D상 유화물을 이용한 W/O 유화물의 제조와 특성

남기대,김형진,정노희,윤영균,박관선 한국유화학회 1998 한국응용과학기술학회지 Vol.15 No.2

The emulsion stability of W/O emulsion prepared by D phase emulsification during storage and handling is studied by using phase diagrams. The process of D phase emulsification begins with the formation of isotropic surfactant solution, followed by formation of oil-in-surfactant (O/D) gel emulsion by dispersion of octamethylcyclotetrasiloxane(OMCS) in the surfactant solution. Polyols were essential components for this purpose. To understand the function of polyols, the solution behavior of nonionic surfactant/oil/water/polyol systems were investigated by the ternary phase diagrams of polyoxyethylene loeyl ether/OMCS/propylene glycol(PG) aqueous solutions. The addition of PG increased the solubility of oil in the isotropic surfactant phase. D phase emulsification method has been applied to a new type of cosmetics. By using this emulsification technique, O/W emulsion were formed without a need for adjust of HLB. Fine and stable W/O emulsions were prepared by D phase emulsion.

Purification and characterization of an antimicrobial histone H1-like protein and its gene from the testes of olive flounder, Paralichthys olivaceus

Nam, B.H.,Seo, J.K.,Go, H.J.,Lee, M.J.,Kim, Y.O.,Kim, D.G.,Lee, S.J.,Park, N.G. Academic Press 2012 Fish & Shellfish Immunology Vol.33 No.1

An approximately 21 kDa antimicrobial protein was purified from an acidified testis extract of olive flounder, Paralichthys olivaceus, by ion-exchange and C<SUB>18</SUB> reversed-phase HPLC. A comparison of the N-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone H1 molecules; thus, it was designated flounder histone H1-like protein (fH1LP). fH1LP showed potent antimicrobial activity against Gram-positive bacteria, including Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 2.8-30.0 μg/ml), Gram-negative bacteria, including Aeromonas hydrophila, Escherichia coli D31, Vibrio parahaemolyticus (MECs, 1.4-12.0 μg/ml), and Candida albicans (MEC, 2.0 μg/ml). cDNA cloning and tissue distribution studies of fH1LP indicated that it is constitutively expressed in testis and ovary. The fH1LP expression level was significantly dependent on developmental stage, and decreased dramatically after hatching. However, lipopolysaccharide stimulation did not induce fH1LP mRNA in other immune organs, including the kidney and spleen. These results suggest that fH1LP plays an important role in innate immunity in fish during reproduction, including mating, fertilization, and hatching.

Exploration of novel 5'(7')-substituted-2'-oxospiro[1,3]dioxolane-2,3'-indoline-based N-hydroxypropenamides as histone deacetylase inhibitors and antitumor agents

Dung, D.T.M.,Dung, P.T.P.,Oanh, D.T.K.,Vu, T.K.,Hahn, H.,Han, B.W.,Pyo, M.,Kim, Y.G.,Han, S.B.,Nam, N.H. Elsevier 2017 Arabian journal of chemistry Vol.10 No.4

<P>A series of novel 5'(7')-substituted-2'-oxospiro[1,3] dioxolane-2,3'-indoline-based N-hydroxypropenamides were designed, synthesized and evaluated for histone deacetylase (HDAC) inhibition and cytotoxicity. It was found that the compounds in this series displayed potent inhibitory effects against HDAC2 with IC50 values as low as 0.284 mu M, almost comparable to that of SAHA (IC50, 0.265 mu M), a positive control. In Western blot analysis, these compounds also exhibited noted inhibition toward histone deacetylation and this inhibition was found to correlate well with the cytotoxicity of the compounds in three human cancer cell lines. Docking studies indicated the compounds in this series bound to HDAC2 with high binding affinities (similar to -9.8 kcal/mol) compared to SAHA (-7.4 kcal/mol). (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.</P>

A homolog of teleostean signal transducer and activator of transcription 3 (STAT3) from rock bream, <i>Oplegnathus fasciatus</i>: Structural insights, transcriptional modulation, and subcellular localization

Bathige, S.D.N.K.,Thulasitha, William Shanthakumar,Umasuthan, Navaneethaiyer,Jayasinghe, J.D.H.E.,Wan, Qiang,Nam, Bo-Hye,Lee, Jehee Elsevier 2017 Veterinary immunology and immunopathology Vol.186 No.-

<P><B>Abstract</B></P> <P>Signal transducer and activator of transcription 3 (STAT3) is one of the crucial transcription factors in the Janus kinase (JAK)/STAT signaling pathway, and it was previously considered as acute phase response factor. A number of interleukins (ILs) such as IL-5, IL-6, IL-9, IL-10, IL-12, and IL-22 are known to be involved in activation of STAT3. In addition, various growth factors and pathogenic or oxidative stresses mediate the activation of a wide range of functions via STAT3. In this study, a STAT3 homolog was identified and functionally characterized from rock bream (RbSTAT3), <I>Oplegnathus fasciatus</I>. <I>In silico</I> characterization revealed that the RbSTAT3 amino acid sequence shares highly conserved common domain architectural features including N-terminal domain, coiled coil domain, DNA binding domain, linker domain, and Src homology 2 (SH2) domains. In addition, a fairly conserved transcriptional activation domain (TAD) was located at the C-terminus. Comparison of RbSTAT3 with other counterparts revealed higher identities (>90%) with fish orthologs. The genomic sequence of <I>RbSTAT3</I> was obtained from a bacterial artificial chromosome (BAC) library, and was identified as a multi-exonic gene (24 exons), as found in other vertebrates. Genomic structural comparison and phylogenetic studies have showed that the evolutionary routes of teleostean and non-teleostean vertebrates were distinct. Quantitative real time PCR (qPCR) analysis revealed that the spatial distribution of <I>RbSTAT3</I> mRNA expression was ubiquitous and highly detectable in blood, heart, and liver tissues. Transcriptional modulation of <I>RbSTAT3</I> was examined in blood and liver tissues after challenges with bacteria (<I>Edwardsiella tarda</I> and <I>Streptococcus iniae</I>), rock bream irido virus (RBIV), and immune stimulants (LPS and poly (I:C)). Significant changes in <I>RbSTAT3</I> transcription were also observed in response to tissue injury. In addition, the transcriptional up-regulation of <I>RbSTAT3</I> was detected in rock bream heart cells upon recombinant rock bream IL-10 (rRbIL-10) treatment. Subcellular localization and nuclear translocation of rock bream STAT3 following poly (I:C) treatment were also demonstrated. Taken together, the results of the current study provide important evidence for potential roles of rock bream STAT3 in the immune system and wound healing processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Identification of STAT3 (RbSTAT3) ortholog from rock bream. </LI> <LI> Genomic structure and putative promoter analysis of <I>RbSTAT3</I>. </LI> <LI> Expression profile (<I>In vivo</I>) of <I>RbSTAT3</I> upon pathogenic and PAMP stress, and tissue injury. </LI> <LI> Expression profile (<I>In vitro</I>) of <I>RbSTAT3</I> in response to rock bream IL-10. </LI> <LI> Subcellular localization of RbSTAT3. </LI> </UL> </P>

온간상태에서 비틀림변형에 의한 Cementite 의 구상화

남승의,이동녕 대한금속재료학회(대한금속학회) 1981 대한금속·재료학회지 Vol.19 No.2

Spheroidization of cementite in eutectoid steel by concurrent warm torsional deformation has been studied with emphasis of the effects of the strain rate and the inter-lamellar spacing. Complete spheroidization could be achieved in 3 minutes when the strain rate and the deformation temperature were 1.76 percent/sec 700℃ respectively. The rate of the sphsroidization increased with increasing temperature at a given strain rate and lameller spacing. The coarse pearlite was more easily spheroidizad than the fine pearlite when other conditions were same.

알파술폰 고급지방산 폴리에틸렌 글리콜 에스테르류의 합성

남기대,김진현,연영흠,정노희 한국유화학회 1998 한국응용과학기술학회지 Vol.15 No.2

α-sulfonated fatty acid polyethylene glycol esters with polyethylene oxide(addition, 3, 5, 10mol) were synthesized through esterification of α-sulfonated fatty acid methyl esters with alkyl chain length C_12∼C_18. Their compounds were separated with column chromatography, and confirmed by TLC. Quantitative analysis of all the sulfonates were performed according to JIS K-3362 method, and ethylene oxide unit number were determined by ISO 2270 method. Structural properties of α-sulfonated fatty acid methyl esters and their derivatives were also identified from IR. and ^1H NMR spectra.

Thermosyphon의 가열량 증가시의 온도특성에 관한 연구

하옥남,박찬수,남동현 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

The research is focused on the stability in which the incoming heat to the evaporator is in creased and transfer capability which is required in designing of a system. First, in case the incoming heat to the thermosyphon evaporator is increased and the quantity of operational fluid is less than the requirement, the surface temperature of evaporator increases, which can make the system abnormal and cause damage to the system to be out of order. Therefore, the aim of this research is to study the characteristics in which the heating amount of the thermosyphon is increased.

Transcriptional profiling, molecular cloning, and functional analysis of C1 inhibitor, the main regulator of the complement system in black rockfish, <i>Sebastes schlegelii</i>

Nilojan, Jehanathan,Bathige, S.D.N.K.,Thulasitha, W.S.,Kwon, Hyukjae,Jung, Sumi,Kim, Myoung-Jin,Nam, Bo-Hye,Lee, Jehee Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.75 No.-

<P><B>Abstract</B></P> <P>C1-inhibitor (C1inh) plays a crucial role in assuring homeostasis and is the central regulator of the complement activation involved in immunity and inflammation. A C1-inhibitor gene from <I>Sebastes schlegelii</I> was identified and designated as <I>SsC1inh</I>. The identified genomic DNA and cDNA sequences were 6837 bp and 2161 bp, respectively. The genomic DNA possessed 11 exons, interrupted by 10 introns. The amino acid sequence possessed two immunoglobulin-like domains and a serpin domain. Multiple sequence alignment revealed that the serpin domain of SsC1inh was highly conserved among analyzed species where the two immunoglobulin-like domains showed divergence. The distinctiveness of teleost C1inh from other homologs was indicated by the phylogenetic analysis, genomic DNA organization, and their extended N-terminal amino acid sequences. Under normal physiological conditions, <I>SsC1inh</I> mRNA was most expressed in the liver, followed by the gills. The involvement of SsC1inh in homeostasis was demonstrated by modulated transcription profiles in the liver and spleen upon pathogenic stress by different immune stimulants. The protease inhibitory potential of recombinant SsC1inh (rSsC1inh) and the potentiation effect of heparin on rSsC1inh was demonstrated against C1esterase and thrombin. For the first time, the anti-protease activity of the teleost C1inh against its natural substrates C1r and C1s was proved in this study. The protease assay conducted with recombinant black rockfish C1r and C1s proteins in the presence or absence of rSsC1inh showed that the activities of both proteases were significantly diminished by rSsC1inh. Taken together, results from the present study indicate that SsC1inh actively plays a significant role in maintaining homeostasis in the immune system of black rock fish.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A C1 inhibitor gene was identified from Black rockfish with serpin features. </LI> <LI> Genomic DNA was made up of 11 exons and 10 introns. </LI> <LI> Modulated transcriptional patterns were observed after immune stimulation. </LI> <LI> Antiprotease activity of SsC1inh is enhanced by the addition of heparin. </LI> <LI> SsC1inh significantly diminished the activity of SsC1r and SsC1s. </LI> </UL> </P>

Systems for Production of Calves from Hanwoo(Korean Cattle) IVM/IVF/IVC Blastocyst III. Vitrification and One-Step Dilution of Hanwoo Blastocyst

Kim, E.Y.,Kim, D.I.,Park, N.H.,Weon, Y.S.,Nam, H.K.,Lee, K.S.,Park, S.Y.,Yoon, S.H.,Park, S.P.,Chung, K.S.,Lim, J.H. The Korean Society of Animal Reproduction 1999 Reproductive & developmental biology Vol.23 No.4

This study was to examine whether Hanwoo IVM/IVF/IVC blastocyst can be successfully survived in vitro/in vivo after vitrification and one-step dilution. For vitrification, blastocysts were serially exposed in glycerol (G) and ethylene glycol(EG) mixtures[10% (v Iv) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then they were loaded in the straw, placed in cold nitrogen vapor for 3 min. and plunged into L$N_2$(-196$^{\circ}C$). One-step dilution within the straw was done in $25^{\circ}C$ and 36$^{\circ}C$ water for about 5 min. and 3 min., respectively. Recovered embryos after one-step dilution were cultured in cumulus cell mono-layered drop for 48 h or were transferred into recipient cows. When the embryo survival in vitro was assessed to re-expanded and hatched rates at 24 hand 48 h after one-step dilution, the results of vitrified group (85.4, 43.8%) was high, although these results were significantly lower than normal development (100.0, 63.3%) of control group, respectively (P<0.001, P<0.05). When in vitro survival of vitrified groups according to developmental stage was compared, the results of fast developed embryos (expanded blastocyst and early hatching blastocyst stage) were significantly higher than those of delayed developed one (early blastocyst stage) after one-step dilution (early hatching: 88.0, 48.0%: expanded: 81.1, 45.3%; early: 66.7, 14.3%) (P<0.05). Also, in case of in vitro survival of vitrified groups according to embryo age (day 7, 8 and 9), when embryo age was younger, in vitro survival was significantly higher (day 7: 67.3, 34.5%; day 8: 76.9, 40.7%; day 9: 60.9, 23.9%)(P<0.05). Finally, when in vivo development potential of vitrified and one-step diluted Hanwoo blastocysts was examined, 4 of 8 recipient (50%) cows became confirmed pregnant. These results demonstrated that our vitrification and one-step dilution technique can be applied easily and effectively on field trial without the equipment and embryological skills required for conservative dilution and transfer.

Designing thermal and electrochemical oxidation processes for d-MnO2 nanofibers for high-performance electrochemical capacitors

Lee, J. H.,Yang, T. Y.,Kang, H. Y.,Nam, D. H.,Kim, N. R.,Lee, Y. Y.,Lee, S. H.,Joo, Y. C. Royal Society of Chemistry 2014 Journal of Materials Chemistry A Vol.2 No.20