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      저자 : NICOLAS G. GREEN (검색결과 3 건)
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      • KCI등재후보

        ANALYTICAL AND NUMERICAL MODELING METHODS FOR IMPEDANCE ANALYSIS OF SINGLE CELLS ON-CHIP

        TAO SUN,NICOLAS G. GREEN,HYWEL MORGAN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.1

        Electrical impedance spectroscopy (EIS) is a noninvasive method for characterizing the dielectric properties of biological particles. The technique can differentiate between cell types and provide information on cell properties through measurement of the permittivity and conductivity of the cell membrane and cytoplasm. In terms of lab-on-a-chip (LOC) technology, cells pass sequentially through the microfluidic channel at high speed and are analyzed individually, rather than as traditionally done on a mixture of particles in suspension. This paper describes the analytical and numerical modeling methods for EIS of single cell analysis in a microfluidic cytometer. The presented modeling methods include Maxwell's mixture theory, equivalent circuit model and finite element method. The difference and advantages of these methods have been discussed. The modeling work has covered the static case — an immobilized cell in suspension and the dynamic case — a moving cell in the channel.

      • KCI등재후보

        High speed simultaneous single particle impedance and fluorescence analysis on a chip

        Hywel Morgan,David Holmes,Nicolas G. Green 한국물리학회 2006 Current Applied Physics Vol.6 No.3

        ticles at high speed. The device uses multi-frequency electrical impedance together with single particle uorescence spectroscopy. Imped-ance is measured using microelectrodes fabricated within a microuidic channel. Optical measurements are made by focussing a beam oflight into a detection volume of the order of the size of the particle. Particles owing through the device are dynamically focussed into thedetection volume using dielectrophoresis. The operating principle of the device is demonstrated by detecting and analysing uorescent.

      • An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement.

        Wang, Kuan-Chih,Kumar, Aloke,Williams, Stuart J,Green, Nicolas G,Kim, Kyung Chun,Chuang, Han-Sheng Royal Society of Chemistry 2014 Lab on a chip Vol.14 No.20

        <P>Technologies that can enable concentration of low-abundance biomarkers are essential for early diagnosis of diseases. In this study, an optoelectrokinetic technique, termed Rapid Electrokinetic Patterning (REP), was used to enable dynamic particle manipulation in bead-based bioassays. Various manipulation capabilities, such as micro/nanoparticle aggregation, translation, sorting and patterning, were developed. The technique allows for versatile multi-parameter (voltage, light intensity and frequency) based modulation and dynamically addressable manipulation with simple device fabrication. Signal enhancement of a bead-based bioassay was demonstrated using dilute biotin-fluorescein isothiocyanate (FITC) solutions mixed with streptavidin-conjugated particles and rapidly concentrated with the technique. As compared with a conventional ELISA reader, the REP-enabled detection achieved a minimal readout of 3.87 nM, which was a 100-fold improvement in sensitivity. The multi-functional platform provides an effective measure to enhance detection levels in more bead-based bioassays.</P>

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