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- 저자 : Montini, Tiziano (검색결과 2 건)
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Bellucci Linda,Montini Giovanni,Collino Federica,Bussolati Benedetta 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.4
Background: Dynamic cultures, characterized by continuous fluid reperfusion, elicit physiological responses from cultured cells. Mesenchymal stem cell-derived EVs (MSC-EVs) has been proposed as a novel approach in treating several renal diseases, including acute glomerular damage, by using traditional two-dimensional cell cultures and in vivo models. We here aimed to use a fluidic three-dimensional (3D) glomerular model to study the EV dynamics within the glomerular structure under perfusion. Methods: To this end, we set up a 3D glomerular model culturing human glomerular endothelial cells and podocytes inside a bioreactor on the opposite sides of a porous membrane coated with type IV collagen. The bioreactor was connected to a circuit that allowed fluid passage at the rate of 80 µl/min. To mimic glomerular damage, the system was subjected to doxorubicin administration in the presence of therapeutic MSC-EVs. Results: The integrity of the glomerular basal membrane in the 3D glomerulus was assessed by a permeability assay, demonstrating that the co-culture could limit the passage of albumin through the filtration barrier. In dynamic conditions, serum EVs engineered with cel-miR-39 passed through the glomerular barrier and transferred the exogenous microRNA to podocyte cell lines. Doxorubicin treatment increased podocyte apoptosis, whereas MSC-EV within the endothelial circuit protected podocytes from damage, decreasing cell death and albumin permeability. Conclusion: Using an innovative millifluidic model, able to mimic the human glomerular barrier, we were able to trace the EV passage and therapeutic effect in dynamic conditions. Background: Dynamic cultures, characterized by continuous fluid reperfusion, elicit physiological responses from cultured cells. Mesenchymal stem cell-derived EVs (MSC-EVs) has been proposed as a novel approach in treating several renal diseases, including acute glomerular damage, by using traditional two-dimensional cell cultures and in vivo models. We here aimed to use a fluidic three-dimensional (3D) glomerular model to study the EV dynamics within the glomerular structure under perfusion. Methods: To this end, we set up a 3D glomerular model culturing human glomerular endothelial cells and podocytes inside a bioreactor on the opposite sides of a porous membrane coated with type IV collagen. The bioreactor was connected to a circuit that allowed fluid passage at the rate of 80 µl/min. To mimic glomerular damage, the system was subjected to doxorubicin administration in the presence of therapeutic MSC-EVs. Results: The integrity of the glomerular basal membrane in the 3D glomerulus was assessed by a permeability assay, demonstrating that the co-culture could limit the passage of albumin through the filtration barrier. In dynamic conditions, serum EVs engineered with cel-miR-39 passed through the glomerular barrier and transferred the exogenous microRNA to podocyte cell lines. Doxorubicin treatment increased podocyte apoptosis, whereas MSC-EV within the endothelial circuit protected podocytes from damage, decreasing cell death and albumin permeability. Conclusion: Using an innovative millifluidic model, able to mimic the human glomerular barrier, we were able to trace the EV passage and therapeutic effect in dynamic conditions.
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Photocatalytic decolourization of dyes on NiO-ZnO nano-composites
Hameed, Abdul,Montini, Tiziano,Gombac, Valentina,Fornasiero, Paolo Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.5
NiO-ZnO nanocomposite photocatalysts were synthesized by co-precipitation/co-gel formation techniques using potassium hydroxide (KOH), sodium carbonate ($Na_2CO_3$) and oxalic acid ($H_2C_2O_4$) as precipitating agents. X-Ray diffraction (XRD) analysis was employed for the determination of crystallite size and phase purity, while BET surface areas were estimated by nitrogen physisorption analysis. The photocatalytic activities of the NiO-ZnO nanocomposites, evaluated using the photo-decolourization of two structurally different dyes, methyl orange and methylene blue, were correlated with the variation in the crystallite size of the constituents of the composites. All the three synthesized nanocomposites showed appreciable activity for the decolourization of dyes. NiO-ZnO nanocomposite derived by KOH exhibited maximum activity for the decolourization of both the dyes and the decolourization rate of methylene blue was found to be comparable with that of Degussa P-25 $TiO_2$. Finally, the activity of the most active NiO-ZnO composite with relatively large crystallite sizes was compared with the pure components and their physical mixture, synthesized by the identical synthetic route, in the same molar ratio as that of nanocomposite.
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