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      저자 : Mushfiq, M. (검색결과 3 건)
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      • Antioxidant activity of water and alcohol extracts of Thuja orientalis leaves

        Nizam, Iram,Mushfiq, M Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.1

        Water and alcohol extracts were prepared from dried and powdered leaves of Thuja orientalis (T. orientalis). The reducing power, total phenolic content, the 1,1-diphenyl-2-picrylhydrazyl scavenging activity, inhibitory effect on Fe (II)-EDTA-$H_{2}O_{2}$ (Fenton reaction system) induced DNA damage and inhibitory effect on human red blood cell (RBC) hemolysis were evaluated in the present study. At a concentration of 200 mg, water and alcohol extracts of T. orientalis inhibited the hydrolysis of DNA by 72.859% and 65.312%, respectively. Water and alcohol extracts of T. orientalis also inhibited 2,2'-Azobis(2-amidinopropane) dihydrochloride induced RBC hemolysis to the extent of 69.30% and 54.55%, respectively. The reducing power and antioxidative activity of water extract was found to be more than that of alcohol extract. This is attributable to the presence of higher amount of phenolic compounds in water extract. The present results indicate that the T. orientalis extracts are rich sources of natural antioxidants and can protect DNA and human red blood cells against free radical induced oxidative damage.

      • KCI등재

        Antioxidant activity of five different solvent extracts of the edible fruits of Ficus racemosa

        Nizam, Iram,Mushfiq, M. 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.3

        The fruits of Ficus racemosa were extracted in five different solvents and their total phenolic content (TPC) was determined. They were then evaluated for their antioxidant activities by determining the reducing power, DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging, superoxide anion radical scavenging, inhibitory effect on Fenton reaction induced oxidative DNA damage and inhibition of ${\beta}$-carotene bleaching. The decreasing order of antioxidant activities is methanol extract (F-Me)>ethyl acetate extract (F-EA)>acetone extract (F-Ac)>chloroform extract (F-Chl)>petrol extract (F-Pet) in all the methods which is in conformity with TPC. The results clearly demonstrate that methanol extract has highest TPC and displayed strongest activity, and can be used to prevent oxidative stress related diseases. The processing of perishable fruits of Ficus racemosa by selective extraction with methanol can give better yield of antioxidants and the extract can be stored as food supplement with longer shelf life.

      • KCI등재후보

        Antioxidant activity of water and alcohol extracts of Thuja orientalis leaves

        Iram Nizam,M Mushfiq 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.1

        Water and alcohol extracts were prepared from dried and powdered leaves of Thuja orientalis (T. orientalis). The reducing power, total phenolic content, the 1,1-diphenyl-2-picrylhydrazyl scavenging activity, inhibitory effect on Fe (II)-EDTA-H2O2 (Fenton reaction system) induced DNA damage and inhibitory effect on human red blood cell (RBC) hemolysis were evaluated in the present study. At a concentration of 200 mg, water and alcohol extracts of T. orientalis inhibited the hydrolysis of DNA by 72.859% and 65.312%, respectively. Water and alcohol extracts of T. orientalis also inhibited 2,2’-Azobis(2-amidinopropane) dihydrochloride induced RBC hemolysis to the extent of 69.30% and 54.55%, respectively. The reducing power and antioxidative activity of water extract was found to be more than that of alcohol extract. This is attributable to the presence of higher amount of phenolic compounds in water extract. The present results indicate that the T. orientalis extracts are rich sources of natural antioxidants and can protect DNA and human red blood cells against free radical induced oxidative damage.

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