Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

The clonal origins of leukemic progression of myelodysplasia

Kim, T,Tyndel, M S,Kim, H J,Ahn, J-S,Choi, S H,Park, H J,Kim, Y-k,Yang, D-H,Lee, J-J,Jung, S-H,Kim, S Y,Min, Y H,Cheong, J-W,Sohn, S K,Moon, J H,Choi, M,Lee, M,Zhang, Z,Kim, D(D H) Nature Publishing Group 2017 Leukemia Vol.31 No.9

<P>The genetics behind the progression of myelodysplasia to secondary acute myeloid leukemia (sAML) is poorly understood. In this study, we profiled somatic mutations and their dynamics using next generation sequencing on serial samples from a total of 124 patients, consisting of a 31 patient discovery cohort and 93 patients from two validation cohorts. Whole-exome analysis on the discovery cohort revealed that 29 of 31 patients carry mutations related to at least one of eight commonly mutated pathways in AML. Mutations in genes related to DNA methylation and splicing machinery were found in T-cell samples, which expand at the initial diagnosis of the myelodysplasia, suggesting their importance as early disease events. On the other hand, somatic variants associated with signaling pathways arise or their allelic burdens expand significantly during progression. Our results indicate a strong association between mutations in activated signaling pathways and sAML progression. Overall, we demonstrate that distinct categories of genetic lesions play roles at different stages of sAML in a generally fixed order.</P>

Investigation of the Structural, Electrical, and Optical Properties of MnAl2Se4 Layers Grown Using the Hot-Wall Deposition Technique

S. H. You,K. J. Hong,J. W. Jeong,T. S. Jeong,C. J. Youn,J. D. Moon 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.4

MnAl2Se4 layers were grown using the hot-wall deposition technique with an attached reservoir tail. Precise control of the vapor pressure in the reservoir was thought to play an important role in the grown of a stoichiometric layer. From the relation between the reciprocal temperature and the carrier concentration, we extracted the dominant trap level as 96.1 meV in the high-temperature region and 13.9 meV in the middle-temperature region. Thus, from a log-log plot between the mobility and the temperature, the mobility showed the different temperature-dependent decreases of the mobility at temperatures above 100 K: T−1/2 in the temperature range of 100 < T < 225 K and T−3/2 in the temperature of T > 225 K. The mobility decreased in proportion to T1 in the low-temperature range of T < 100 K. By analyzing the optical absorption results, the bandgap variation matched Eg(T) = Eg(0) − 3.19 × 10−3 T2/(T + 488) well, where Eg(0) is estimated to be 3.5616 eV. Consequently, low-temperature growth of MnAl2Se4 layers was achieved by using the hot-wall deposition technique.

육계분 발효사료의 첨가수준이 숫꽃사슴의 소화율, 건물채식량 및 질소출납에 미치는 영향

전병태,곽완섭,강성기,이상무,문상호 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 실험은 기존 사슴사료에 있어서 육계분 발효사료의 대체 가능성을 검토하기 위해 숫사슴에 있어서 육계분 발효사료의 첨가수준을 0, 15 및 30%로 달리하여 소화율, 증체량, 건물채식량 및 질소출납에 미치는 영향을 검토하였다. 모든 처리구에서 실험사슴들은 비슷한 수준의 채식량을 나타내어 건물기준으로 체중의 약 3% 정도의 채식량을 기록하고 있어 육계분 발효사료를 30%까지 첨가한 경우 숫사슴의 채식 기호도에는 부정적인 영향이 나타나지 않는 것으로 판단되었다. 건물소화율은 Control이 76.5%로 가장 높았고, T2가 70.3%로 가장 낮아 유의성(P<0.05)이 인정되었다. 조단백질의 경우도 Control구가 75.8%로 가장 높은 소화율을 나타내었으며, T2가 70.2%로 가장 낮은 소화율을 나타내어 처리간에 유의성(P<0.05)이 인정되었다. 조섬유 소화율은 Contol구 70.8%, T1구 62.7%, T2구 56.0%로 육계분 발효사료의 첨가수준이 높아짐에 따라 조섬유 소화율이 저하되어 Control구와의 사이에 유의성(P<0.05)이 인정되었다. 건물 및 가소화 건물섭취량 모두 육계분 발효사료의 첨가수준이 높아질수록 약간씩 저하되는 경향은 있었으나 그 차이에 대한 유의성은 인정되지 않았다. 실험사슴들의 일당 증체량은 T1구에서 가장 높았으며 Control구에서 가장 낮았으나 처리간의 차이는 유의성이 인정되지 않았다. 질소섭취량은 T1구가 40.2g으로 가장 많았으며 T2구가 38.0g으로 가장 낮았으나 처리간의 유의성은 인정되지 않았다. 분 중 질소의 양은 T2구에서 가장 높았으며 뇨 중 질소의 양은 Control구가 가장 높았다(P<0.05). 체내 질소 축적량은 T1구에서 가장 높았으나 나머지 처리구들과 비슷한 수준을 유지했다. To determine nutritive value and suitability of deep-stacked broiler litter for deer, influence of supplementary levels of broiler litter on digestibility, dry matter intake, and nitrogen balance was investigated in male spotted deer (Cervus nippon) fed a commercial mixed(complete) ration. The deep-stacked and ground broiler litter was supplemented at the level of 0%(Control). 15%(TI) and 30%(T2) on the basis of fresh matter to the mixed ration. All treatments had similar palatability at about 3% body weigh of dry matter intake. Dry matter digestibility was highest in Control and lowest in T2(P<0.05). Digestibility of crude protein was lowest(P<0.05) in T2. Dry matter and digestible dry matter intake was similar in all treatments. Daily body weight gain was not affected by treatments. Feeding 15 or 30% of broiler litter to male deer resulted in similar nitrogen intake. fecal nitrogen excretion and daily nitrogen retention(g/d), compared with feeding Control. These results suggest that up to 30% of broiler litter may be fed to male deer without deleterious effects on deer performance.

Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium

Lee, E.H.,Moon, K.E.,Kim, T.G.,Lee, S.D.,Cho, K.S. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.120 No.6

Kinetic and enzymatic inhibition experiments were performed to investigate the effects of methanethiol (MT) and hydrogen sulfide (H<SUB>2</SUB>S) on methane oxidation by a methane-oxidizing consortium. In the coexistence of MT and H<SUB>2</SUB>S, the oxidation of methane was delayed until MT and H<SUB>2</SUB>S were completely degraded. MT and H<SUB>2</SUB>S could be degraded, both with and without methane. The kinetic analysis revealed that the methane-oxidizing consortium showed a maximum methane oxidation rate (V<SUB>max</SUB>) of 3.7 mmol g-dry cell weight (DCW)<SUP>-1</SUP> h<SUP>-1</SUP> and a saturation constant (K<SUB>m</SUB>) of 184.1 μM. MT and H<SUB>2</SUB>S show competitive inhibition on methane oxidation, with inhibition values (K<SUB>i</SUB>) of 1504.8 and 359.8 μM, respectively. MT was primary removed by particulate methane monooxygenases (pMMO) of the consortium, while H<SUB>2</SUB>S was degraded by the other microorganisms or enzymes in the consortium. DNA and mRNA transcript levels of the pmoA gene expressions were decreased to ~10<SUP>6</SUP> and 10<SUP>3</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after MT and H<SUB>2</SUB>S degradation, respectively; however, both the amount of the DNA and mRNA transcript recovered their initial levels of ~10<SUP>7</SUP> and 10<SUP>5</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after methane oxidation, respectively. The gene expression results indicate that the pmoA gene could be rapidly reproducible after methane oxidation. This study provides comprehensive information of kinetic interactions between methane and sulfur compounds.

Association of tumor necrosis factor- gene polymorphisms with advanced stage endometriosis

Lee, G. H.,Choi, Y. M.,Kim, S. H.,Hong, M. A.,Oh, S. T.,Lim, Y. T.,Moon, S. Y. Oxford University Press 2008 Human reproduction Vol.23 No.4

<P>BACKGROUND: This study was performed to investigate whether specific haplotypes and several single nucleotide polymorphisms in the promoter region of the tumor necrosis factor (TNF)-alpha gene are associated with the risk of advanced stage endometriosis in a Korean population. METHODS: This study comprised women with (n = 246) or without (n = 248) endometriosis. The TNF:g.[-1031T > C], TNF:g.[-863C > A] and TNF:g.[-857C > T] polymorphism in the TNF-alpha gene were assessed by PCR-restriction fragment length polymorphism analysis, which utilized digestion by BbsI, HypCH4IV and HypCH4IV restriction enzymes, respectively. In silico haplotypes were deduced by using the Haploview version 3.32. RESULTS: The genotype distribution of TNF:g.[-1031T > C] was significantly different between total endometriosis patients and the controls (T/T of 56.9 versus 60.1%, T/C of 35.4 versus 37.5% and C/C of 7.7 versus 2.4%, respectively, P = 0.027). This difference at the TNF:g.[-1031T > C] tends to increase in Stage IV endometriosis (P = 0.01). However, there was no difference in the TNF:g.[-863C > A] and TNF:g.[-857C > T] site between the two groups. Even when the endometriosis cases were subdivided into American Society for Reproductive Medicine Stages III and IV, genotype differences were not found. The CC homozygote at TNF:g.-863 was more frequently found in the controls than Non-CC group (P = 0.04; odds ratio = 0.67; 95% confidence interval = 0.45-0.98). All haplotypes and diplotypes, deduced by in silico analysis, showed no association with subgroups or controls. CONCLUSIONS: Our results suggest that the genotype frequencies at the TNF:g.[-1031T > C] and the TNF:g.[-863C > A] sites may be associated with advanced stage endometriosis in the Korean population.</P>

톱밥 발효사료 급여 및 분말어유의 첨가사료가 돈육의 이화학적 성질에 미치는 영향

이정일,문점동,박구부,박범영,박태선,황보종,이한기,진상근,김영직 한국축산학회 1997 한국축산학회지 Vol.39 No.5

This study was conducted to investigate the influence of pork quality pigs which were fed with diets supplemented with fermented sawdust and sardin powder oil. The pigs were randomly assigned to one of the four treatments: a), control (commercial feed); b), T1 (30% fermented sawdust); c), T2 (10% sardin powder oil) d), T3 (30% fermented sawdust, l0% sardin powder oil and 30% limited amino acid) from 30 kg to 110 kg B. Wt. and slaughtered. The samples were stored at 0±1 C. The physico-chemical properties were determined at the dust of 2, 4, 6, 8 and 15. For the loin the pH's of all the treatments on the 15th day were higher than those of the 2nd day, and that of TI was higher than those of the others. The total moisture contents of all treatments decreased with the storage (P$lt;0.05), and there were no significant differences among treatments on the 15th day. The water soluble proteins of all treatments increased with time and were highest on the 8th day and decreased gianibicanth thereater. T1 had significantly higher water soluble protein than others(P$lt;0.05). The salt soluble proteins of all treatments increased with lure the days(P$lt;0.05). T1 had higher salt soluble protein than others during the storage period(P$lt;0.05). The brittleness, hardness, cohesiveness, gumminess and chewiness of all the treatments were not significantly different among during the period, but elasticity of them increased after from the 2nd day. The brittleness of control was higher than that of the others on the 15th day, and the hardness and chewiness of control were higher than those of the others on the 6th day(P$lt;0.05). Forthebelly the pH's of all the treatments on the 15th day were higher than those of the at 2nd day(P$lt;0.05). T1 showed higher pH than the others during the period(P$lt;0.05). The total moisture contents of control and T1 were significantly decreased with time and there were no significant differences between T2 and T3. T1 showed the highest value. The water soluble proteins of all the treatments decreased with time and was lower on the 15th day(P$lt;0.05). That of control and T1 was significantly higher than that of others on the 2nd day, but there were no significant differences among the treatments on the 15th day. The salt soluble proteins of all treatments increased with time (P$lt;0.05), and they were highest on the 15th day. T1 had significantly higher salt soluble protein than the others on the 8th and 15th days.

Sorghum × Sudangrass hybrid 의 입모저장에 관한 연구 ; 1 . 입모저장기간이 생육특성 및 기호성 변화에 미치는 영향

이상무 ( S M Lee ),문상호 ( S H Moon ),백인철 ( I C Baek ),전병태 ( B T Jeon ) 한국축산학회 1994 한국축산학회지 Vol.36 No.1

Growth characteristics and palatability by different harvesting dates were measured in order to determine its availability and effective storage period when sorghum × sudangrass hybrid were stored as foggage. Experiment was carried out with six treatments as follows; T1, T2 and T3 were foggaged at 120, 160 and 200㎝ in plant length and T4, T5 and T6 were foggaged at boot, milk and dough stage on 7 october after regrowth respectively. Rice straw was offered to examine the relative palatability a, control. 1. Growth characteristics such as plant length, leaf length, leaf width, leaf number and stem hardness were decreased with prolongation of foggage period at all treatments. Especially, decrease and falling ratio were a heigher at T5 and T6 than the other treatments. 2. Greeness grade of leaf showed fast decoloration at TI and T2 treatments, but stem showed fast decoloration at TS and T6 treatments. Leaf and stem decolorized in mid-October and mid-november in all treatments. 3. Leaf ratio was deceased with prolongation of foggage period, but stem showed gradually increase. Grain ratio was stable increase at T6 treatments, but not at T5 treatment, 4. Dry matter contents of leaf and grain were stable increased at all treatment but stem was multifarious according to weather conditions. 5. Changes of palatability were high at T1, T2 and T3 treatments untill mid-October, but T4 treatment and rice straw showed high in early December. 6. According to the results obtained in this study, it was suggested that when sorghum x sudangrass hybrid is used as foggage, foggaging period would be suitable until mid-november and growth stage for foggaging would be recommendable from T2 to boot stage for maximum availability.

Thymol from Thymus quinquecostatus Celak. protects against tert-butyl hydroperoxide-induced oxidative stress in Chang cells

Kim, Y. S.,Hwang, J. W.,Kang, S. H.,Kim, E. H.,Jeon, Y. J.,Jeong, J. H.,Kim, H. R.,Moon, S. H.,Jeon, B. T.,Park, P. J. Springer Science + Business Media 2014 Journal of Natural Medicines Vol.68 No.1

The present work describes the protective effects of thymol isolated from Thymus quinquecostatus Celak. against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage through various experiments with Chang liver cells. Thymol significantly protected hepatocytes against t-BHP-induced cell cytotoxicity as demonstrated by increased viability. Furthermore, observation of Hoechst staining, annexin V/PI staining, and expression of Bcl-2 and Bax indicated that thymol inhibited t-BHP-induced Chang cell damage. Further, thymol inhibited the loss of mitochondrial membrane potential in t-BHP-treated Chang cells and prevented oxidative stress-triggered reactive oxygen species (ROS) and lipid peroxidation (malondialdehyde, MDA). Thymol restored the antioxidant capability of hepatocytes including glutathione (GSH) levels which were reduced by t-BHP. These results indicated that thymol prevents oxidative stress-induced damage to liver cells through suppression of ROS and MDA levels and increase of GSH level.

Lower-But-Normal Serum TSH level Is Associated With the Development or Progression of Cognitive Impairment in Elderly: Korean Longitudinal Study on Health and Aging (KLoSHA)

Moon, J.H.,Park, Y.J.,Kim, T.H.,Han, J.W.,Choi, S.H.,Lim, S.,Park, D.J.,Kim, K.W.,Jang, H.C. ENDOCRINE SOCIETY 2014 The Journal of clinical endocrinology & metabolism Vol.99 No.2

Context: The association between subclinical hyperthyroidism and the risk of dementia has been validated in several studies. However, the effect of thyroid function within reference range on the risk of cognitive dysfunction including mild cognitive impairment (MCI) and dementia is still unclear.Objective: Our aim was to investigate the association between thyroid function and the risk of MCI and dementia in euthyroid elderly subjects.Design, Setting, and Participants: We conducted a population-based prospective study as a part of the Korean Longitudinal Study on Health and Aging. A total of 313 participants who were euthyroid and nondemented at baseline and completed cognitive function tests at a 5-year follow-up evaluation (mean age 72.5 +/- 6.9 y) were analyzed in the present study.Main Outcome Measure: Baseline thyroid function was compared according to the development of MCI or dementia during the study period. Binary logistic regression analysis was performed to investigate the independent association between thyroid function and cognitive impairment.Results: At baseline evaluation, 237 subjects were cognitively normal, and 76 subjects had MCI. Diagnoses of cognitive function in 259 subjects remained unchanged or improved during the study period (nonprogression group), whereas 54 subjects showed progression of cognitive impairment to MCI or dementia (progression group). In the progression group, baseline serum TSH levels were lower than those in nonprogression group. Baseline serum free T-4 levels were not significantly different between these two groups. The association between lower baseline serum TSH levels and the development of MCI or dementia was maintained after adjustment for conventional baseline risk factors.Conclusions: Lower serum TSH level within the reference range was independently associated with the risk of cognitive impairment including MCI and dementia in elderly subjects.