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Actinomycotic Osteomyelitis of the Mandible: A Case Report
Lee, Young-Cheol,Lim, Lee-Rang,Lee, Kyu-Hoon,Seo, Dong-Jun,Yun, Na-Ra,Oh, Ji-Su,You, Jae-Seek,Choi, Hae-In Korean Academy of Orofacial Pain and Oral Medicine 2019 Journal of Oral Medicine and Pain Vol.44 No.3
Actinomycosis is rare, chronic, slowly progressive disease caused by gram-positive anaerobic organisms from the Actinomycosis family that normally colonizes the oral cavity. Actinomycotic osteomyelitis is even more rare and refractory disease because diagnosis by bacterial culture is not easy. In our case, 80-year-old man visited our clinic with a complaint of swelling and severe sinus tracts without teeth evolvement on anterior mandible. Computed tomography (CT) scan demonstrated erosive bone destruction on anterior mandible, clinically suspected actinomycotic osteomyelitis. The patient also had thoracic actinomycosis on Lt. lower lung. We could diagnosis actinomycosis by histopathologic examination. He treated by conservative surgery and long term antibiotics. After 2 year, no recurrence was seen in CT scan.
A Case Report on the Risk of Enterobacteriaceae Infection in the Oral and Maxillofacial Region
Lim, Lee-Rang,Lee, Young-Cheol,Lee, Hye-Jung,Jung, Gyeo-Woon,Yun, Na-Ra,Seo, Yo-Seob,Oh, Ji-Su,You, Jae-Seek Korean Academy of Orofacial Pain and Oral Medicine 2019 Journal of Oral Medicine and Pain Vol.44 No.3
Osteomyelitis is an inflammatory condition of the bone caused by pathogenic bacteria. The causative pathogen is usually oral residing bacteria, but this is a report of patients with osteomyelitis infected with Enterobacteriaceae, which is not common. Enterobacteriaceae has been known to cause in-hospital infections for over last 30 years and is known to have multiple antibiotic resistances. Both cases in this study developed osteomyelitis after removal of the dentigerous cyst. Enterobacter aerogenes was cultured in one patient and Serratia marcescens in the other. After changing antibiotics through antibiotic susceptibility testing, clinical symptoms subsided and radiographic images confirmed that the callus formed and recovered at the same time.
( Yi Rang Na ),( Seung Hyeok Seok ),( Min Won Baek ),( Hui Young Lee ),( Dong Jae Kim ),( Sung Hoon Park ),( Hyun Kyoung Lee ),( Byoung Hee Lee ),( Jae Hak Park ) 한국동물실험대체법학회 2007 한국동물실험대체법학회 학술대회집 Vol.2007 No.1
Pro-oxidant 3,3`,4,4`,5-pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. Disruption from cellular oxidative stress can be blocked by several nutrient antioxidants like vitamin E. We used the fish model, zebrafish (Danio rerio), to determine if vitamin E can protect against toxicity from the aryl hydrocarbon receptor (AHR) ligand PCB126 during zebrafish development, and to show the mechanism by which PCB 126 induces toxic effects. We exposed zebrafish embryos at 8 hours post fertilization (hpf) to PCB126 at a concentration of 100 nM, and compared these fish with a second group that was co-exposed to 100-μM vitamin E and 100-nM PCB126 until 5 days post fertilization (dpf). PCB126 induced pericardial sac edema, yolk sac edema, heart malformation, hemorrhaging, failure of swim bladder inflation, growth retardation and mortality in developing zebrafish larvae. In contrast, 100-μM vitamin E co-exposure did not induce the gross changes seen in the PCB126 treatment group. We found that vitamin E can protect against PCB126 toxicity by inhibiting zebrafish heat shock protein 70 cognate (zfHsc70) down regulation. Also vitamin E inhibited the zebrafish aryl hydrocarbon receptor type-2 (zfAHR2), zebrafish cytochrome P450 1A (zfCYP1A), zebrafish superoxide dismutase-1 (zfSOD1) and upregulated zebrafish superoxide dismutase-2 (zfSOD2) mRNA expression. These data give insights into the use of vitamin E to reduce PCB126-mediated toxicity and into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of AHR agonists.
Eun Na Lee,Jin Kyeong Park,Ja-Rang Lee,Sae-Ock Oh,Sun-Yong Baek,Bong-Seon Kim,Sik Yoon 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.1
The thymus is a central lymphoid organ for T cell development. Thymic epithelial cells (TECs) constitute a major component of the thymic stroma, which provides a specialized microenvironment for survival, proliferation, and differentiation of immature T cells. In this study, subsets of TECs were examined immunohistochemically to investigate their cytokeratin (CK) expression patterns during thymus regeneration following thymic involution induced by cyclophosphamide treatment. The results demonstrated that both normal and regenerating mouse thymuses showed a similar CK expression pattern. The major medullary TECs (mTEC) subset, which is stellate in appearance, exhibited CK5 and CK14 staining, and the minor mTEC subset, which is globular in appearance, exhibited CK8 staining, whereas the vast majority of cortical TECs (cTECs) expressed CK8 during thymus regeneration. Remarkably, the levels of CK5 and CK14 expression were enhanced in mTECs, and CK8 expression was upregulated in cTECs during mouse thymus regeneration after cyclophosphamide-induced acute thymic involution. Of special interest, a relatively high number of CK5+CK8+ TEC progenitors occurred in the thymic cortex during thymus regeneration. Taken together, these findings shed more light on the role of CK5, CK8, and CK14 in the physiology of TECs during mouse thymus regeneration, and on the characterization of TEC progenitors for restoration of the epithelial network and for concomitant regeneration of the adult thymus.
B16F10 멜라노마세포에서 과기음가미방의 멜라닌 생성 촉진 효과
문나랑 ( Na Rang Moon ),김세윤 ( Se Yoon Kim ),이진혁 ( Jin Hyuk Lee ),이정복 ( Jung Bok Lee ),박선민 ( Sun Min Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.3
Objectives : Since hypopigmentation is known to increase the risk of skin cancer, melanogenesis in the skin needs to be regulated. Here, we evaluated the melanogenesis stimulatory effects of a modified Goagium herbal remedy (HR) and HR+ox bile (Bos taurus domesticus) extract (OBE) to address hypopigmentation disorders. Methods : B16F10 melanoma cells were treated with different dosages of HR and HR+OBE for 24 to 48 h after 1 h of 10 nM α-melanocyte stimulating hormone (α-MSH). After the treatment, cell viability, tyrosinase activity, melanin synthesis and the expression of genes related to melanin synthesis were measured and the regulation of the α-MSH signalling through cAMP responding element binding protein (CREB) was determined. Results : HR and HR+OBE with the ranges of 15~100 μg/mL did not affect cell viability in melanoma cells. The 1 h treatment of HR+OBE (50 and 100 μg/mL) potentiated the phosphorylation of CREB by enhancing α -MSH signaling and its 24 h treatment increased CREB expression. Consistent with CREB potentiation, their treatment for 24 h, the expression of microphthalmia-associated transcription factor (MIFT), tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 were increased in realtime PCR. Ultimately, the 48 h treatment of HR+OBE (50 and 100 μg/mL) increased tyrosniase activity and melanin contents in the melanoma cells in comparison to the control. Conclusions : HR+OBE (50 and 100 μg/mL) increases melanin synthesis in B16F10 melanoma cells via the stimulation of tyrosinase activity and expression of MIFT, tyrosinase, TRP-1 and TRP-2. HR+OBE can be used as the a possible treatment for hypopigmentation of the skin.
Eun Na Lee,Jin Kyeong Park,Ja-Rang Lee,Sae-Ock Oh,Sun-Yong Baek,Bong-Seon Kim,Sik Yoon 대한해부학회 2010 Anatomy & Cell Biology Vol.43 No.1
The thymus is a central lymphoid organ for T cell development. Thymic epithelial cells (TECs) constitute a major component of the thymic stroma, which provides a specialized microenvironment for survival, proliferation, and differentiation of immature T cells. In this study, subsets of TECs were examined immunohistochemically to investigate their cytokeratin (CK) expression patterns during thymus regeneration following thymic involution induced by cyclophosphamide treatment. The results demonstrated that both normal and regenerating mouse thymuses showed a similar CK expression pattern. The major medullary TECs (mTEC) subset, which is stellate in appearance, exhibited CK5 and CK14 staining, and the minor mTEC subset, which is globular in appearance, exhibited CK8 staining, whereas the vast majority of cortical TECs (cTECs) expressed CK8 during thymus regeneration. Remarkably, the levels of CK5 and CK14 expression were enhanced in mTECs, and CK8 expression was upregulated in cTECs during mouse thymus regeneration after cyclophosphamide-induced acute thymic involution. Of special interest, a relatively high number of CK5+CK8+ TEC progenitors occurred in the thymic cortex during thymus regeneration. Taken together, these findings shed more light on the role of CK5, CK8, and CK14 in the physiology of TECs during mouse thymus regeneration, and on the characterization of TEC progenitors for restoration of the epithelial network and for concomitant regeneration of the adult thymus.
Enzyme-Linked Immunosorbent Assay to Detect the MHV Infection in Mice
Yi-Rang Na,Seung-Hyeok Seok,Min-Won Baek,Hui-Young Lee,Dong-Jae Kim,Kyoung-Jin Noh,Sung-Hoon Park,Hyun-Kyoung Lee,Noton Kumar Dutta,Byoung-Hee Lee,Jae-Hak Park 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.4
Mouse hepatitis virus (MHV) is one of the major troublesome infectious diseases in laboratory mice. ELISA techniques generally have been shown to be more sensitive than other diagnostic methods for detection of MHV infection. Here, we developed an ELISA test method by using MHV type-2 strain and it gave reliable test results about detection of MHV infection in mice with high accuracy and low costs.
Establishment of the Neutral Red Uptake Assay as Alternatives to the Draize test and its Validation
( Hyoun Kyoung Lee ),( Kyoung Jin Noh ),( Seung Hyeok Seok ),( Min Won Baek ),( Hui Young Lee ),( Dong Jae Kim ),( Yi Rang Na ),( Sung Hoon Park ),( Dutta Noton Kumar ),( Byoung Hee Lee ),( Bae Hwan K 한국동물실험대체법학회 2007 한국동물실험대체법학회 학술대회집 Vol.2007 No.1
The necessity of using animals to test whether new chemicals and products are eye irritants has been questioned over the last 20 years. During this process, numerous non-animal methods have been proposed as reliable alternatives to the Draize test. The neutral red uptake assay is the in vitro method to test the eye irritation of chemicals and products. We need to validate the neutral red uptake assay as alternatives for the international harmonization. Therefore, we studied for the establishment of the neutral red uptake assay in Korea. In this study, we evaluated the eye irritation of five chemicals and three cosmetics by the Draize test and the neutral red uptake assay. The results of the neutral red uptake assay showed a correlation of r=−0.893(p=0.107) with our Draize score in two chemicals. And the results of the neutral red uptake assay showed correlation of r=0.892(p=0.017) with the Draize score obtained from the ECVAM (European Centre for the Validation of Alternative Methods) in additional three chemicals. All of three cosmetics were evaluated as non-irritants by our Draize test and the neutral red uptake assay. As a result, the neutral red uptake assay was established in Korea. For the international harmonization, the validation study has to be performed with more chemicals and products in more countries.