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Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Verification of Therapeutic Effect of a Piston Device for Foot with Spastic Paralysis
Nguyen Thi Kieu Chinh,Hirofumi Tanabe,Kenji Ooka,Yoshifumi Morita,Yukio Kobayashi,Takafumi Ijiri 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10
The second author has developed a piston technique for restoring the motor function of a chronic plegic foot with good outcomes, including reduced spasticity and improved muscle shortening. In this study, we developed a piston device for foot (PDFt) to simulate the piston technique. The PDFt moves the paralyzed foot passively. We considered the therapeutic effects as well as new findings in treatment with the PDFt. We conducted treatment with the PDFt for five minutes on each of six hemiplegic persons. The therapeutic effect was assessed by measuring the passive range of motion, the active range of motion, and the Modified Ashworth Scale score before and after treatment. The use of the PDFt led to improved motor function and decreased abnormal muscle tone. Therefore, the effectiveness of treatment with the PDFt was verified. Moreover, we conducted a 5-Meter Walk Test before and after treatment for one hemiplegic person and performed a gait analysis. It was confirmed that the treatment also had a good effect on the improvement of gait.