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Nagai Ryo,Ebihara Takeru,Kakino Kohei,Masuda Akitsu,Xu Jian,Minamihata Kosuke,Kamiya Noriho,Kongkrongtong Tatphon,Kawahara Masahiro,Mon Hiroaki,Fujii Tsuguru,Kusakabe Takahiro,Lee Jae Man 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
Due to the biological significance and therapeutic potential of Interleukin-3 (IL-3) secreted mainly by activated T cells, various protein expression systems have been challenged to produce recombinant IL3 to meet the increasing demands worldwide. Recently, we established an updated silkworm-based baculovirus expression vector system (silkworm-BEVS), which in most cases, produces eukaryotic proteins in biological or enzymatical active forms with considerable amounts. We attempted to reconstruct and express a recombinant mouse IL-3 (rMmIL-3) with C-terminal His8-Strep tags in silkworm-BEVS in the current study. From our results, we gained an active glycosylated rMmIL-3 protein in a substantial amount and quality. As compared with the E. coli expression system, silkworm-BEVS is a better choice regarding the glycosylations attached in rMmIL-3 and upscalable system in case that a commercial amount is required in the future. Collectively, our method shares an excellent model to produce interleukin molecular for approaching pharmaceutical applications.
A system for three-dimensional gaze fixation analysis using eye tracking glasses
Ryo Takahashi,Hiromasa Suzuki,Jouh Yeong Chew,Yutaka Ohtake,Yukie Nagai,Koichi Ohtomi 한국CDE학회 2018 Journal of computational design and engineering Vol.5 No.4
Eye tracking is a technology that has quickly become a commonplace tool for evaluating package and webpage design. In such design processes, static two-dimensional images are shown on a computer screen while a subject’s gaze where he or she looks is measured via an eye tracking device. The collected gaze fixation data are then visualized and analyzed via gaze plots and heat maps. Such evaluations using two-dimensional images are often too limited to analyze gaze on three-dimensional physical objects such as products because users look at them not from a single point of view but rather from various angles. Therefore in this study we propose methods for collecting gaze fixation data for a three-dimensional model of a given product and visualizing corresponding gaze plots and heat maps also in three dimen-sions. To achieve our goals, we used a wearable eye-tracking device, i.e., eye-tracking glasses. Further, we implemented a prototype system to demonstrate its advantages in comparison with two-dimensional gaze fixation methods.