dissolution engineering for Pt0.9M0.1/graphene(M = Au, Co, Cu, Fe, Mo, Ni, Pd, Ru, and Sn) counter electrodes (CEs). The developed nanohybrid materials exhibit higher catalytic activity and electrical conductivity compared with those of Pt/graphene CEs. The results also indicate the improved stability of the developed CEs in iodide electrolyte. Furthermore, the trend in the variation of the reactivity of the PtM alloys agrees well with the concept of density functional theory (one-electron description). An enhancement in the catalytic activity of the developed nanohybrids results from the electronic effect that originates from an upward shift of the platinum d-band to the Fermi energy level upon alloying. Thus, the Pt(0.9)M(0.1)graphene nanohybrids are cost-effective alternative CE materials to the expensive Pt. The obtained results provide a foundation for enhancing the catalytic activities of CEs for dye-sensitized solar cells (DSCs). The implementation of the Pt0.9M0.1/graphene nanohybrids offers significant potential for increasing the efficiency of DSCs. (C) 2017 Elsevier Ltd. All rights reserved.</P>