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IMPROVED APPROACH FOR THE LABELING OF ARGININE^ GLUTAMIC^ AND ASPARTIC ACID SIDE CHAINS IN PROTEINS USING CHROMATOGRAPHIC TECHNIQUES
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복잡한 3차원 비행체 주위의 격자계 형성
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Baer-Kaplansky Theorem for Modules over Non-commutative Algebras
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한국경제연구원 Issue Paper
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Population differences in susceptibility to AIDS: An evolutionary analysis
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龍潭댐과 全州圈 用水需要 및 供給
- 검색키워드
- 저자 : Mohsen Tabasi (검색결과 2 건)
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Mohsen Tabasi,Mohammad Reza Asadi Karam,Mehri Habibi,Mir Saeed Yekaninejad,Saeid Bouzari 질병관리본부 2015 Osong Public Health and Research Persptectives Vol.6 No.4
Objectives: Urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern. Methods: UPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded. Results: Of the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients’ profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers. Conclusion: This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients’ profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.
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Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus
Ahmad Nasser,Reza Azizian,Mohsen Tabasi,Jamil Kheirvari Khezerloo,Fatemah Sadeghpour Heravi,Morovat Taheri Kalani,Norkhoda Sadeghifard,Razieh Amini,Iraj Pakzad,Amin Radmanesh,Farid Azizi Jalilian 질병관리본부 2019 Osong Public Health and Research Persptectives Vol.10 No.1
Objectives: The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Methods: Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Results: Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order. Conclusion: Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.
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