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        Nanotoxicity of Rare Earth Metal Oxide Anchored Graphene Nanohybrid: A Facile Synthesis and In-Vitro Cellular Response Studies

        Sriparna De,Smita Mohanty,Sanjay Kumar Nayak,Suresh K. Verma,Mrutyunjay Suar 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6

        Graphene, a single sp2 bonded carbon, is now a burgeoning interest with various fascinating properties in a large number of biomedical applications. Consequently, the impact of graphenebased functional nanohybrid and its potential risk to human health have raised considerable public concerns. In this present study, we have synthesized cerium oxide (CeO2) anchored reduced graphene oxide (RGO) nanohybrid and a detailed study on its nanotoxicity profile has also been scrutinized. To confirm the efficient synthesis of nano-CeO2/RGO nanohybrid, the systematic characterization has been carried out using FTIR, Raman and UV-Vis spectroscopic analysis. The successful imprint of CeO2 nanoparticles (NPs) on RGO nanosheet was also evident from the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) micrographs. A dose-dependent in vitro nanotoxicity of the nanohybrid has been assessed by using monocyte macrophage cells-Raw264.7 and colon cancer cells-HCT116 as compared with RGO and CeO2. The results conferred that as compared with single nanostructures (RGO or CeO2), nanohybrid showed excellent biocompatibility and no such prominence morphological alteration of the cell structure. Moreover, after exposure of different nanomaterials to HCT116 cells, the possible cellular interaction was investigated through reactive oxygen species (ROS) measurements using flow cytometry analysis dicholoro-dihydro-fluorescen dia-acetate (DCF) assay. These results conveyed that nanohybrid adapts an oxidative stress mechanism upon cellular interaction where it utilizes the scavenging property of CeO2, which induces the cell proliferation. Overall, the nano-CeO2/RGO nanohybrid exhibits a prolonged biocompatibility and cell viability, which is highly desired for biomedical applications.

      • KCI등재

        Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India

        Ananta N. Panda,Samir Ranjan Mishra,Lopamudra Ray,Surajit Das,Gurdeep Rastogi,Ajit Kumar Pattanaik,Tapan Kumar Adhya,Mrutyunjay Suar,Vishakha Raina 한국미생물학회 2018 The journal of microbiology Vol.56 No.4

        A moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1T was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0–25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C15:0 (44.89%), anteiso-C17:0 (6.18%), isoC15:0 (19.38%), and iso-C16:0 (7.39%). Several chemotaxonomic features conform the isolate be a member of genus Halobacillus. The isolate KGW1T contained A1γ meso-Dpm-direct type of peptidoglycan which is different from its phylogenetically closest neighbours. The 16S rRNA gene sequence based phylogenetic analysis also revealed the strain KGW1T was affiliated to the genus Halobacillus and sequence similarity between the isolated strain and the type strains of Halobacillus species were found closest to, H. dabanensis D-8 DSM 18199T (99.08%) and H. faecis IGA7-4 DSM 21559T (99.01%), H. trueperi SL-5 DSM 10404T (98.94%). The in silico DDH showed that the values in a range of 14.2–17.5% with the most closest strain H. dabanensis D-8 DSM 18199T and other type strains of the genus Halobacillus for which whole genome sequence is reported. DNA-DNA relatedness between strain KGW1T and the closest type strain Halobacillus trueperi DSM 10404T was 11.75% (± 1.15). The draft genome sequence includes 3,683,819 bases and comprises of 3898 predicted coding sequences with a G + C content of 46.98%. Thus, the significant distinctiveness supported by phenotypic and genotypic data with its closest neighbors and other closely related species confirm the strain KGW1T to be classified as a novel species within the genus Halobacillus, for which the name Halobacillus marinus sp. nov. is proposed. The type strain is KGW1T (= DSM 29522 = JCM 30443).

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        Taxonomic description and draft genome of Pseudomonas sediminis sp. nov., isolated from the rhizospheric sediment of Phragmites karka

        Pratiksha Behera,Madhusmita Mahapatra,Arman Seuylemezian,Parag Vaishampayan,V. Venkata Ramana,Neetha Joseph,Amaraja Joshi,Yogesh Shouche,Mrutyunjay Suar,Ajit K. Pattnaik,Gurdeep Rastogi 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

        The taxonomic position of a Gram-stain-negative, rod-shaped bacterial strain, designated PI11T , isolated from the rhizospheric sediment of Phragmites karka was characterized using a polyphasic approach. Strain PI11T could grow optimally at 1.0% NaCl concentration with pH 7.0 at 30°C and was positive for oxidase and catalase but negative for hydrolysis of starch, casein, and esculin ferric citrate. Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain PI11T belonged to the genus Pseudomonas sharing the highest sequence similarities with Pseudomonas indoloxydans JCM 14246T (99.72%), followed by, Pseudomonas oleovorans subsp. oleovorans DSM 1045T (99.29%), Pseudomonas toyotomiensis JCM 15604T (99.15%), Pseudomonas chengduensis DSM 26382T (99.08%), Pseudomonas oleovorans subsp. lubricantis DSM 21016T (99.08%), and Pseudomonas alcaliphila JCM 10630T (99.01%). Experimental DNA-DNA relatedness between strain PI11T and P. indoloxydans JCM 14246T was 49.4%. The draft genome of strain PI11T consisted of 4,884,839 bp. Average nucleotide identity between the genome of strain PI11T and other closely related type strains ranged between 77.25–90.74%. The polar lipid pattern comprised of phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine. The major (> 10%) cellular fatty acids were C18:1 ω6c/ω7c, C16:1 ω6c/ω7c, and C16:0. The DNA G + C content of strain PI11T was 62.4 mol%. Based on the results of polyphasic analysis, strain PI11T was delineated from other closely related type strains. It is proposed that strain PI11T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas sediminis sp. nov. is proposed. The type strain is PI11T (= KCTC 42576T = DSMZ 100245T).

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