Investigation of antimicrobial activity and entA and entB genes in Enterococcus faecium and Enterococcus faecalis strains isolated from naturally fermented Turkish white cheeses

Togay, Sine Ozmen,Ay, Mustafa,Guneser, Onur,Yuceer, Yonca Karagul 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

In this research, the antagonistic effects of Enterococcus faecalis and E. faecium bacteria isolated from naturally fermented Turkish white cheeses, produced without starter culture, against Listeria monocytogenes, L. innocua, L. ivanovii, Staphylococcus aureus, and E. faecalis were evaluated. The presence of entA and entB genes was also detected in the isolates, which had antimicrobial activity. Total 71 strains of E. faecalis and 7 strains of E. faecium were tested; 20 of E. faecalis and none of E. faecium strains showed antimicrobial activity against the tested bacteria using agar spot method. Among E. faecalis strains, which had antimicrobial activity, three strains contained both entA and entB genes, two strains carried only entA gene, and five strains had only entB gene. These cheese-sourced enterococcal strains or their enterocins should be considered to be used for food preservation especially in the dairy industry.

Medicinal leech therapy—an overall perspective

Ali K. Sig,Mustafa Guney,Aylin Uskudar Guclu,Erkan Ozmen 한국한의학연구원 2017 Integrative Medicine Research Vol.6 No.4

Complementary medicine methods have a long history, but modern medicine has just recently focused on their possible modes of action. Medicinal leech therapy (MLT) or hirudotherapy, an old technique, has been studied by many researchers for possible effects on various diseases such as inflammatory diseases, osteoarthritis, and after different surgeries. Hirudo medicinalis has widest therapeutic usage among the leeches, but worldwide, many different species were tested and studied. Leeches secrete more than 20 identified bioactive substances such as antistasin, eglins, guamerin, hirudin, saratin, bdellins, complement, and carboxypeptidase inhibitors. They have analgesic, anti-inflammatory, platelet inhibitory, anticoagulant, and thrombin regulatory functions, as well as extracellular matrix degradative and antimicrobial effects, but with further studies, the spectrum of effects may widen. The technique is cheap, effective, easy to apply, and its modes of action have been elucidated for certain diseases. In conclusion, for treatment of some diseases, MLT is not an alternative, but is a complementary and/or integrative choice. MLT is a part of multidisciplinary treatments, and secretes various bioactive substances. These substances vary among species and different species should be evaluated for both treatment capability and their particular secreted molecules. There is huge potential for novel substances and these could be future therapeutics.

An Experimental and Numerical Investigation of Particle Morphology Effect on the Elasto-plastic Behavior of Particle-filled Composites

Kenan Cinar,Sunal Ahmet Parasiz,Mustafa Akbulut,S. Ozmen Eruslu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9

In this study, the influence of particle shapes on the elastoplastic response of composite materials is investigatedboth experimentally and computationally. Flake, spherical, irregular, and rod type glass particles are used as filler in theepoxy matrix material in various mass fractions: 5, 10, 15 %. For each investigated composite material, mechanicalproperties such as elastic modulus, tensile strength, and tensile fracture strain are determined by experimentally obtainedstress-strain curves. Flake and irregular particle types give the maximum and minimum elastic modulus values, respectively. The addition of glass particles has an adverse effect on the tensile strength and tensile fracture strain. The worst filling typesin terms of tensile strength and tensile fracture strain are the flake and the irregular ones due to the stress concentrations at thesharp edges and corners present on these filler particles under loading conditions. On the otherhand, for spherical type fillersthat have no sharp corners, debonding seem to be the main failure mechanism. Representative volume element (RVE)approach is utilized to determine experimentally identified properties by means of Finite Element Method. In the model, thedebonding among particle and matrix components is simulated through the cohesive zone model (CZM). Computationaloutputs are quite well compliant with experimental results. Experimental results and computational analysis indicate thatalong with the aspect ratio, some other morpohological characteristics of particles have a marked effect on the mechanicalproperties of the particle filled composites, such as presence of sharp corners and edges on the particle geometry and surfacearea to volume ratio of the particles.

Detection of PIK3CA Gene Mutations with HRM Analysis and Association with IGFBP-5 Expression Levels in Breast Cancer

Dirican, Ebubekir,Kaya, Zehra,Gullu, Gokce,Peker, Irem,Ozmen, Tolga,Gulluoglu, Bahadir M.,Kaya, Handan,Ozer, Ayse,Akkiprik, Mustafa Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Breast cancer is the second most common cancer and second leading cause of cancer deaths in women. Phosphatidylinositol-3-kinase (PI3K)/AKT pathway mutations are associated with cancer and phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) gene mutations have been observed in 25-45% of breast cancer samples. Insulin growth factor binding protein-5 (IGFBP-5) can show different effects on apoptosis, cell motility and survival in breast cancer. We here aimed to determine the association between PIK3CA gene mutations and IGFBP-5 expressions for the first time in breast cancer patients. Frozen tumor samples from 101 Turkish breast cancer patients were analyzed with high resolution melting (HRM) for PIK3CA mutations (exon 9 and exon 20) and 37 HRM positive tumor samples were analyzed by DNA sequencing, mutations being found in 31. PIK3CA exon 9 mutations (Q546R, E542Q, E545K, E542K and E545D) were found in 10 tumor samples, exon 20 mutations (H1047L, H1047R, T1025T and G1049R) in 21, where only 1 tumor sample had two exon 20 mutations (T1025T and H1047R). Moreover, we detected one sample with both exon 9 (E542Q) and exon 20 (H1047R) mutations. 35% of the tumor samples with high IGFBP-5 mRNA expression and 29.4% of the tumor samples with low IGFBP-5 mRNA expression had PIK3CA mutations (p=0.9924). This is the first study of PIK3CA mutation screening results in Turkish breast cancer population using HRM analysis. This approach appears to be a very effective and reliable screening method for the PIK3CA exon 9 and 20 mutation detection. Further analysis with a greater number of samples is needed to clarify association between PIK3CA gene mutations and IGFBP-5 mRNA expression, and also clinical outcome in breast cancer patients.

Factors Predicting Microinvasion in Ductal Carcinoma in situ

Ozkan-Gurdal, Sibel,Cabioglu, Neslihan,Ozcinar, Beyza,Muslumanoglu, Mahmut,Ozmen, Vahit,Kecer, Mustafa,Yavuz, Ekrem,Igci, Abdullah Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Background: Whether sentinel lymph node biopsy (SLNB) should be performed in patients with pure ductal carcinoma in situ (DCIS) of the breast has been a question of debate over the last decade. The aim of this study was to identify factors associated with microinvasive disease and determine the criteria for performing SLNB in patients with DCIS. Materials and Methods: 125 patients with DCIS who underwent surgery between January 2000 and December 2008 were reviewed to identify factors associated with DCIS and DCIS with microinvasion (DCISM). Results: 88 patients (70.4%) had pure DCIS and 37 (29.6%) had DCISM. Among 33 DCIS patients who underwent SLNB, one patient (3.3%) was found to have isolated tumor cells in her biopsy, whereas 1 of 14 (37.8%) patients with DCISM had micrometastasis (7.1%). Similarly, of 16 patients (18.2%) with pure DCIS and axillary lymph node dissection (ALND) without SLNB, none had lymph node metastasis. Furthermore, of 20 patients with DCISM and ALND, only one (5%) had metastasis. In multivariate analysis, the presence of comedo necrosis [relative risk (RR)=4.1, 95% confidence interval (CI)=1.6-10.6, P=0.004], and hormone receptor (ER or PR) negativity (RR=4.0, 95%CI=1.5-11, P=0.007), were found to be significantly associated with microinvasion. Conclusions: Our findings suggest patients presenting with a preoperative diagnosis of DCIS associated with comedo necrosis or hormone receptor negativity are more likely to have a microinvasive component in definitive pathology following surgery, and should be considered for SLNB procedure along with patients who will undergo mastectomy due to DCIS.

Secondary Tumors of the Urinary System: An Imaging Conundrum

Ali Devrim Karaosmanoglu,Mehmet Ruhi Onur,Musturay Karcaaltincaba,Deniz Akata,Mustafa Nasuh Ozmen 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.4

Imaging features of metastases to the urinary system may closely mimic primary urinary tract tumors, and differential diagnosis by imaging alone may be problematic or even impossible in some cases. The main purpose of this article was to amiliarize radiologists with imaging findings of metastasis to the urinary system on cross-sectional imaging, with an emphasis on abdominal and pelvic computed tomography and magnetic resonance imaging. In addition, we review the clinical importance and implications of metastases to the urinary tract and provide information on diagnostic work-ups.

Unusual Malignant Solid Neoplasms of the Kidney: Cross-Sectional Imaging Findings

Ali Devrim Karaosmanog˘lu,Mehmet Ruhi Onur,Ali Shirkhoda,Mustafa Ozmen,Peter F. Hahn 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.4

Malignant kidney neoplasms are the most frequently encountered solid kidney masses. Although renal cell carcinoma is the major renal malignancy, other solid malignant renal masses should be considered in the differential diagnosis of solid renal masses that do not contain a macroscopic fatty component. In this pictorial essay, we present the imaging findings of a primitive neuroectodermal tumor, primary liposarcoma of the kidney, primary neuroendocrine tumor, leiomyosarcoma, synovial sarcoma, malignant fibrous histiocytoma, sclerosing fibrosarcoma and renal metastasis of osteosarcoma.