Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

일본적 생산시스템의 구조와 국제적 논쟁에 관한 일 고찰

주무현 慶北大學校出版部 1998 經商論集 Vol.26 No.2

국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석

박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

무술 스포츠로서의 택견의 정신에 대한 고찰

최무현,김동준 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

Accroding to results about the mental a of Takkyun as a martial sport, We can make following conclusions. 1. The modern martial arts are the concept which is added to sports, When we indicated one of them, we use the word, "martial sports". 2. The modern Korean martial ideologies were under the influence of Japanese martail as they are, Japeneses martail ideologies are hierarchism but Korean martial characteristic are democritism. 3. Takkyun keeps Korean essential martial values like them. 4. Takkyun has been industrialized all over the world from long time ago. That is, Takkyun is one of the martial sports. 5. The martial mental of Takkyun has only mutual coexisting and democratic values which are based on the liberty and equality.

신체활동에 따른 요통 예방과 치료에 관한 자세의 생활지도 및 운동요법 연구 고찰

최무현,정대철 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The spine is a pillar of human body. The lumber connects upper body and lower body, protects spinal marrow, and sustain body weight. The relative posture of the discs changes according to the spine's movement and body's postural transformation, The pain, pressure, and temperature are felt through the spinal nerves, When a certain posture or a movement is maintained for a long time, the spinal nerves are extended too much and pain occurs, So, the normal right arrangement of the discs cannot be maintained, A certain movement is sometimes restricted partly or wholly. when the movement is tried, severe pain is felt, To recover normal flexibility of the spines, we should do proper exercise. The general reason of low back pain is the failure of maintaining proper spinal curve. When you stand, sit clown, or lie in a tensed posture or in a bad posture too long, you are prone to have low back pain When your working position requires you to bend your spine for a long time, the same problem occurs, The furniture of which design ignores human body engineering consideration also causes low back pain. Everyday life posture guidance includes the posture guidance of human dynamics, the posture instruction on the bed, using medical gymnastics, and the postural instruction at the recreational events as a sport activity. To prevent low back pain, the normal right spinal curve should be maintained according to each posture and the relative muscles of the lumber should be trained, Spinal muscles, abdominal muscles, and back muscles should be strengthened all together to decentralize body weight that is usually loaded onto the spine, The burden on the spine should be distributed to the waist or abdominal muscles, The muscle training is a very important and effective method to prevent low back pain.

아쿠아 런닝의 치료적 운동처방 원리 적용에 관한 고찰

최무현 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

These researches make the following results about principle's applications of aqua running for athletic rehabilitation. Firstly, water treatment in principle's application can be composed of ideal environment by Buoyancy, Drag force, Hydrostatic pressure, Specific heat and Temperature. Secondly, in Biomechanics of aqua running each general physiological reaction shows up working in the height of water level and the attitude of running inside of water. Thirdly, we can get higher intussusception rations of oxygen than running on the land through aqua running keeping proper, planimetries of maintenance of walking, combined exercises of interval of leg and arms attract rehabilitation for running on the land. fourthly, There are interactive relations between heart rate and oxygen-uptake, Rating of per-ceived exertion is made up of aqua running's five steps of Brennan suale.

여성들의 크레아틴 섭취에 따른 근력 및 신체구성에 미치는 영향

최무현,도길명 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The purpose of this study was to determine the effectiveness of creatine ingestion during weight training on the dynamic muscular strength and change of body composition in women athletes. Ten collegiate women majoring physical education consumed either creatine (n=6) or a placevo(n=6) for 5 weeks during off season training (20g/day for 1week and 4weeks). Strength tests performed were the 1-RM bench press and leg extension. An analysis on liver enzymes consisted of GGL and BUN. Body composition measurements were performed by skinfold caliper and body components analyzer. Conclusions obtained from this study were as follows; Oral creatine ingestion in crease muscular strength for the upper body, while did not influence body weight or lean body mass gain in women doing weight training for 5 weeks.

노동운동과 정치운동의 맥락적 의존성 : 부마민주항쟁 사례연구

주무현 경상대학교 사회과학연구소 2003 사회과학연구 Vol.21 No.-

I analyse the contextual dependency between labour movement and political movement in the case of the Busan and Masan Areas' democratic movement of 1979. This paper focuses on the concept of political and industrial opportunity structure as a determinant of labour movement an political movement. Busan and Masan Areas' democratic movement depended contextually on the growth and development of the democratic union movement of 1970s. The success of the political movement opened the political opportunity structure of labour movement of 1980 in Korea. At same time Korean labour movement was contextually determined by the political movement as such Busan and Masan Areas' democratic Movement. In conclusion, the success of labour movement will depend on how it use the political opportunity structure in Korea.