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        Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis

        Ozan Ertekin,Meltem Kutnu,Aslı Aras Taşkın,Mustafa Demir,Ayten Yazgan Karataş,Gülay Özcengiz 한국미생물학회 2020 The journal of microbiology Vol.58 No.4

        Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted pMutin T3 insertion into the parental strain genome resulting in a genomic organization (bacA ::lacZ::erm::bacABCDEF) to form an IPTG-inducible bac operon. Although IPTG induction provided 3- to 5-fold increment in the transcription of bac operon genes, no bacilysin activity was detectable in bioassays and inability of the OGU1 to form bacilysin was confirmed by UPLC-mass spectrometry analysis. Phenotypic analyses revealed the deficiencies in OGU1 with respect to colony pigmentation, spore coat proteins, spore resistance and germination, which could be rescued by external addition of bacilysin concentrate into its cultures. 2DE MALDI-TOF/MS and nanoLC-MS/MS were used as complementary approaches to compare cytosolic proteomes of OGU1. 2-DE identified 159 differentially expressed proteins corresponding to 121 distinct ORFs. In nanoLCMS/ MS, 76 proteins were differentially expressed in OGU1. Quantitative transcript analyses of selected genes validated the proteomic findings. Overall, the results pointed to the impact of bacilysin on expression of certain proteins of sporulation and morphogenesis; the members of mother cell compartment- specific σE and σK regulons in particular, quorum sensing and two component-global regulatory systems, peptide transport, stress response as well as CodY- and ScoCregulated proteins.

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        Decolorization of antraquinonic dye, Reactive Blue 114 from synthetic wastewater by Fenton process: Kinetics and thermodynamics

        Mustafa Karatas,Yusuf Alparslan Argun,Mehmet Emin Argun 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.3

        In this work, the decolorization of C.I. Reactive Blue 114 (RB114), a commercially important anthraquinonic dye, by Fenton processes was investigated. The effects of operating parameters, such as Fe2+:H2O2 ratio, pH value, reaction time and temperature were examined. Maximum decolorization (86%) efficiencies were achieved at the Fe2+:H2O2 ratio of 0.1 and pH 3 for 20 min reaction time. The decolorization kinetic of RB114 followed pseudo-second-order reaction kinetic. The paper also discussed thermodynamic parameters including changes in Gibbs free energy, and activation energy for the decolorization of RB114 by Fenton and exposed that the oxidation process was spontaneous under natural conditions.

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        Adsorption of Reactive Blue 114 dye by using a new adsorbent: Pomelo peel

        Mehmet Emin Argun,Dunyamin Guclu,Mustafa Karatas 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3

        This paper describes the removal of Reactive Blue 114 dye from aqueous solutions by using pomelo(Citrus grandis) peel. Pomelo peel can be described as a new, low cost, abundantly available adsorbent. The optimum adsorbent mass, dye concentration, contact time and pH were determined in this study. The parameters of Langmuir, Freundlich and Temkin adsorption isotherms were also obtained usingconcentrations of the dyes ranging from 1.0 to 200 mg/L. Maximum adsorption capacity was obtained as16 mg/g at pH 2 and 303 K solution temperature. The adsorption process was observed to be reachingequilibrium after about 90 min.

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